Restriction map for
pFN21AE3959
(Circular) (Six-base) MAPSORT of: pFN21AE3959.seq Check: 77 from: 1 to: 6226
pFN21AE3959 6226 bp
With 182 enzymes: SgfI *
March 28, 2012 11:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2048 2411 2579 3030 2048
Size: 363 168 451 5244
Fragments arranged by size:
5244 451 363 168
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4036 278
Size: 53 83 186 936 2500 2468
Fragments arranged by size:
2500 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3569 1937
Size: 1632 4594
Acc65I G'GTAC_C
Cuts at: 3547 3547
Size: 6226
AclI AA'CG_TT
Cuts at: 1563 4353 4726 1563
Size: 2790 373 3063
Fragments arranged by size:
3063 2790 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4289 5673 5941 1632
Size: 2657 1384 268 1917
Fragments arranged by size:
2657 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6029
AflIII A'CryG_T
Cuts at: 1203 2805 5125 6102 1203
Size: 1602 2320 977 1327
Fragments arranged by size:
2320 1602 1327 977
AgeI A'CCGG_T
Cuts at: 5057 5057
Size: 6226
AhdI GACnn_n'nnGTC
Cuts at: 4955 4955
Size: 6226
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2741 1184
Size: 1557 4669
AlwNI CAG_nnn'CTG
Cuts at: 2063 2195 2693 3969 5541 2063
Size: 132 498 1276 1572 2748
Fragments arranged by size:
2748 1572 1276 498 132
ApaI G_GGCC'C
Cuts at: 2053 2173 2053
Size: 120 6106
ApaLI G'TGCA_C
Cuts at: 4283 5439 4283
Size: 1156 5070
ApoI r'AATT_y
Cuts at: 1493 3535 3761 3913 6022 1493
Size: 2042 226 152 2109 1697
Fragments arranged by size:
2109 2042 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4780 160
Size: 872 3748 1606
Fragments arranged by size:
3748 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
AvaI C'yCGr_G
Cuts at: 1870 1943 2666 3551 1870
Size: 73 723 885 4545
Fragments arranged by size:
4545 885 723 73
BamHI G'GATC_C
Cuts at: 3556 3923 3556
Size: 367 5859
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3547 5002 618
Size: 325 217 627 1760 1455 1842
Fragments arranged by size:
1842 1760 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2053 2115 2173 2457 2687 3545 1391
Size: 662 62 58 284 230 858 4072
Fragments arranged by size:
4072 858 662 284 230 62 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5255
BbvCI CC'TCA_GC
Cuts at: 2064 2064
Size: 6226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4417 4451 1903
Size: 34 2480 34 3678
Fragments arranged by size:
3678 2480 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3044 4118 5328 3044
Size: 1074 1210 3942
Fragments arranged by size:
3942 1210 1074
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6226
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2486 2669
Size: 107 122 71 631 605 367 447 183
Cuts at: 2669 2991 3298 4837 136
Size: 322 307 1539 1525
Fragments arranged by size:
1539 1525 631 605 447 367 322 307
183 122 107 71
BglII A'GATC_T
Cuts at: 6221 6221
Size: 6226
BlpI GC'TnA_GC
Cuts at: 3643 3643
Size: 6226
Bme1580I G_kGCm'C
Cuts at: 1790 2053 2173 4287 5443 1790
Size: 263 120 2114 1156 2573
Fragments arranged by size:
2573 2114 1156 263 120
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6226
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2250 3117 3957
Size: 416 53 83 399 24 833 867 840
Cuts at: 3957 4915 442
Size: 958 1753
Fragments arranged by size:
1753 958 867 840 833 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2118 2172 2601 4886 1239
Size: 879 54 429 2285 2579
Fragments arranged by size:
2579 2285 879 429 54
Bpu10I CC'TnA_GC
Cuts at: 2064 2064
Size: 6226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4351 5216 5514 4351
Size: 865 298 5063
Fragments arranged by size:
5063 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4889 882
Size: 617 3390 2219
Fragments arranged by size:
3390 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4017 493
Size: 711 2813 2702
Fragments arranged by size:
2813 2702 711
BsaBI GATnn'nnATC
Cuts at: 6220 6220
Size: 6226
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4033 4415 5050 275
Size: 53 83 186 936 2500 382 635 1451
Fragments arranged by size:
2500 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4659 5057 5331 5478 5889 4659
Size: 398 274 147 411 4996
Fragments arranged by size:
4996 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2069 2099 2577 2607 1178
Size: 30 165 30 666 30 478 30 4797
Fragments arranged by size:
4797 666 478 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2226 2256 2259 2598 2664 3155 3158 1186
Size: 1040 30 3 339 66 491 3 4254
Fragments arranged by size:
4254 1040 491 339 66 30 3 3
BseYI C'CCAG_C
Cuts at: 2166 2230 2601 2649 3279 5429 5925 2166
Size: 64 371 48 630 2150 496 2467
Fragments arranged by size:
2467 2150 630 496 371 64 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2933 3115 1163
Size: 573 1197 182 4274
Fragments arranged by size:
4274 1197 573 182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3676 4437 5465 1540
Size: 275 186 1675 761 1028 2301
Fragments arranged by size:
2301 1675 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2687 4287 4372 5443 2687
Size: 1600 85 1071 3470
Fragments arranged by size:
3470 1600 1071 85
BsmI GAATG_Cn'
Cuts at: 2994 3737 3830 2994
Size: 743 93 5390
Fragments arranged by size:
5390 743 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2115 2173 2457 2687 3545 4287
Size: 399 263 62 58 284 230 858 742
Cuts at: 4287 4372 5443 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 858 742 399 284 263 230
85 62 58
BspEI T'CCGG_A
Cuts at: 5889 5889
Size: 6226
BspHI T'CATG_A
Cuts at: 4115 5845 4115
Size: 1730 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2048 2298 2411 2579 3030 3195 3581 844
Size: 1204 250 113 168 451 165 386 3489
Fragments arranged by size:
3489 1204 451 386 250 168 165 113
BsrBI CCG'CTC
Cuts at: 1394 1823 4113 1394
Size: 429 2290 3507
Fragments arranged by size:
3507 2290 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3192 4721 4895 65
Size: 1183 1944 1529 174 1396
Fragments arranged by size:
1944 1529 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4870 5057 1700
Size: 110 3060 187 2869
Fragments arranged by size:
3060 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6226
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6226
BssSI C'ACGA_G
Cuts at: 2036 2882 4286 5298 2036
Size: 846 1404 1012 2964
Fragments arranged by size:
2964 1404 1012 846
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2824 794
Size: 2030 4196
BstBI TT'CG_AA
Cuts at: 5030 5030
Size: 6226
BstEII G'GTnAC_C
Cuts at: 1184 3100 1184
Size: 1916 4310
BstYI r'GATC_y
Cuts at: 1972 3556 3923 4308 4325 5777 5863 5875 6221
Size: 1584 367 385 17 1452 86 12 346
Cuts at: 6221 1972
Size: 1977
Fragments arranged by size:
1977 1584 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2219 2236 3273 2219
Size: 17 1037 5172
Fragments arranged by size:
5172 1037 17
BtgI C'CryG_G
Cuts at: 513 1065 2004 2569 2788 2832 513
Size: 552 939 565 219 44 3907
Fragments arranged by size:
3907 939 565 552 219 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5487
Fragments arranged by size:
5487 683 56
BtsI GCAGTG_nn'
Cuts at: 2333 2725 3742 4536 4556 2333
Size: 392 1017 794 20 4003
Fragments arranged by size:
4003 1017 794 392 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2917 2952 529
Size: 35 2353 35 3803
Fragments arranged by size:
3803 2353 35 35
DraI TTT'AAA
Cuts at: 3530 3530
Size: 6226
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2102 5233 809
Size: 507 786 3131 1802
Fragments arranged by size:
3131 1802 786 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 3414 3673
Size: 54 1425 50 275 18 552 1032 259
Cuts at: 3673 4562 6191 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1032 889 552 275 259 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3673 1537
Size: 275 1861 4090
Fragments arranged by size:
4090 1861 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2004 3144 4156 1353
Size: 651 1140 1012 3423
Fragments arranged by size:
3423 1140 1012 651
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2489 2621 4813 5187 5333 387
Size: 1064 138 900 132 2192 374 146 1280
Fragments arranged by size:
2192 1280 1064 900 374 146 138 132
EcoICRI GAG'CTC
Cuts at: 2685 2685
Size: 6226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2172 2601 4289 4886 5673 5941
Size: 393 486 54 429 1688 597 787 268
Cuts at: 5941 1239
Size: 1524
Fragments arranged by size:
1688 1524 787 597 486 429 393 268
54
EcoNI CCTnn'n_nnAGG
Cuts at: 3300 3300
Size: 6226
EcoO109I rG'GnC_Cy
Cuts at: 2049 2170 2412 2429 2865 2049
Size: 121 242 17 436 5410
Fragments arranged by size:
5410 436 242 121 17
EcoRI G'AATT_C
Cuts at: 3535 3913 3535
Size: 378 5848
FspI TGC'GCA
Cuts at: 2445 4732 2445
Size: 2287 3939
HaeII r_GCGC'y
Cuts at: 1678 5373 1678
Size: 3695 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 4867 4899 4941
Size: 32 135 32 497 32 2766 32 42
Cuts at: 4941 4973 1373
Size: 32 2626
Fragments arranged by size:
2766 2626 497 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3570 3816 669
Size: 988 281 1632 246 3079
Fragments arranged by size:
3079 1632 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6226
HpaI GTT'AAC
Cuts at: 3816 3816
Size: 6226
KpnI G_GTAC'C
Cuts at: 3551 3551
Size: 6226
MfeI C'AATT_G
Cuts at: 3004 3825 3004
Size: 821 5405
MluI A'CGCG_T
Cuts at: 6102 6102
Size: 6226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2354 5340 5524 1305
Size: 1049 2986 184 2007
Fragments arranged by size:
2986 2007 1049 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 3416 10
Size: 54 1425 343 552 1032 2820
Fragments arranged by size:
2820 1425 1032 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2945 4184 4543 4702 518
Size: 648 1779 1239 359 159 2042
Fragments arranged by size:
2042 1779 1239 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2280 2649 3642 4319 5467 5712 1606
Size: 674 369 993 677 1148 245 2120
Fragments arranged by size:
2120 1148 993 677 674 369 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2788 2832 513
Size: 552 939 784 44 3907
Fragments arranged by size:
3907 939 784 552 44
NdeI CA'TA_TG
Cuts at: 387 2798 2859 387
Size: 2411 61 3754
Fragments arranged by size:
3754 2411 61
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6226
NotI GC'GGCC_GC
Cuts at: 3673 3673
Size: 6226
NspI r_CATG'y
Cuts at: 1132 1229 2156 2809 3252 3373 3584 5129 1132
Size: 97 927 653 443 121 211 1545 2229
Fragments arranged by size:
2229 1545 927 653 443 211 121 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6226
PciI A'CATG_T
Cuts at: 2805 5125 2805
Size: 2320 3906
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3308 1108
Size: 2200 4026
PmeI GTTT'AAAC
Cuts at: 3530 3530
Size: 6226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 5808 5840 4295
Size: 32 1481 32 4681
Fragments arranged by size:
4681 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6226
PsiI TTA'TAA
Cuts at: 3796 3796
Size: 6226
PspOMI G'GGCC_C
Cuts at: 2049 2169 2049
Size: 120 6106
PstI C_TGCA'G
Cuts at: 830 2020 2309 2422 2820 3206 3578 830
Size: 1190 289 113 398 386 372 3478
Fragments arranged by size:
3478 1190 398 386 372 289 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6226
SacI G_AGCT'C
Cuts at: 2687 2687
Size: 6226
SalI G'TCGA_C
Cuts at: 1936 3568 1936
Size: 1632 4594
SapI GCTCTTCn'nnn_
Cuts at: 2004 2004
Size: 6226
SbfI CC_TGCA'GG
Cuts at: 3206 3578 3206
Size: 372 5854
ScaI AGT'ACT
Cuts at: 4474 6186 4474
Size: 1712 4514
SfcI C'TryA_G
Cuts at: 826 1045 2016 2181 2305 2418 2678 2816 3202
Size: 219 971 165 124 113 260 138 386
Cuts at: 3202 3574 4709 5390 5581 826
Size: 372 1135 681 191 1471
Fragments arranged by size:
1471 1135 971 681 386 372 260 219
191 165 138 124 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6226
SmaI CCC'GGG
Cuts at: 1872 3553 1872
Size: 1681 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4330 5231 5493 820
Size: 197 926 2387 901 262 1553
Fragments arranged by size:
2387 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4017 493
Size: 3524 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6226
SphI G_CATG'C
Cuts at: 1132 3584 1132
Size: 2452 3774
SspI AAT'ATT
Cuts at: 5 52 3509 4150 5
Size: 47 3457 641 2081
Fragments arranged by size:
3457 2081 641 47
StuI AGG'CCT
Cuts at: 1517 2217 2224 3200 1517
Size: 700 7 976 4543
Fragments arranged by size:
4543 976 700 7
StyI C'CwwG_G
Cuts at: 513 1065 2004 2788 2832 3665 513
Size: 552 939 784 44 833 3074
Fragments arranged by size:
3074 939 833 784 552 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3124 4253 4270 4423 4608 5053 325
Size: 1255 1544 1129 17 153 185 445 1498
Fragments arranged by size:
1544 1498 1255 1129 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4472 6184 96
Size: 274 80 33 51 1443 2495 1712 138
Fragments arranged by size:
2495 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2918 2950 6186 6218 2918
Size: 32 3236 32 2926
Fragments arranged by size:
3236 2926 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6226
XbaI T'CTAG_A
Cuts at: 3562 3562
Size: 6226
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6226
XmaI C'CCGG_G
Cuts at: 1870 3551 1870
Size: 1681 4545
XmnI GAAnn'nnTTC
Cuts at: 4355 4355
Size: 6226
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4034 276
Size: 53 83 186 936 2500 2468
Fragments arranged by size:
2500 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI