Restriction map for
pFN21AE3959
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BanI
BbeI BbrPI BbsI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FauI FbaI
FseI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MflI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AfaI 777 GTAC GT^AC
AflIII 800 ACATGT A^C[GA][CT]GT
AlwI 1067 GGATC GGATC(4/5)
AspHI 678 GAGCTC G[AT]GC[AT]^C
AvaI 661 CCCGAG C^[CT]CG[GA]G
AviII 438 TGCGCA TGC^GCA
BbvCI 58 GCTGAGG CCTCAGC(-5/-2)
BciVI 1028 GTATCC GTATCC(6/5)
BfaI 428 CTAG C^TAG
BsiHKAI 678 GAGCTC G[AT]GC[AT]^C
BsmI 989 GCATTC GAATGC(1/-1)
BsoBI 661 CCCGAG C^[CT]CG[GA]G
BspLU11I 800 ACATGT A^CATGT
BsrDI 1180 GCAATG GCAATG(2/0)
BstAPI 813 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1095 GGTCACC G^GT[ACGT]ACC
BstPI 1095 GGTCACC G^GT[ACGT]ACC
DrdI 91 GACCTTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1291 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1095 GGTCACC G^GT[ACGT]ACC
FspI 438 TGCGCA TGC^GCA
HphI 1097 TCACC GGTGA(8/7)
MaeI 428 CTAG C^TAG
MfeI 999 CAATTG C^AATTG
MslI 936 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 999 CAATTG C^AATTG
PflMI 1297 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 456 GACTC GAGTC(4/5)
RsaI 777 GTAC GT^AC
SacI 678 GAGCTC GAGCT^C
SapI 4 GAAGAGC GCTCTTC(1/4)
SbfI 1196 CCTGCAGG CCTGCA^GG
Sse8387I 1196 CCTGCAGG CCTGCA^GG
SspI 1502 AATATT AAT^ATT
TaqI 446 TCGA T^CGA
Tsp45I 1096 GTCAC ^GT[GC]AC
TthHB8I 446 TCGA T^CGA
Van91I 1297 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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ApaI 44 GGGCCC GGGCC^C
164 GGGCCC
AvaII 90 GGACC G^G[AT]CC
1467 GGACC
BalI 377 TGGCCA TGG^CCA
1409 TGGCCA
BsgI 907 GTGCAG GTGCAG(16/14)
1089 GTGCAG
BsmFI 1475 GGGAC GGGAC(10/14)
187 GTCCC
BssSI 31 CACGAG CACGAG(-5/-1)
877 CACGAG
CfoI 439 GCGC GCG^C
1316 GCGC
DpnII 294 GATC ^GATC
1068 GATC
EaeI 377 TGGCCA [CT]^GGCC[GA]
1409 TGGCCA
EarI 4 GAAGAG CTCTTC(1/4)
1144 GAAGAG
EcoT14I 783 CCATGG C^C[AT][AT]GG
827 CCATGG
HhaI 439 GCGC GCG^C
1316 GCGC
HinP1I 439 GCGC G^CGC
1316 GCGC
Ksp632I 4 GAAGAG CTCTTC(1/4)
1144 GAAGAG
MboI 294 GATC ^GATC
1068 GATC
MluNI 377 TGGCCA TGG^CCA
1409 TGGCCA
MscI 377 TGGCCA TGG^CCA
1409 TGGCCA
MseI 1382 TTAA T^TAA
1424 TTAA
MspA1I 273 CAGCGG C[AC]G^C[GT]G
642 CCGCTG
NcoI 783 CCATGG C^CATGG
827 CCATGG
NdeI 792 CATATG CA^TATG
853 CATATG
NdeII 294 GATC ^GATC
1068 GATC
Sau3AI 294 GATC ^GATC
1068 GATC
StyI 783 CCATGG C^C[AT][AT]GG
827 CCATGG
Tru9I 1382 TTAA T^TAA
1424 TTAA
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwNI AocI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I Cfr13I DdeI
DraII DsaI Eco81I EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeIII MboII MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV NspI PstI Sau96I ScrFI
SfaNI SfcI StuI TfiI TseI
Tsp509I TspRI