(Linear) (Six-base) MAPSORT of: KIEE3959.seq Check: 6479 from: 1 to: 1521 KIEE3959.orf With 182 enzymes: SgfI * March 22, 2012 16:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 43 406 574 1025 1521 Size: 43 363 168 451 496 Fragments arranged by size: 496 451 363 168 43 AflIII A'CryG_T Cuts at: 0 800 1521 Size: 800 721 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 736 1521 Size: 736 785 AlwNI CAG_nnn'CTG Cuts at: 0 58 190 688 1521 Size: 58 132 498 833 Fragments arranged by size: 833 498 132 58 ApaI G_GGCC'C Cuts at: 0 48 168 1521 Size: 48 120 1353 Fragments arranged by size: 1353 120 48 AvaI C'yCGr_G Cuts at: 0 661 1521 Size: 661 860 BanII G_rGCy'C Cuts at: 0 48 110 168 452 682 1521 Size: 48 62 58 284 230 839 Fragments arranged by size: 839 284 230 62 58 48 BbvCI CC'TCA_GC Cuts at: 0 59 1521 Size: 59 1462 BciVI GTATCCnnnnn_n' Cuts at: 0 1039 1521 Size: 1039 482 BglI GCCn_nnn'nGGC Cuts at: 0 34 481 664 986 1293 1521 Size: 34 447 183 322 307 228 Fragments arranged by size: 447 322 307 228 183 34 Bme1580I G_kGCm'C Cuts at: 0 48 168 1521 Size: 48 120 1353 Fragments arranged by size: 1353 120 48 BmrI ACTGGGnnnn_n' Cuts at: 0 245 1112 1521 Size: 245 867 409 Fragments arranged by size: 867 409 245 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 113 167 596 1521 Size: 113 54 429 925 Fragments arranged by size: 925 429 113 54 Bpu10I CC'TnA_GC Cuts at: 0 59 1521 Size: 59 1462 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 64 94 572 602 1521 Size: 64 30 478 30 919 Fragments arranged by size: 919 478 64 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 221 251 254 593 659 1150 1153 1521 Size: 221 30 3 339 66 491 3 368 Fragments arranged by size: 491 368 339 221 66 30 3 3 BseYI C'CCAG_C Cuts at: 0 161 225 596 644 1274 1521 Size: 161 64 371 48 630 247 Fragments arranged by size: 630 371 247 161 64 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 928 1110 1521 Size: 928 182 411 Fragments arranged by size: 928 411 182 BsiHKAI G_wGCw'C Cuts at: 0 682 1521 Size: 682 839 BsmI GAATG_Cn' Cuts at: 0 989 1521 Size: 989 532 Bsp1286I G_dGCh'C Cuts at: 0 48 110 168 452 682 1521 Size: 48 62 58 284 230 839 Fragments arranged by size: 839 284 230 62 58 48 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 43 293 406 574 1025 1190 1521 Size: 43 250 113 168 451 165 331 Fragments arranged by size: 451 331 250 168 165 113 43 BsrDI GCAATG_nn' Cuts at: 0 1187 1521 Size: 1187 334 BssSI C'ACGA_G Cuts at: 0 31 877 1521 Size: 31 846 644 Fragments arranged by size: 846 644 31 BstAPI GCAn_nnn'nTGC Cuts at: 0 819 1521 Size: 819 702 BstEII G'GTnAC_C Cuts at: 0 1095 1521 Size: 1095 426 Bsu36I CC'TnA_GG Cuts at: 0 214 231 1268 1521 Size: 214 17 1037 253 Fragments arranged by size: 1037 253 214 17 BtgI C'CryG_G Cuts at: 0 564 783 827 1521 Size: 564 219 44 694 Fragments arranged by size: 694 564 219 44 BtsI GCAGTG_nn' Cuts at: 0 328 720 1521 Size: 328 392 801 Fragments arranged by size: 801 392 328 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 912 947 1521 Size: 912 35 574 Fragments arranged by size: 912 574 35 DrdI GACnn_nn'nnGTC Cuts at: 0 97 1521 Size: 97 1424 EaeI y'GGCC_r Cuts at: 0 377 1409 1521 Size: 377 1032 112 Fragments arranged by size: 1032 377 112 EarI CTCTTCn'nnn_ Cuts at: 0 1139 1521 Size: 1139 382 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 484 616 1521 Size: 484 132 905 Fragments arranged by size: 905 484 132 EcoICRI GAG'CTC Cuts at: 0 680 1521 Size: 680 841 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 113 167 596 1521 Size: 113 54 429 925 Fragments arranged by size: 925 429 113 54 EcoNI CCTnn'n_nnAGG Cuts at: 0 1295 1521 Size: 1295 226 EcoO109I rG'GnC_Cy Cuts at: 0 44 165 407 424 860 1521 Size: 44 121 242 17 436 661 Fragments arranged by size: 661 436 242 121 44 17 FspI TGC'GCA Cuts at: 0 440 1521 Size: 440 1081 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 64 96 1521 Size: 64 32 1425 Fragments arranged by size: 1425 64 32 MfeI C'AATT_G Cuts at: 0 999 1521 Size: 999 522 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 349 1521 Size: 349 1172 MscI TGG'CCA Cuts at: 0 379 1411 1521 Size: 379 1032 110 Fragments arranged by size: 1032 379 110 MslI CAynn'nnrTG Cuts at: 0 940 1521 Size: 940 581 MspA1I CmG'CkG Cuts at: 0 275 644 1521 Size: 275 369 877 Fragments arranged by size: 877 369 275 NcoI C'CATG_G Cuts at: 0 783 827 1521 Size: 783 44 694 Fragments arranged by size: 783 694 44 NdeI CA'TA_TG Cuts at: 0 793 854 1521 Size: 793 61 667 Fragments arranged by size: 793 667 61 NspI r_CATG'y Cuts at: 0 151 804 1247 1368 1521 Size: 151 653 443 121 153 Fragments arranged by size: 653 443 153 151 121 PciI A'CATG_T Cuts at: 0 800 1521 Size: 800 721 PflMI CCAn_nnn'nTGG Cuts at: 0 1303 1521 Size: 1303 218 PspOMI G'GGCC_C Cuts at: 0 44 164 1521 Size: 44 120 1357 Fragments arranged by size: 1357 120 44 PstI C_TGCA'G Cuts at: 0 15 304 417 815 1201 1521 Size: 15 289 113 398 386 320 Fragments arranged by size: 398 386 320 289 113 15 SacI G_AGCT'C Cuts at: 0 682 1521 Size: 682 839 SbfI CC_TGCA'GG Cuts at: 0 1201 1521 Size: 1201 320 SfcI C'TryA_G Cuts at: 0 11 176 300 413 673 811 1197 1521 Size: 11 165 124 113 260 138 386 324 Fragments arranged by size: 386 324 260 165 138 124 113 11 SspI AAT'ATT Cuts at: 0 1504 1521 Size: 1504 17 StuI AGG'CCT Cuts at: 0 212 219 1195 1521 Size: 212 7 976 326 Fragments arranged by size: 976 326 212 7 StyI C'CwwG_G Cuts at: 0 783 827 1521 Size: 783 44 694 Fragments arranged by size: 783 694 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1119 1521 Size: 1119 402 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 913 945 1521 Size: 913 32 576 Fragments arranged by size: 913 576 32 Enzymes that do cut: AarI AflIII AlfI AlwNI ApaI AvaI BanII BbvCI BciVI BglI Bme1580I BmrI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstAPI BstEII Bsu36I BtgI BtsI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI PciI PflMI PspOMI PstI SacI SbfI SfcI SspI StuI StyI TaqII TstI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstXI BstYI BstZ17I BtgZI ClaI DraI DraIII EagI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI