################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BalI BamHI BbeI BbsI BbvCI BciVI BclI BfaI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MamI MboI MfeI MflI MluI MluNI MscI MspA1I MunI MvnI NarI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 392 TCCGGA T^CCGGA AcsI 442 AAATTC [GA]^AATT[CT] AflII 295 CTTAAG C^TTAAG ApoI 442 AAATTC [GA]^AATT[CT] AspI 138 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 205 CCCGGG C^[CT]CG[GA]G BanI 91 GGTGCC G^G[CT][GA]CC BanII 26 GAGCCC G[GA]GC[CT]^C BbrPI 512 CACGTG CAC^GTG BfrI 295 CTTAAG C^TTAAG BsaAI 512 CACGTG [CT]AC^GT[GA] BsaWI 392 TCCGGA [AT]^CCGG[AT] BseAI 392 TCCGGA T^CCGGA BsmAI 272 GAGAC GTCTC(1/5) BsoBI 205 CCCGGG C^[CT]CG[GA]G BspDI 541 ATCGAT AT^CGAT BspEI 392 TCCGGA T^CCGGA BsrFI 57 GCCGGC [GA]^CCGG[CT] BssSI 310 CACGAG CACGAG(-5/-1) CfoI 255 GCGC GCG^C Cfr10I 57 GCCGGC [GA]^CCGG[CT] ClaI 541 ATCGAT AT^CGAT EaeI 357 CGGCCA [CT]^GGCC[GA] EarI 501 GAAGAG CTCTTC(1/4) HhaI 255 GCGC GCG^C HinP1I 255 GCGC G^CGC Ksp632I 501 GAAGAG CTCTTC(1/4) MaeII 513 ACGT A^CGT MroI 392 TCCGGA T^CCGGA MseI 296 TTAA T^TAA NaeI 57 GCCGGC GCC^GGC NgoMIV 57 GCCGGC G^CCGGC NspI 243 GCATGT [GA]CATG^[CT] PflFI 138 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 512 CACGTG CAC^GTG PmlI 512 CACGTG CAC^GTG SfcI 481 CTACAG C^T[GA][CT]AG SmaI 205 CCCGGG CCC^GGG SmlI 295 CTTAAG C^T[CT][GA]AG TaqI 542 TCGA T^CGA Tru9I 296 TTAA T^TAA Tsp509I 443 AATT ^AATT Tth111I 138 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 542 TCGA T^CGA XmaCI 205 CCCGGG C^CCGGG XmaI 205 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AflIII 511 ACACGT A^C[GA][CT]GT 513 ACGTGT AvaII 47 GGACC G^G[AT]CC 83 GGTCC BcgI 271 CGAGACTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 533 GCATATCCATCG BmyI 26 GAGCCC G[AGT]GC[ACT]^C 192 GGGCAC BpmI 162 CTCCAG CTGGAG(16/14) 220 CTGGAG BseRI 37 CTCCTC GAGGAG(10/8) 211 GAGGAG BsmFI 46 GGGAC GGGAC(10/14) 84 GTCCC Bsp1286I 26 GAGCCC G[AGT]GC[ACT]^C 192 GGGCAC BsrI 275 ACTGG ACTGG(1/-1) 360 CCAGT BstXI 150 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 539 CCATCGATTTGG Cac8I 57 GCCGGC GC[ACGT]^[ACGT]GC 431 GCAAGC DdeI 129 CTCAG C^T[ACGT]AG 140 CTCAG EcoT14I 87 CCAAGG C^C[AT][AT]GG 183 CCAAGG MboII 498 GAAGA GAAGA(8/7) 501 GAAGA PpuMI 46 GGGACCC [GA]G^G[AT]CC[CT] 82 GGGTCCC StyI 87 CCAAGG C^C[AT][AT]GG 183 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI BbvI BcnI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeIII MnlI MslI MspI MvaI MwoI NciI NlaIII NlaIV PleI Sau96I ScrFI TseI Tsp45I TspRI