(Circular) (Six-base) MAPSORT of: pFN21AE4279.seq Check: 4696 from: 1 to: 8851 pFN21AE4279 8851 bp With 182 enzymes: SgfI * March 28, 2012 13:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4229 4229 Size: 8851 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5235 6661 278 Size: 53 83 186 936 3699 1426 2468 Fragments arranged by size: 3699 2468 1426 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6194 1937 Size: 4257 4594 Acc65I G'GTAC_C Cuts at: 4047 6172 4047 Size: 2125 6726 AclI AA'CG_TT Cuts at: 1563 6978 7351 1563 Size: 5415 373 3063 Fragments arranged by size: 5415 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2696 5589 6914 8298 8566 1632 Size: 1064 2893 1325 1384 268 1917 Fragments arranged by size: 2893 1917 1384 1325 1064 268 AfeI AGC'GCT Cuts at: 2531 2531 Size: 8851 AflII C'TTAA_G Cuts at: 820 1017 2824 820 Size: 197 1807 6847 Fragments arranged by size: 6847 1807 197 AflIII A'CryG_T Cuts at: 1203 3847 7750 8727 1203 Size: 2644 3903 977 1327 Fragments arranged by size: 3903 2644 1327 977 AgeI A'CCGG_T Cuts at: 3040 5704 7682 3040 Size: 2664 1978 4209 Fragments arranged by size: 4209 2664 1978 AhdI GACnn_n'nnGTC Cuts at: 7580 7580 Size: 8851 AleI CACnn'nnGTG Cuts at: 1166 4610 4721 5091 1166 Size: 3444 111 370 4926 Fragments arranged by size: 4926 3444 370 111 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5268 1184 Size: 4084 4767 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5004 5036 5004 Size: 32 8819 AlwNI CAG_nnn'CTG Cuts at: 3311 3442 6594 8166 3311 Size: 131 3152 1572 3996 Fragments arranged by size: 3996 3152 1572 131 ApaI G_GGCC'C Cuts at: 3142 3142 Size: 8851 ApaLI G'TGCA_C Cuts at: 5165 6908 8064 5165 Size: 1743 1156 5952 Fragments arranged by size: 5952 1743 1156 ApoI r'AATT_y Cuts at: 1493 2744 2833 3904 3958 4448 4924 5033 5507 Size: 1251 89 1071 54 490 476 109 474 Cuts at: 5507 6160 6386 6538 8647 1493 Size: 653 226 152 2109 1697 Fragments arranged by size: 2109 1697 1251 1071 653 490 476 474 226 152 109 89 54 AscI GG'CGCG_CC Cuts at: 2085 2085 Size: 8851 AseI AT'TA_AT Cuts at: 160 1032 7405 160 Size: 872 6373 1606 Fragments arranged by size: 6373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8851 AvaI C'yCGr_G Cuts at: 1870 1943 5801 6176 1870 Size: 73 3858 375 4545 Fragments arranged by size: 4545 3858 375 73 BamHI G'GATC_C Cuts at: 6181 6548 6181 Size: 367 8484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4047 4819 4861 6146 6172 Size: 325 217 627 2260 772 42 1285 26 Cuts at: 6172 7627 618 Size: 1455 1842 Fragments arranged by size: 2260 1842 1455 1285 772 627 325 217 42 26 BanII G_rGCy'C Cuts at: 1391 2240 3142 3649 4903 5262 6170 1391 Size: 849 902 507 1254 359 908 4072 Fragments arranged by size: 4072 1254 908 902 849 507 359 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4142 5146 5577 928 Size: 971 2243 1004 431 4202 Fragments arranged by size: 4202 2243 1004 971 431 BbvCI CC'TCA_GC Cuts at: 4932 5687 4932 Size: 755 8096 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3631 3665 5680 5714 7042 Size: 34 50 34 1610 34 2015 34 1328 Cuts at: 7042 7076 1903 Size: 34 3678 Fragments arranged by size: 3678 2015 1610 1328 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4742 6743 7953 4742 Size: 2001 1210 5640 Fragments arranged by size: 5640 2001 1210 BclI T'GATC_A Cuts at: 1548 3562 5725 1548 Size: 2014 2163 4674 Fragments arranged by size: 4674 2163 2014 BfrBI ATG'CAT Cuts at: 3208 4162 3208 Size: 954 7897 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 2201 3925 Size: 107 122 71 631 605 430 99 1724 Cuts at: 3925 7462 136 Size: 3537 1525 Fragments arranged by size: 3537 1724 1525 631 605 430 122 107 99 71 BglII A'GATC_T Cuts at: 4693 4972 8846 4693 Size: 279 3874 4698 Fragments arranged by size: 4698 3874 279 BlpI GC'TnA_GC Cuts at: 6268 6268 Size: 8851 Bme1580I G_kGCm'C Cuts at: 1790 3142 4824 5169 5355 6912 8068 1790 Size: 1352 1682 345 186 1557 1156 2573 Fragments arranged by size: 2573 1682 1557 1352 1156 345 186 BmgBI CAC'GTC Cuts at: 1318 2140 2364 1318 Size: 822 224 7805 Fragments arranged by size: 7805 822 224 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3136 5432 6582 Size: 416 53 83 399 24 1719 2296 1150 Cuts at: 6582 7540 442 Size: 958 1753 Fragments arranged by size: 2296 1753 1719 1150 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8851 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5252 5252 Size: 8851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2721 3053 5110 5769 7511 1239 Size: 1482 332 2057 659 1742 2579 Fragments arranged by size: 2579 2057 1742 1482 659 332 Bpu10I CC'TnA_GC Cuts at: 4598 4932 5687 4598 Size: 334 755 7762 Fragments arranged by size: 7762 755 334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4887 5288 6976 7841 8139 4887 Size: 401 1688 865 298 5599 Fragments arranged by size: 5599 1688 865 401 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5047 7514 882 Size: 617 3548 2467 2219 Fragments arranged by size: 3548 2467 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5182 6642 493 Size: 711 3978 1460 2702 Fragments arranged by size: 3978 2702 1460 711 BsaBI GATnn'nnATC Cuts at: 4977 8845 4977 Size: 3868 4983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5232 5562 6658 7040 Size: 53 83 186 936 3699 330 1096 382 Cuts at: 7040 7675 275 Size: 635 1451 Fragments arranged by size: 3699 1451 1096 936 635 382 330 186 83 53 BsaWI w'CCGG_w Cuts at: 3040 5388 5704 7284 7682 7956 8103 8514 3040 Size: 2348 316 1580 398 274 147 411 3377 Fragments arranged by size: 3377 2348 1580 411 398 316 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2619 2649 4031 4061 5807 Size: 30 165 30 1216 30 1382 30 1746 Cuts at: 5807 5837 1178 Size: 30 4192 Fragments arranged by size: 4192 1746 1382 1216 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 3012 4298 5282 5675 1186 Size: 949 877 1286 984 393 4362 Fragments arranged by size: 4362 1286 984 949 877 393 BseYI C'CCAG_C Cuts at: 2080 2559 8054 8550 2080 Size: 479 5495 496 2381 Fragments arranged by size: 5495 2381 496 479 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 5119 5162 1163 Size: 573 411 2972 43 4852 Fragments arranged by size: 4852 2972 573 411 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2201 2205 6085 6301 7062 8090 Size: 275 186 200 4 3880 216 761 1028 Cuts at: 8090 1540 Size: 2301 Fragments arranged by size: 3880 2301 1028 761 275 216 200 186 4 BsiHKAI G_wGCw'C Cuts at: 2240 3000 3649 3727 4903 5169 5262 5808 6144 Size: 760 649 78 1176 266 93 546 336 Cuts at: 6144 6912 6997 8068 2240 Size: 768 85 1071 3023 Fragments arranged by size: 3023 1176 1071 768 760 649 546 336 266 93 85 78 BsmI GAATG_Cn' Cuts at: 5592 6362 6455 5592 Size: 770 93 7988 Fragments arranged by size: 7988 770 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4594 5223 4594 Size: 629 8222 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 3000 3142 3649 3727 4824 4903 Size: 399 450 760 142 507 78 1097 79 Cuts at: 4903 5169 5262 5355 5808 6144 6170 6912 6997 Size: 266 93 93 453 336 26 742 85 Cuts at: 6997 8068 1391 Size: 1071 2174 Fragments arranged by size: 2174 1097 1071 760 742 507 453 450 399 336 266 142 93 93 85 79 78 26 BspEI T'CCGG_A Cuts at: 5388 8514 5388 Size: 3126 5725 BspHI T'CATG_A Cuts at: 6740 8470 6740 Size: 1730 7121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3235 4229 6206 844 Size: 2391 994 1977 3489 Fragments arranged by size: 3489 2391 1977 994 BsrBI CCG'CTC Cuts at: 1394 1823 5130 6738 1394 Size: 429 3307 1608 3507 Fragments arranged by size: 3507 3307 1608 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3921 5955 7346 7520 65 Size: 1183 2673 2034 1391 174 1396 Fragments arranged by size: 2673 2034 1396 1391 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2200 3040 3522 5704 7495 7682 1700 Size: 110 390 840 482 2182 1791 187 2869 Fragments arranged by size: 2869 2182 1791 840 482 390 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8851 BssHII G'CGCG_C Cuts at: 1925 2085 1925 Size: 160 8691 BssSI C'ACGA_G Cuts at: 6911 7923 6911 Size: 1012 7839 BstAPI GCAn_nnn'nTGC Cuts at: 794 4868 794 Size: 4074 4777 BstBI TT'CG_AA Cuts at: 2214 7655 2214 Size: 5441 3410 BstEII G'GTnAC_C Cuts at: 1184 2062 2554 1184 Size: 878 492 7481 Fragments arranged by size: 7481 878 492 BstXI CCAn_nnnn'nTGG Cuts at: 5422 5422 Size: 8851 BstYI r'GATC_y Cuts at: 1972 4642 4693 4972 5624 6181 6548 6933 6950 Size: 2670 51 279 652 557 367 385 17 Cuts at: 6950 8402 8488 8500 8846 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2670 1977 1452 652 557 385 367 346 279 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2160 2451 2464 2472 513 Size: 552 1095 291 13 8 6892 Fragments arranged by size: 6892 1095 552 291 13 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2572 487 Size: 683 56 1346 6766 Fragments arranged by size: 6766 1346 683 56 BtsI GCAGTG_nn' Cuts at: 2308 2362 3169 3657 3738 6367 7161 7181 2308 Size: 54 807 488 81 2629 794 20 3978 Fragments arranged by size: 3978 2629 807 794 488 81 54 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3325 3360 529 Size: 35 2761 35 6020 Fragments arranged by size: 6020 2761 35 35 DraI TTT'AAA Cuts at: 4009 4477 6155 4009 Size: 468 1678 6705 Fragments arranged by size: 6705 1678 468 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4401 7858 809 Size: 507 3085 3457 1802 Fragments arranged by size: 3457 3085 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 2198 2202 Size: 54 1425 50 275 18 282 86 4 Cuts at: 2202 3654 6298 7187 8816 8 Size: 1452 2644 889 1629 43 Fragments arranged by size: 2644 1629 1452 1425 889 282 275 86 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2198 2202 6298 1537 Size: 275 386 4 4096 4090 Fragments arranged by size: 4096 4090 386 275 4 EarI CTCTTCn'nnn_ Cuts at: 1353 2415 2967 3784 6781 1353 Size: 1062 552 817 2997 3423 Fragments arranged by size: 3423 2997 1062 817 552 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2524 7438 7812 7958 387 Size: 1064 138 935 4914 374 146 1280 Fragments arranged by size: 4914 1280 1064 935 374 146 138 EcoICRI GAG'CTC Cuts at: 2238 3647 4901 5260 2238 Size: 1409 1254 359 5829 Fragments arranged by size: 5829 1409 1254 359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2696 2721 3053 5110 5589 5769 6914 Size: 393 1064 25 332 2057 479 180 1145 Cuts at: 6914 7511 8298 8566 1239 Size: 597 787 268 1524 Fragments arranged by size: 2057 1524 1145 1064 787 597 479 393 332 268 180 25 EcoNI CCTnn'n_nnAGG Cuts at: 5147 5147 Size: 8851 EcoO109I rG'GnC_Cy Cuts at: 3138 4315 4677 5308 3138 Size: 1177 362 631 6681 Fragments arranged by size: 6681 1177 631 362 EcoRI G'AATT_C Cuts at: 2744 4448 6160 6538 2744 Size: 1704 1712 378 5057 Fragments arranged by size: 5057 1712 1704 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2355 3288 4547 5329 6127 2355 Size: 933 1259 782 798 5079 Fragments arranged by size: 5079 1259 933 798 782 FseI GG_CCGG'CC Cuts at: 2204 2204 Size: 8851 FspI TGC'GCA Cuts at: 5943 7357 5943 Size: 1414 7437 HaeII r_GCGC'y Cuts at: 1678 2134 2154 2533 7998 1678 Size: 456 20 379 5465 2531 Fragments arranged by size: 5465 2531 456 379 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2414 2446 4627 4659 4890 Size: 32 135 32 842 32 2181 32 231 Cuts at: 4890 4922 4984 5016 7492 7524 7566 7598 1373 Size: 32 62 32 2476 32 42 32 2626 Fragments arranged by size: 2626 2476 2181 842 231 135 62 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6195 6441 669 Size: 988 281 4257 246 3079 Fragments arranged by size: 4257 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4787 5969 748 Size: 4039 1182 3630 Fragments arranged by size: 4039 3630 1182 HpaI GTT'AAC Cuts at: 6441 6441 Size: 8851 KpnI G_GTAC'C Cuts at: 4051 6176 4051 Size: 2125 6726 MfeI C'AATT_G Cuts at: 6450 6450 Size: 8851 MluI A'CGCG_T Cuts at: 8727 8727 Size: 8851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2332 3132 3700 4487 7965 8149 1305 Size: 1027 800 568 787 3478 184 2007 Fragments arranged by size: 3478 2007 1027 800 787 568 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3656 10 Size: 54 1425 343 1824 5205 Fragments arranged by size: 5205 1824 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3086 3264 3768 3936 4610 4721 5091 Size: 648 1920 178 504 168 674 111 370 Cuts at: 5091 5184 6809 7168 7327 518 Size: 93 1625 359 159 2042 Fragments arranged by size: 2042 1920 1625 674 648 504 370 359 178 168 159 111 93 MspA1I CmG'CkG Cuts at: 1606 2162 2207 2877 3471 6267 6944 8092 8337 Size: 556 45 670 594 2796 677 1148 245 Cuts at: 8337 1606 Size: 2120 Fragments arranged by size: 2796 2120 1148 677 670 594 556 245 45 NaeI GCC'GGC Cuts at: 2202 2202 Size: 8851 NcoI C'CATG_G Cuts at: 513 1065 2464 513 Size: 552 1399 6900 Fragments arranged by size: 6900 1399 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8851 NgoMIV G'CCGG_C Cuts at: 2200 2200 Size: 8851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8851 NotI GC'GGCC_GC Cuts at: 6298 6298 Size: 8851 NsiI A_TGCA'T Cuts at: 3210 4164 3210 Size: 954 7897 NspI r_CATG'y Cuts at: 1132 1229 3851 4162 4166 5695 6209 7754 1132 Size: 97 2622 311 4 1529 514 1545 2229 Fragments arranged by size: 2622 2229 1545 1529 514 311 97 4 PasI CC'CwG_GG Cuts at: 1348 2372 2623 3311 1348 Size: 1024 251 688 6888 Fragments arranged by size: 6888 1024 688 251 PciI A'CATG_T Cuts at: 3847 7750 3847 Size: 3903 4948 PflMI CCAn_nnn'nTGG Cuts at: 1108 2140 2318 3311 5029 5109 6133 1108 Size: 1032 178 993 1718 80 1024 3826 Fragments arranged by size: 3826 1718 1032 1024 993 178 80 PfoI T'CCnGG_A Cuts at: 3030 5208 3030 Size: 2178 6673 PmeI GTTT'AAAC Cuts at: 6155 6155 Size: 8851 PmlI CAC'GTG Cuts at: 5182 5182 Size: 8851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6920 6952 8433 8465 6920 Size: 32 1481 32 7306 Fragments arranged by size: 7306 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4315 4677 4315 Size: 362 8489 PshAI GACnn'nnGTC Cuts at: 1613 3502 4328 1613 Size: 1889 826 6136 Fragments arranged by size: 6136 1889 826 PsiI TTA'TAA Cuts at: 2385 6421 2385 Size: 4036 4815 PspOMI G'GGCC_C Cuts at: 3138 3138 Size: 8851 PstI C_TGCA'G Cuts at: 830 2546 4492 4555 6203 830 Size: 1716 1946 63 1648 3478 Fragments arranged by size: 3478 1946 1716 1648 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8851 PvuII CAG'CTG Cuts at: 2877 3471 2877 Size: 594 8257 SacI G_AGCT'C Cuts at: 2240 3649 4903 5262 2240 Size: 1409 1254 359 5829 Fragments arranged by size: 5829 1409 1254 359 SacII CC_GC'GG Cuts at: 2163 2163 Size: 8851 SalI G'TCGA_C Cuts at: 1936 6193 1936 Size: 4257 4594 SbfI CC_TGCA'GG Cuts at: 6203 6203 Size: 8851 ScaI AGT'ACT Cuts at: 5198 5741 7099 8811 5198 Size: 543 1358 1712 5238 Fragments arranged by size: 5238 1712 1358 543 SexAI A'CCwGG_T Cuts at: 2643 2643 Size: 8851 SfcI C'TryA_G Cuts at: 826 1045 2542 4223 4379 4488 4551 5781 6199 Size: 219 1497 1681 156 109 63 1230 418 Cuts at: 6199 7334 8015 8206 826 Size: 1135 681 191 1471 Fragments arranged by size: 1681 1497 1471 1230 1135 681 418 219 191 156 109 63 SfiI GGCCn_nnn'nGGCC Cuts at: 2201 2201 Size: 8851 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8851 SgrAI Cr'CCGG_yG Cuts at: 1700 5704 1700 Size: 4004 4847 SmaI CCC'GGG Cuts at: 1872 6178 1872 Size: 4306 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2824 4902 5303 6955 7856 8118 Size: 197 926 881 2078 401 1652 901 262 Cuts at: 8118 820 Size: 1553 Fragments arranged by size: 2078 1652 1553 926 901 881 401 262 197 SnaBI TAC'GTA Cuts at: 493 6642 493 Size: 6149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8851 SphI G_CATG'C Cuts at: 1132 6209 1132 Size: 5077 3774 SspI AAT'ATT Cuts at: 5 52 3343 5866 6775 5 Size: 47 3291 2523 909 2081 Fragments arranged by size: 3291 2523 2081 909 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8851 StyI C'CwwG_G Cuts at: 513 1065 2330 2464 3841 5999 6290 513 Size: 552 1265 134 1377 2158 291 3074 Fragments arranged by size: 3074 2158 1377 1265 552 291 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2573 3179 3623 4851 6878 6895 7048 Size: 1255 993 606 444 1228 2027 17 153 Cuts at: 7048 7233 7678 325 Size: 185 445 1498 Fragments arranged by size: 2027 1498 1255 1228 993 606 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2770 5196 5739 Size: 274 80 33 51 1443 793 2426 543 Cuts at: 5739 7097 8809 96 Size: 1358 1712 138 Fragments arranged by size: 2426 1712 1443 1358 793 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2619 2651 8811 8843 2619 Size: 32 6160 32 2627 Fragments arranged by size: 6160 2627 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4850 1316 Size: 3534 5317 XbaI T'CTAG_A Cuts at: 3506 3862 6187 3506 Size: 356 2325 6170 Fragments arranged by size: 6170 2325 356 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2315 2737 2315 Size: 422 8429 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8851 XmaI C'CCGG_G Cuts at: 1870 6176 1870 Size: 4306 4545 XmnI GAAnn'nnTTC Cuts at: 2833 5153 5574 6980 2833 Size: 2320 421 1406 4704 Fragments arranged by size: 4704 2320 1406 421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5233 6659 276 Size: 53 83 186 936 3699 1426 2468 Fragments arranged by size: 3699 2468 1426 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoRV FspAI KasI NarI NruI PacI PspXI PsrI RsrII SanDI SapI SfoI SrfI SwaI