Restriction map for
pFN21AE3045
(Circular) (Six-base) MAPSORT of: pFN21AE3045.seq Check: 9604 from: 1 to: 5257
pFN21AE3045 5257 bp
With 182 enzymes: SgfI *
March 28, 2012 12:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3067 278
Size: 53 83 186 936 1531 2468
Fragments arranged by size:
2468 1531 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2600 1937
Size: 663 4594
Acc65I G'GTAC_C
Cuts at: 2578 2578
Size: 5257
AclI AA'CG_TT
Cuts at: 1563 3384 3757 1563
Size: 1821 373 3063
Fragments arranged by size:
3063 1821 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2091 2291 3320 4704 4972 1632
Size: 459 200 1029 1384 268 1917
Fragments arranged by size:
1917 1384 1029 459 268 200
AflII C'TTAA_G
Cuts at: 820 1017 2300 820
Size: 197 1283 3777
Fragments arranged by size:
3777 1283 197
AflIII A'CryG_T
Cuts at: 1203 2516 2518 4156 5133 1203
Size: 1313 2 1638 977 1327
Fragments arranged by size:
1638 1327 1313 977 2
AgeI A'CCGG_T
Cuts at: 4088 4088
Size: 5257
AhdI GACnn_n'nnGTC
Cuts at: 3986 3986
Size: 5257
AleI CACnn'nnGTG
Cuts at: 1166 2519 1166
Size: 1353 3904
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2222 1184
Size: 1038 4219
AlwNI CAG_nnn'CTG
Cuts at: 3000 4572 3000
Size: 1572 3685
ApaLI G'TGCA_C
Cuts at: 3314 4470 3314
Size: 1156 4101
ApoI r'AATT_y
Cuts at: 1493 2447 2566 2792 2944 5053 1493
Size: 954 119 226 152 2109 1697
Fragments arranged by size:
2109 1697 954 226 152 119
AseI AT'TA_AT
Cuts at: 160 1032 3811 160
Size: 872 2779 1606
Fragments arranged by size:
2779 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5257
AvaI C'yCGr_G
Cuts at: 1870 1943 2210 2582 1870
Size: 73 267 372 4545
Fragments arranged by size:
4545 372 267 73
BamHI G'GATC_C
Cuts at: 2587 2954 2587
Size: 367 4890
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2578 4033 618
Size: 325 217 627 309 482 1455 1842
Fragments arranged by size:
1842 1455 627 482 325 309 217
BanII G_rGCy'C
Cuts at: 1391 2035 2576 1391
Size: 644 541 4072
Fragments arranged by size:
4072 644 541
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2265 2299 2527 2561 3448 3482 1903
Size: 34 328 34 228 34 887 34 3678
Fragments arranged by size:
3678 887 328 228 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3149 4359 3149
Size: 1210 4047
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5257
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3868 136
Size: 107 122 71 631 605 2196 1525
Fragments arranged by size:
2196 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5252 5252
Size: 5257
BlpI GC'TnA_GC
Cuts at: 2674 2674
Size: 5257
Bme1580I G_kGCm'C
Cuts at: 1790 2201 3318 4474 1790
Size: 411 1117 1156 2573
Fragments arranged by size:
2573 1156 1117 411
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5257
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2988 3946 442
Size: 416 53 83 399 24 1571 958 1753
Fragments arranged by size:
1753 1571 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2152 2246 3917 1239
Size: 913 94 1671 2579
Fragments arranged by size:
2579 1671 913 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3382 4247 4545 3382
Size: 865 298 4094
Fragments arranged by size:
4094 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3920 882
Size: 617 2421 2219
Fragments arranged by size:
2421 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2519 3048 493
Size: 711 1315 529 2702
Fragments arranged by size:
2702 1315 711 529
BsaBI GATnn'nnATC
Cuts at: 5251 5251
Size: 5257
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3064 3446 4081 275
Size: 53 83 186 936 1531 382 635 1451
Fragments arranged by size:
1531 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2397 3690 4088 4362 4509 4920 2397
Size: 1293 398 274 147 411 2734
Fragments arranged by size:
2734 1293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5032
Fragments arranged by size:
5032 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2231 1186
Size: 847 198 4212
Fragments arranged by size:
4212 847 198
BseYI C'CCAG_C
Cuts at: 2121 2154 2292 4460 4956 2121
Size: 33 138 2168 496 2422
Fragments arranged by size:
2422 2168 496 138 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4684
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2707 3468 4496 1540
Size: 275 186 706 761 1028 2301
Fragments arranged by size:
2301 1028 761 706 275 186
BsiHKAI G_wGCw'C
Cuts at: 3318 3403 4474 3318
Size: 85 1071 4101
Fragments arranged by size:
4101 1071 85
BsmI GAATG_Cn'
Cuts at: 2768 2861 2768
Size: 93 5164
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2201 2576 3318 3403 4474 1391
Size: 399 245 166 375 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 245 166 85
BspEI T'CCGG_A
Cuts at: 2397 4920 2397
Size: 2523 2734
BspHI T'CATG_A
Cuts at: 3146 4876 3146
Size: 1730 3527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2612 844
Size: 1768 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3144 1394
Size: 429 1321 3507
Fragments arranged by size:
3507 1321 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3752 3926 65
Size: 1183 2504 174 1396
Fragments arranged by size:
2504 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 3901 4088 1700
Size: 110 252 1839 187 2869
Fragments arranged by size:
2869 1839 252 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5257
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5257
BssSI C'ACGA_G
Cuts at: 2315 3317 4329 2315
Size: 1002 1012 3243
Fragments arranged by size:
3243 1012 1002
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5257
BstBI TT'CG_AA
Cuts at: 4061 4061
Size: 5257
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5257
BstXI CCAn_nnnn'nTGG
Cuts at: 2162 2551 2162
Size: 389 4868
BstYI r'GATC_y
Cuts at: 1972 2587 2954 3339 3356 4808 4894 4906 5252
Size: 615 367 385 17 1452 86 12 346
Cuts at: 5252 1972
Size: 1977
Fragments arranged by size:
1977 1452 615 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4705
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4518
Fragments arranged by size:
4518 683 56
BtsI GCAGTG_nn'
Cuts at: 2199 2534 2773 3567 3587 2199
Size: 335 239 794 20 3869
Fragments arranged by size:
3869 794 335 239 20
ClaI AT'CG_AT
Cuts at: 1554 2547 1554
Size: 993 4264
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5222
DraI TTT'AAA
Cuts at: 2561 2561
Size: 5257
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4264 809
Size: 507 2948 1802
Fragments arranged by size:
2948 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2362 2704 3593
Size: 54 1425 50 275 18 532 342 889
Cuts at: 3593 5222 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 532 342 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2704 1537
Size: 275 892 4090
Fragments arranged by size:
4090 892 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2501 3187 1353
Size: 1148 686 3423
Fragments arranged by size:
3423 1148 686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3844 4218 4364 387
Size: 1064 138 2255 374 146 1280
Fragments arranged by size:
2255 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2152 2246 2291 3320 3917 4704
Size: 393 459 61 94 45 1029 597 787
Cuts at: 4704 4972 1239
Size: 268 1524
Fragments arranged by size:
1524 1029 787 597 459 393 268 94
61 45
EcoO109I rG'GnC_Cy
Cuts at: 2052 2088 2242 2052
Size: 36 154 5067
Fragments arranged by size:
5067 154 36
EcoRI G'AATT_C
Cuts at: 2566 2944 2566
Size: 378 4879
FspI TGC'GCA
Cuts at: 3763 3763
Size: 5257
HaeII r_GCGC'y
Cuts at: 1678 4404 1678
Size: 2726 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3898 3930 3972 4004 1373
Size: 32 135 32 2326 32 42 32 2626
Fragments arranged by size:
2626 2326 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2601 2847 669
Size: 988 281 663 246 3079
Fragments arranged by size:
3079 988 663 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5257
HpaI GTT'AAC
Cuts at: 2847 2847
Size: 5257
KpnI G_GTAC'C
Cuts at: 2582 2582
Size: 5257
MfeI C'AATT_G
Cuts at: 2856 2856
Size: 5257
MluI A'CGCG_T
Cuts at: 5133 5133
Size: 5257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4371 4555 1305
Size: 3066 184 2007
Fragments arranged by size:
3066 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3435
Fragments arranged by size:
3435 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 2456 2519 3215 3574 3733 518
Size: 648 1038 252 63 696 359 159 2042
Fragments arranged by size:
2042 1038 696 648 359 252 159 63
MspA1I CmG'CkG
Cuts at: 1606 2673 3350 4498 4743 1606
Size: 1067 677 1148 245 2120
Fragments arranged by size:
2120 1148 1067 677 245
NaeI GCC'GGC
Cuts at: 2064 2064
Size: 5257
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4705
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5257
NgoMIV G'CCGG_C
Cuts at: 2062 2062
Size: 5257
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5257
NotI GC'GGCC_GC
Cuts at: 2704 2704
Size: 5257
NspI r_CATG'y
Cuts at: 1132 1229 2252 2615 4160 1132
Size: 97 1023 363 1545 2229
Fragments arranged by size:
2229 1545 1023 363 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5257
PciI A'CATG_T
Cuts at: 4156 4156
Size: 5257
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5257
PfoI T'CCnGG_A
Cuts at: 2168 2168
Size: 5257
PmeI GTTT'AAAC
Cuts at: 2561 2561
Size: 5257
PmlI CAC'GTG
Cuts at: 2519 2519
Size: 5257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 4839 4871 3326
Size: 32 1481 32 3712
Fragments arranged by size:
3712 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2052 2088 2052
Size: 36 5221
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5257
PsiI TTA'TAA
Cuts at: 2827 2827
Size: 5257
PstI C_TGCA'G
Cuts at: 830 2609 830
Size: 1779 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5257
SalI G'TCGA_C
Cuts at: 1936 2599 1936
Size: 663 4594
SanDI GG'GwC_CC
Cuts at: 2052 2088 2052
Size: 36 5221
SbfI CC_TGCA'GG
Cuts at: 2609 2609
Size: 5257
ScaI AGT'ACT
Cuts at: 3505 5217 3505
Size: 1712 3545
SfcI C'TryA_G
Cuts at: 826 1045 2486 2605 3740 4421 4612 826
Size: 219 1441 119 1135 681 191 1471
Fragments arranged by size:
1471 1441 1135 681 219 191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5257
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5257
SmaI CCC'GGG
Cuts at: 1872 2212 2584 1872
Size: 340 372 4545
Fragments arranged by size:
4545 372 340
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2300 3361 4262 4524 820
Size: 197 926 357 1061 901 262 1553
Fragments arranged by size:
1553 1061 926 901 357 262 197
SnaBI TAC'GTA
Cuts at: 493 3048 493
Size: 2555 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5257
SphI G_CATG'C
Cuts at: 1132 2615 1132
Size: 1483 3774
SspI AAT'ATT
Cuts at: 5 52 3181 5
Size: 47 3129 2081
Fragments arranged by size:
3129 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5257
StyI C'CwwG_G
Cuts at: 513 1065 2092 2188 2696 513
Size: 552 1027 96 508 3074
Fragments arranged by size:
3074 1027 552 508 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3284 3301 3454 3639 4084 325
Size: 1255 1704 17 153 185 445 1498
Fragments arranged by size:
1704 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3503 5215 96
Size: 274 80 33 51 1443 1526 1712 138
Fragments arranged by size:
1712 1526 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5217 5249 5217
Size: 32 5225
Tth111I GACn'n_nGTC
Cuts at: 1316 2146 1316
Size: 830 4427
XbaI T'CTAG_A
Cuts at: 2593 2593
Size: 5257
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5257
XmaI C'CCGG_G
Cuts at: 1870 2210 2582 1870
Size: 340 372 4545
Fragments arranged by size:
4545 372 340
XmnI GAAnn'nnTTC
Cuts at: 3386 3386
Size: 5257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3065 276
Size: 53 83 186 936 1531 2468
Fragments arranged by size:
2468 1531 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI