(Linear) (Six-base) MAPSORT of: KIEE4279.seq Check: 461 from: 1 to: 4146 KIEE4279.orf With 182 enzymes: SgfI * March 22, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2224 4146 Size: 2224 1922 AatII G_ACGT'C Cuts at: 0 3230 4146 Size: 3230 916 Acc65I G'GTAC_C Cuts at: 0 2042 4146 Size: 2042 2104 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 691 3584 4146 Size: 691 2893 562 Fragments arranged by size: 2893 691 562 AfeI AGC'GCT Cuts at: 0 526 4146 Size: 526 3620 AflII C'TTAA_G Cuts at: 0 819 4146 Size: 819 3327 AflIII A'CryG_T Cuts at: 0 1842 4146 Size: 1842 2304 AgeI A'CCGG_T Cuts at: 0 1035 3699 4146 Size: 1035 2664 447 Fragments arranged by size: 2664 1035 447 AleI CACnn'nnGTG Cuts at: 0 2605 2716 3086 4146 Size: 2605 111 370 1060 Fragments arranged by size: 2605 1060 370 111 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3263 4146 Size: 3263 883 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2999 3031 4146 Size: 2999 32 1115 Fragments arranged by size: 2999 1115 32 AlwNI CAG_nnn'CTG Cuts at: 0 1306 1437 4146 Size: 1306 131 2709 Fragments arranged by size: 2709 1306 131 ApaI G_GGCC'C Cuts at: 0 1137 4146 Size: 1137 3009 ApaLI G'TGCA_C Cuts at: 0 3160 4146 Size: 3160 986 ApoI r'AATT_y Cuts at: 0 739 828 1899 1953 2443 2919 3028 3502 Size: 739 89 1071 54 490 476 109 474 Cuts at: 3502 4146 Size: 644 Fragments arranged by size: 1071 739 644 490 476 474 109 89 54 AscI GG'CGCG_CC Cuts at: 0 80 4146 Size: 80 4066 AvaI C'yCGr_G Cuts at: 0 3796 4146 Size: 3796 350 BanI G'GyrC_C Cuts at: 0 2042 2814 2856 4141 4146 Size: 2042 772 42 1285 5 Fragments arranged by size: 2042 1285 772 42 5 BanII G_rGCy'C Cuts at: 0 235 1137 1644 2898 3257 4146 Size: 235 902 507 1254 359 889 Fragments arranged by size: 1254 902 889 507 359 235 BbsI GAAGACnn'nnnn_ Cuts at: 0 2137 3141 3572 4146 Size: 2137 1004 431 574 Fragments arranged by size: 2137 1004 574 431 BbvCI CC'TCA_GC Cuts at: 0 2927 3682 4146 Size: 2927 755 464 Fragments arranged by size: 2927 755 464 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1626 1660 3675 3709 4146 Size: 1626 34 2015 34 437 Fragments arranged by size: 2015 1626 437 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2737 4146 Size: 2737 1409 BclI T'GATC_A Cuts at: 0 1557 3720 4146 Size: 1557 2163 426 Fragments arranged by size: 2163 1557 426 BfrBI ATG'CAT Cuts at: 0 1203 2157 4146 Size: 1203 954 1989 Fragments arranged by size: 1989 1203 954 BglI GCCn_nnn'nGGC Cuts at: 0 97 196 1920 4146 Size: 97 99 1724 2226 Fragments arranged by size: 2226 1724 99 97 BglII A'GATC_T Cuts at: 0 2688 2967 4146 Size: 2688 279 1179 Fragments arranged by size: 2688 1179 279 Bme1580I G_kGCm'C Cuts at: 0 1137 2819 3164 3350 4146 Size: 1137 1682 345 186 796 Fragments arranged by size: 1682 1137 796 345 186 BmgBI CAC'GTC Cuts at: 0 135 359 4146 Size: 135 224 3787 Fragments arranged by size: 3787 224 135 BmrI ACTGGGnnnn_n' Cuts at: 0 1131 3427 4146 Size: 1131 2296 719 Fragments arranged by size: 2296 1131 719 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3247 4146 Size: 3247 899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 716 1048 3105 3764 4146 Size: 716 332 2057 659 382 Fragments arranged by size: 2057 716 659 382 332 Bpu10I CC'TnA_GC Cuts at: 0 2593 2927 3682 4146 Size: 2593 334 755 464 Fragments arranged by size: 2593 755 464 334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2882 3283 4146 Size: 2882 401 863 Fragments arranged by size: 2882 863 401 BsaI GGTCTCn'nnnn_ Cuts at: 0 3042 4146 Size: 3042 1104 BsaAI yAC'GTr Cuts at: 0 3177 4146 Size: 3177 969 BsaBI GATnn'nnATC Cuts at: 0 2972 4146 Size: 2972 1174 BsaHI Gr'CG_yC Cuts at: 0 3227 3557 4146 Size: 3227 330 589 Fragments arranged by size: 3227 589 330 BsaWI w'CCGG_w Cuts at: 0 1035 3383 3699 4146 Size: 1035 2348 316 447 Fragments arranged by size: 2348 1035 447 316 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 614 644 2026 2056 3802 3832 4146 Size: 614 30 1382 30 1746 30 314 Fragments arranged by size: 1746 1382 614 314 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 130 1007 2293 3277 3670 4146 Size: 130 877 1286 984 393 476 Fragments arranged by size: 1286 984 877 476 393 130 BseYI C'CCAG_C Cuts at: 0 75 554 4146 Size: 75 479 3592 Fragments arranged by size: 3592 479 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 3114 3157 4146 Size: 142 2972 43 989 Fragments arranged by size: 2972 989 142 43 BsiEI CG_ry'CG Cuts at: 0 196 200 4080 4146 Size: 196 4 3880 66 Fragments arranged by size: 3880 196 66 4 BsiHKAI G_wGCw'C Cuts at: 0 235 995 1644 1722 2898 3164 3257 3803 Size: 235 760 649 78 1176 266 93 546 Cuts at: 3803 4139 4146 Size: 336 7 Fragments arranged by size: 1176 760 649 546 336 266 235 93 78 7 BsmI GAATG_Cn' Cuts at: 0 3587 4146 Size: 3587 559 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2589 3218 4146 Size: 2589 629 928 Fragments arranged by size: 2589 928 629 Bsp1286I G_dGCh'C Cuts at: 0 235 995 1137 1644 1722 2819 2898 3164 Size: 235 760 142 507 78 1097 79 266 Cuts at: 3164 3257 3350 3803 4139 4146 Size: 93 93 453 336 7 Fragments arranged by size: 1097 760 507 453 336 266 235 142 93 93 79 78 7 BspEI T'CCGG_A Cuts at: 0 3383 4146 Size: 3383 763 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1230 2224 4146 Size: 1230 994 1922 Fragments arranged by size: 1922 1230 994 BsrBI CCG'CTC Cuts at: 0 3125 4146 Size: 3125 1021 BsrDI GCAATG_nn' Cuts at: 0 1916 3950 4146 Size: 1916 2034 196 Fragments arranged by size: 2034 1916 196 BsrFI r'CCGG_y Cuts at: 0 195 1035 1517 3699 4146 Size: 195 840 482 2182 447 Fragments arranged by size: 2182 840 482 447 195 BssHII G'CGCG_C Cuts at: 0 80 4146 Size: 80 4066 BstAPI GCAn_nnn'nTGC Cuts at: 0 2863 4146 Size: 2863 1283 BstBI TT'CG_AA Cuts at: 0 209 4146 Size: 209 3937 BstEII G'GTnAC_C Cuts at: 0 57 549 4146 Size: 57 492 3597 Fragments arranged by size: 3597 492 57 BstXI CCAn_nnnn'nTGG Cuts at: 0 3417 4146 Size: 3417 729 BstYI r'GATC_y Cuts at: 0 2637 2688 2967 3619 4146 Size: 2637 51 279 652 527 Fragments arranged by size: 2637 652 527 279 51 BtgI C'CryG_G Cuts at: 0 155 446 459 467 4146 Size: 155 291 13 8 3679 Fragments arranged by size: 3679 291 155 13 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 567 4146 Size: 567 3579 BtsI GCAGTG_nn' Cuts at: 0 303 357 1164 1652 1733 4146 Size: 303 54 807 488 81 2413 Fragments arranged by size: 2413 807 488 303 81 54 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1320 1355 4146 Size: 1320 35 2791 Fragments arranged by size: 2791 1320 35 DraI TTT'AAA Cuts at: 0 2004 2472 4146 Size: 2004 468 1674 Fragments arranged by size: 2004 1674 468 DrdI GACnn_nn'nnGTC Cuts at: 0 2396 4146 Size: 2396 1750 EaeI y'GGCC_r Cuts at: 0 107 193 197 1649 4146 Size: 107 86 4 1452 2497 Fragments arranged by size: 2497 1452 107 86 4 EagI C'GGCC_G Cuts at: 0 193 197 4146 Size: 193 4 3949 Fragments arranged by size: 3949 193 4 EarI CTCTTCn'nnn_ Cuts at: 0 410 962 1779 4146 Size: 410 552 817 2367 Fragments arranged by size: 2367 817 552 410 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 519 4146 Size: 519 3627 EcoICRI GAG'CTC Cuts at: 0 233 1642 2896 3255 4146 Size: 233 1409 1254 359 891 Fragments arranged by size: 1409 1254 891 359 233 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 691 716 1048 3105 3584 3764 4146 Size: 691 25 332 2057 479 180 382 Fragments arranged by size: 2057 691 479 382 332 180 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 3142 4146 Size: 3142 1004 EcoO109I rG'GnC_Cy Cuts at: 0 1133 2310 2672 3303 4146 Size: 1133 1177 362 631 843 Fragments arranged by size: 1177 1133 843 631 362 EcoRI G'AATT_C Cuts at: 0 739 2443 4146 Size: 739 1704 1703 Fragments arranged by size: 1704 1703 739 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 350 1283 2542 3324 4122 4146 Size: 350 933 1259 782 798 24 Fragments arranged by size: 1259 933 798 782 350 24 FseI GG_CCGG'CC Cuts at: 0 199 4146 Size: 199 3947 FspI TGC'GCA Cuts at: 0 3938 4146 Size: 3938 208 HaeII r_GCGC'y Cuts at: 0 129 149 528 4146 Size: 129 20 379 3618 Fragments arranged by size: 3618 379 129 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 409 441 2622 2654 2885 2917 2979 3011 Size: 409 32 2181 32 231 32 62 32 Cuts at: 3011 4146 Size: 1135 Fragments arranged by size: 2181 1135 409 231 62 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 2782 3964 4146 Size: 2782 1182 182 Fragments arranged by size: 2782 1182 182 KpnI G_GTAC'C Cuts at: 0 2046 4146 Size: 2046 2100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 327 1127 1695 2482 4146 Size: 327 800 568 787 1664 Fragments arranged by size: 1664 800 787 568 327 MscI TGG'CCA Cuts at: 0 1651 4146 Size: 1651 2495 MslI CAynn'nnrTG Cuts at: 0 1081 1259 1763 1931 2605 2716 3086 3179 Size: 1081 178 504 168 674 111 370 93 Cuts at: 3179 4146 Size: 967 Fragments arranged by size: 1081 967 674 504 370 178 168 111 93 MspA1I CmG'CkG Cuts at: 0 157 202 872 1466 4146 Size: 157 45 670 594 2680 Fragments arranged by size: 2680 670 594 157 45 NaeI GCC'GGC Cuts at: 0 197 4146 Size: 197 3949 NcoI C'CATG_G Cuts at: 0 459 4146 Size: 459 3687 NgoMIV G'CCGG_C Cuts at: 0 195 4146 Size: 195 3951 NsiI A_TGCA'T Cuts at: 0 1205 2159 4146 Size: 1205 954 1987 Fragments arranged by size: 1987 1205 954 NspI r_CATG'y Cuts at: 0 1846 2157 2161 3690 4146 Size: 1846 311 4 1529 456 Fragments arranged by size: 1846 1529 456 311 4 PasI CC'CwG_GG Cuts at: 0 367 618 1306 4146 Size: 367 251 688 2840 Fragments arranged by size: 2840 688 367 251 PciI A'CATG_T Cuts at: 0 1842 4146 Size: 1842 2304 PflMI CCAn_nnn'nTGG Cuts at: 0 135 313 1306 3024 3104 4128 4146 Size: 135 178 993 1718 80 1024 18 Fragments arranged by size: 1718 1024 993 178 135 80 18 PfoI T'CCnGG_A Cuts at: 0 1025 3203 4146 Size: 1025 2178 943 Fragments arranged by size: 2178 1025 943 PmlI CAC'GTG Cuts at: 0 3177 4146 Size: 3177 969 PpuMI rG'GwC_Cy Cuts at: 0 2310 2672 4146 Size: 2310 362 1474 Fragments arranged by size: 2310 1474 362 PshAI GACnn'nnGTC Cuts at: 0 1497 2323 4146 Size: 1497 826 1823 Fragments arranged by size: 1823 1497 826 PsiI TTA'TAA Cuts at: 0 380 4146 Size: 380 3766 PspOMI G'GGCC_C Cuts at: 0 1133 4146 Size: 1133 3013 PstI C_TGCA'G Cuts at: 0 541 2487 2550 4146 Size: 541 1946 63 1596 Fragments arranged by size: 1946 1596 541 63 PvuII CAG'CTG Cuts at: 0 872 1466 4146 Size: 872 594 2680 Fragments arranged by size: 2680 872 594 SacI G_AGCT'C Cuts at: 0 235 1644 2898 3257 4146 Size: 235 1409 1254 359 889 Fragments arranged by size: 1409 1254 889 359 235 SacII CC_GC'GG Cuts at: 0 158 4146 Size: 158 3988 ScaI AGT'ACT Cuts at: 0 3193 3736 4146 Size: 3193 543 410 Fragments arranged by size: 3193 543 410 SexAI A'CCwGG_T Cuts at: 0 638 4146 Size: 638 3508 SfcI C'TryA_G Cuts at: 0 537 2218 2374 2483 2546 3776 4146 Size: 537 1681 156 109 63 1230 370 Fragments arranged by size: 1681 1230 537 370 156 109 63 SfiI GGCCn_nnn'nGGCC Cuts at: 0 196 4146 Size: 196 3950 SgrAI Cr'CCGG_yG Cuts at: 0 3699 4146 Size: 3699 447 SmlI C'TyrA_G Cuts at: 0 819 2897 3298 4146 Size: 819 2078 401 848 Fragments arranged by size: 2078 848 819 401 SspI AAT'ATT Cuts at: 0 1338 3861 4146 Size: 1338 2523 285 Fragments arranged by size: 2523 1338 285 StyI C'CwwG_G Cuts at: 0 325 459 1836 3994 4146 Size: 325 134 1377 2158 152 Fragments arranged by size: 2158 1377 325 152 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 568 1174 1618 2846 4146 Size: 568 606 444 1228 1300 Fragments arranged by size: 1300 1228 606 568 444 TatI w'GTAC_w Cuts at: 0 765 3191 3734 4146 Size: 765 2426 543 412 Fragments arranged by size: 2426 765 543 412 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 614 646 4146 Size: 614 32 3500 Fragments arranged by size: 3500 614 32 Tth111I GACn'n_nGTC Cuts at: 0 2845 4146 Size: 2845 1301 XbaI T'CTAG_A Cuts at: 0 1501 1857 4146 Size: 1501 356 2289 Fragments arranged by size: 2289 1501 356 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 310 732 4146 Size: 310 422 3414 Fragments arranged by size: 3414 422 310 XmnI GAAnn'nnTTC Cuts at: 0 828 3148 3569 4146 Size: 828 2320 421 577 Fragments arranged by size: 2320 828 577 421 ZraI GAC'GTC Cuts at: 0 3228 4146 Size: 3228 918 Enzymes that do cut: AarI AatII Acc65I AcuI AfeI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AvaI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PstI PvuII SacI SacII ScaI SexAI SfcI SfiI SgrAI SmlI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI ZraI Enzymes that do not cut: AccI AclI AhdI AseI AsiSI AvrII BaeI BamHI BbeI BlpI BmtI BsiWI BspHI BsrGI BssSI BstZ17I Bsu36I ClaI DraIII EcoRV FspAI HincII HpaI KasI MfeI MluI NarI NdeI NheI NotI NruI PacI PmeI PpiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI SfoI SgfI SmaI SnaBI SpeI SphI SrfI StuI SwaI XhoI XmaI