Restriction map for
pFN21AB8480
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII AccIII AclI AcyI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BcgI BcnI BfaI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiWI BsoBI Bsp1407I BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
CpoI DraI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FseI
FspI HaeII Hin1I KasI KspI
MaeI MamI MfeI MluI MroI
MunI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 173 GGTACC G^GTACC
AflII 2301 CTTAAG C^TTAAG
AocI 370 CCTGAGG CC^T[ACGT]AGG
ApaI 40 GGGCCC GGGCC^C
Asp700I 1891 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 173 GGTACC G^GTACC
BalI 759 TGGCCA TGG^CCA
BbvCI 857 CCTCAGC CCTCAGC(-5/-2)
BciVI 642 GGATAC GTATCC(6/5)
BfrI 2301 CTTAAG C^TTAAG
BglI 427 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 491 AGATCT A^GATCT
BpmI 2266 CTGGAG CTGGAG(16/14)
BsaWI 542 TCCGGT [AT]^CCGG[AT]
BsiEI 93 CGACCG CG[GA][CT]^CG
BsmI 268 GCATTC GAATGC(1/-1)
BspDI 1219 ATCGAT AT^CGAT
BstAPI 436 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 370 CCTGAGG CC^T[ACGT]AGG
Cac8I 732 GCAGGC GC[ACGT]^[ACGT]GC
ClaI 1219 ATCGAT AT^CGAT
DraII 40 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1397 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1983 GACTTTAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 759 TGGCCA [CT]^GGCC[GA]
Eco81I 370 CCTGAGG CC^T[ACGT]AGG
EcoNI 324 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 40 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 527 ATGCAT ATGCA^T
HapII 543 CCGG C^CGG
HgaI 283 GCGTC GACGC(5/10)
HincII 166 GTTAAC GT[CT]^[GA]AC
HindII 166 GTTAAC GT[CT]^[GA]AC
HpaI 166 GTTAAC GTT^AAC
HpaII 543 CCGG C^CGG
KpnI 173 GGTACC GGTAC^C
MluNI 759 TGGCCA TGG^CCA
MscI 759 TGGCCA TGG^CCA
MspA1I 1960 CAGCTG C[AC]G^C[GT]G
MspI 543 CCGG C^CGG
NdeI 2174 CATATG CA^TATG
NsiI 527 ATGCAT ATGCA^T
NspI 2415 ACATGC [GA]CATG^[CT]
PstI 337 CTGCAG CTGCA^G
PvuII 1960 CAGCTG CAG^CTG
SapI 1228 GAAGAGC GCTCTTC(1/4)
SexAI 1865 ACCAGGT A^CC[AT]GGT
StuI 2007 AGGCCT AGG^CCT
TfiI 2201 GAATC G^A[AT]TC
XmnI 1891 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 1531 GTAGAC GT^[AC][GT]AC
2557 GTAGAC
AccII 89 CGCG CG^CG
91 CGCG
AlwNI 57 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
2315 CAGAGGCTG
BamHI 102 GGATCC G^GATCC
220 GGATCC
BanI 159 GGCACC G^G[CT][GA]CC
173 GGTACC
BsaI 1855 GAGACC GGTCTC(1/5)
2296 GAGACC
BsmBI 261 CGTCTC CGTCTC(1/5)
418 GAGACG
BspHI 2087 TCATGA T^CATGA
2204 TCATGA
BstUI 89 CGCG CG^CG
91 CGCG
CfoI 90 GCGC GCG^C
2374 GCGC
EcoRI 1297 GAATTC G^AATTC
1372 GAATTC
EcoRV 745 GATATC GAT^ATC
1010 GATATC
HhaI 90 GCGC GCG^C
2374 GCGC
HinP1I 90 GCGC G^CGC
2374 GCGC
MvnI 89 CGCG CG^CG
91 CGCG
PflMI 393 CCAACCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1316 CCAATGACTGG
RcaI 2087 TCATGA T^CATGA
2204 TCATGA
SacI 505 GAGCTC GAGCT^C
1231 GAGCTC
ScaI 953 AGTACT AGT^ACT
1946 AGTACT
SfcI 337 CTGCAG C^T[GA][CT]AG
2430 CTACAG
Van91I 393 CCAACCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1316 CCAATGACTGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BclI BmyI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII