################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AccI AclI AhdI AocI AseI AsnI AspEI AvrII BamHI BbeI BlnI BlpI Bpu1102I BsiWI Bsp1407I BspDI BspHI BsrGI BssSI Bst1107I BstZ17I Bsu36I CelII ClaI CpoI DraIII Eam1105I Eco81I EcoRV HincII HindII HpaI KasI MfeI MluI MunI NarI NdeI NheI NotI NruI PacI PaeR7I PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SfoI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 3226 GACGTC GACGT^C Acc65I 2042 GGTACC G^GTACC AccIII 3383 TCCGGA T^CCGGA AflII 819 CTTAAG C^TTAAG AflIII 1842 ACATGT A^C[GA][CT]GT Alw44I 3160 GTGCAC G^TGCAC Aor51HI 524 AGCGCT AGC^GCT ApaI 1133 GGGCCC GGGCC^C ApaLI 3160 GTGCAC G^TGCAC AscI 79 GGCGCGCC GG^CGCGCC Asp718I 2042 GGTACC G^GTACC AspI 2842 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3796 CCCGAG C^[CT]CG[GA]G AviII 3936 TGCGCA TGC^GCA BalI 1649 TGGCCA TGG^CCA BbrPI 3175 CACGTG CAC^GTG BciVI 2743 GGATAC GTATCC(6/5) BfrI 819 CTTAAG C^TTAAG BsaAI 3175 CACGTG [CT]AC^GT[GA] BsaBI 2968 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3048 GAGACC GGTCTC(1/5) BseAI 3383 TCCGGA T^CCGGA BsmI 3581 GAATGC GAATGC(1/-1) BsoBI 3796 CCCGAG C^[CT]CG[GA]G BspEI 3383 TCCGGA T^CCGGA BspLU11I 1842 ACATGT A^CATGT BsrBI 3123 GAGCGG CCGCTC(-3/-3) BssHII 80 GCGCGC G^CGCGC BstAPI 2857 GCACCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 208 TTCGAA TT^CGAA BstXI 3410 CCAATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 2390 GACAGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 524 AGCGCT AGC^GCT EcoNI 3138 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 194 GGCCGGCC GGCCGG^CC FspI 3936 TGCGCA TGC^GCA KpnI 2042 GGTACC GGTAC^C KspI 155 CCGCGG CCGC^GG MamI 2968 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1649 TGGCCA TGG^CCA MroI 3383 TCCGGA T^CCGGA MscI 1649 TGGCCA TGG^CCA NaeI 195 GCCGGC GCC^GGC NcoI 459 CCATGG C^CATGG NgoMIV 195 GCCGGC G^CCGGC NspV 208 TTCGAA TT^CGAA PflFI 2842 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3175 CACGTG CAC^GTG PmlI 3175 CACGTG CAC^GTG SacII 155 CCGCGG CCGC^GG SexAI 638 ACCAGGT A^CC[AT]GGT SfiI 189 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 208 TTCGAA TT^CGAA SgrAI 3698 CACCGGTG C[GA]^CCGG[CT]G Tth111I 2842 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 81 CGCG CG^CG 156 CGCG AcyI 3226 GACGTC G[GA]^CG[CT]C 3556 GACGCC AgeI 1035 ACCGGT A^CCGGT 3699 ACCGGT AlwNI 1301 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1432 CAGAGTCTG BbvCI 3681 CCTCAGC CCTCAGC(-5/-2) 2926 GCTGAGG BcgI 1637 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3686 GCATGTTATTCG BclI 1557 TGATCA T^GATCA 3720 TGATCA BglII 2688 AGATCT A^GATCT 2967 AGATCT BsaHI 3226 GACGTC G[GA]^CG[CT]C 3556 GACGCC BsmBI 2583 CGTCTC CGTCTC(1/5) 3224 GAGACG BspMI 1239 GCAGGT ACCTGC(4/8) 2233 GCAGGT BsrDI 3951 CATTGC GCAATG(2/0) 1909 GCAATG BstEII 57 GGTGACC G^GT[ACGT]ACC 549 GGTCACC BstPI 57 GGTGACC G^GT[ACGT]ACC 549 GGTCACC BstUI 81 CGCG CG^CG 156 CGCG DraI 2002 TTTAAA TTT^AAA 2470 TTTAAA EagI 193 CGGCCG C^GGCCG 197 CGGCCG EclXI 193 CGGCCG C^GGCCG 197 CGGCCG Eco52I 193 CGGCCG C^GGCCG 197 CGGCCG EcoO65I 57 GGTGACC G^GT[ACGT]ACC 549 GGTCACC EcoRI 739 GAATTC G^AATTC 2443 GAATTC EcoT22I 1201 ATGCAT ATGCA^T 2155 ATGCAT FbaI 1557 TGATCA T^GATCA 3720 TGATCA Hin1I 3226 GACGTC G[GA]^CG[CT]C 3556 GACGCC HindIII 2782 AAGCTT A^AGCTT 3964 AAGCTT MvnI 81 CGCG CG^CG 156 CGCG NsiI 1201 ATGCAT ATGCA^T 2155 ATGCAT PinAI 1035 ACCGGT A^CCGGT 3699 ACCGGT PpuMI 2309 AGGACCC [GA]G^G[AT]CC[CT] 2671 AGGTCCC PshAI 1493 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2319 GACAACTGTC PvuII 870 CAGCTG CAG^CTG 1464 CAGCTG ScaI 3191 AGTACT AGT^ACT 3734 AGTACT SspI 1336 AATATT AAT^ATT 3859 AATATT XbaI 1501 TCTAGA T^CTAGA 1857 TCTAGA XcmI 303 CCAACCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 725 CCAAGAGTTTGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI