(Circular) (Six-base) MAPSORT of: pFN21AB8480.seq Check: 8019 from: 1 to: 7267 pFN21AB8480 7267 bp With 182 enzymes: SgfI * August 25, 2016 14:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5077 278 Size: 53 83 186 936 3541 2468 Fragments arranged by size: 3541 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3537 4563 4610 1937 Size: 1600 1026 47 4594 Fragments arranged by size: 4594 1600 1026 47 Acc65I G'GTAC_C Cuts at: 2178 4588 2178 Size: 2410 4857 AclI AA'CG_TT Cuts at: 1563 5394 5767 1563 Size: 3831 373 3063 Fragments arranged by size: 3831 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 2284 3096 3989 4381 4530 5330 6714 Size: 519 133 812 893 392 149 800 1384 Cuts at: 6714 6982 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 893 812 800 519 392 268 149 133 AflII C'TTAA_G Cuts at: 820 1017 4306 820 Size: 197 3289 3781 Fragments arranged by size: 3781 3289 197 AflIII A'CryG_T Cuts at: 1203 6166 7143 1203 Size: 4963 977 1327 Fragments arranged by size: 4963 1327 977 AgeI A'CCGG_T Cuts at: 6098 6098 Size: 7267 AhdI GACnn_n'nnGTC Cuts at: 5996 5996 Size: 7267 AleI CACnn'nnGTG Cuts at: 1166 2642 1166 Size: 1476 5791 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2760 1184 Size: 1576 5691 AlwNI CAG_nnn'CTG Cuts at: 2067 4325 5010 6582 2067 Size: 2258 685 1572 2752 Fragments arranged by size: 2752 2258 1572 685 ApaI G_GGCC'C Cuts at: 2049 2049 Size: 7267 ApaLI G'TGCA_C Cuts at: 5324 6480 5324 Size: 1156 6111 ApoI r'AATT_y Cuts at: 1493 2521 3302 3377 3591 3613 3677 3974 4576 Size: 1028 781 75 214 22 64 297 602 Cuts at: 4576 4802 4954 7063 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1028 781 602 297 226 214 152 75 64 22 AseI AT'TA_AT Cuts at: 160 1032 5821 160 Size: 872 4789 1606 Fragments arranged by size: 4789 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7267 AvaI C'yCGr_G Cuts at: 1870 1943 4592 1870 Size: 73 2649 4545 Fragments arranged by size: 4545 2649 73 BamHI G'GATC_C Cuts at: 2107 2225 4597 4964 2107 Size: 118 2372 367 4410 Fragments arranged by size: 4410 2372 367 118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2164 2178 4588 6043 618 Size: 325 217 627 377 14 2410 1455 1842 Fragments arranged by size: 2410 1842 1455 627 377 325 217 14 BanII G_rGCy'C Cuts at: 1391 2049 2514 3240 3388 3942 4586 1391 Size: 658 465 726 148 554 644 4072 Fragments arranged by size: 4072 726 658 644 554 465 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2952 3093 3135 3729 3893 3992 4380 Size: 971 1053 141 42 594 164 99 388 Cuts at: 4380 928 Size: 3815 Fragments arranged by size: 3815 1053 971 594 388 164 141 99 42 BbvCI CC'TCA_GC Cuts at: 2863 2863 Size: 7267 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5458 5492 1903 Size: 34 50 34 3437 34 3678 Fragments arranged by size: 3678 3437 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2641 5159 6369 2641 Size: 2518 1210 3539 Fragments arranged by size: 3539 2518 1210 BclI T'GATC_A Cuts at: 1548 2466 2679 2820 4443 1548 Size: 918 213 141 1623 4372 Fragments arranged by size: 4372 1623 918 213 141 BfrBI ATG'CAT Cuts at: 2534 2534 Size: 7267 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2438 5878 136 Size: 107 122 71 631 605 766 3440 1525 Fragments arranged by size: 3440 1525 766 631 605 122 107 71 BglII A'GATC_T Cuts at: 2496 7262 2496 Size: 4766 2501 BlpI GC'TnA_GC Cuts at: 4684 4684 Size: 7267 Bme1580I G_kGCm'C Cuts at: 1790 2049 2167 5328 6484 1790 Size: 259 118 3161 1156 2573 Fragments arranged by size: 3161 2573 1156 259 118 BmgBI CAC'GTC Cuts at: 1318 2266 3407 1318 Size: 948 1141 5178 Fragments arranged by size: 5178 1141 948 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2446 2565 3337 Size: 416 53 83 399 24 1029 119 772 Cuts at: 3337 4998 5956 442 Size: 1661 958 1753 Fragments arranged by size: 1753 1661 1029 958 772 416 399 119 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7267 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3709 3709 Size: 7267 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4292 5927 1239 Size: 3053 1635 2579 Fragments arranged by size: 3053 2579 1635 Bpu10I CC'TnA_GC Cuts at: 2360 2863 2360 Size: 503 6764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3785 4203 5392 6257 6555 3785 Size: 418 1189 865 298 4497 Fragments arranged by size: 4497 1189 865 418 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3854 4295 5930 882 Size: 617 2355 441 1635 2219 Fragments arranged by size: 2355 2219 1635 617 441 BsaAI yAC'GTr Cuts at: 493 1204 5058 493 Size: 711 3854 2702 Fragments arranged by size: 3854 2702 711 BsaBI GATnn'nnATC Cuts at: 7261 7261 Size: 7267 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5074 5456 6091 275 Size: 53 83 186 936 3541 382 635 1451 Fragments arranged by size: 3541 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2547 5700 6098 6372 6519 6930 2547 Size: 3153 398 274 147 411 2884 Fragments arranged by size: 3153 2884 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7042 Fragments arranged by size: 7042 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2128 2423 2426 2429 3305 3509 1186 Size: 942 295 3 3 876 204 4944 Fragments arranged by size: 4944 942 876 295 204 3 3 BseYI C'CCAG_C Cuts at: 6470 6966 6470 Size: 496 6771 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2337 2766 3796 1163 Size: 573 601 429 1030 4634 Fragments arranged by size: 4634 1030 601 573 429 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2101 4717 5478 6506 1540 Size: 275 186 100 2616 761 1028 2301 Fragments arranged by size: 2616 2301 1028 761 275 186 100 BsiHKAI G_wGCw'C Cuts at: 2514 3240 3412 5328 5413 6484 2514 Size: 726 172 1916 85 1071 3297 Fragments arranged by size: 3297 1916 1071 726 172 85 BsmI GAATG_Cn' Cuts at: 2273 4778 4871 2273 Size: 2505 93 4669 Fragments arranged by size: 4669 2505 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2272 2417 2272 Size: 145 7122 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2167 2514 3240 3388 3412 3942 Size: 399 259 118 347 726 148 24 530 Cuts at: 3942 4586 5328 5413 6484 1391 Size: 644 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 726 644 530 399 347 259 148 118 85 24 BspEI T'CCGG_A Cuts at: 6930 6930 Size: 7267 BspHI T'CATG_A Cuts at: 4092 4209 5156 6886 4092 Size: 117 947 1730 4473 Fragments arranged by size: 4473 1730 947 117 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4622 844 Size: 3778 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5154 1394 Size: 429 3331 3507 Fragments arranged by size: 3507 3331 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5762 5936 65 Size: 1183 4514 174 1396 Fragments arranged by size: 4514 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5911 6098 1700 Size: 110 4101 187 2869 Fragments arranged by size: 4101 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7267 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7267 BssSI C'ACGA_G Cuts at: 5327 6339 5327 Size: 1012 6255 BstAPI GCAn_nnn'nTGC Cuts at: 794 2447 794 Size: 1653 5614 BstBI TT'CG_AA Cuts at: 6071 6071 Size: 7267 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7267 BstYI r'GATC_y Cuts at: 1972 2107 2225 2338 2496 2566 3547 4351 4408 Size: 135 118 113 158 70 981 804 57 Cuts at: 4408 4431 4597 4964 5349 5366 6818 6904 6916 Size: 23 166 367 385 17 1452 86 12 Cuts at: 6916 7262 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 981 804 385 367 346 166 158 135 118 113 86 70 57 23 17 12 Bsu36I CC'TnA_GG Cuts at: 2376 2376 Size: 7267 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6715 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3078 487 Size: 683 56 1852 4676 Fragments arranged by size: 4676 1852 683 56 BtsI GCAGTG_nn' Cuts at: 2283 3351 4783 5577 5597 2283 Size: 1068 1432 794 20 3953 Fragments arranged by size: 3953 1432 1068 794 20 ClaI AT'CG_AT Cuts at: 1554 3225 1554 Size: 1671 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7232 DraI TTT'AAA Cuts at: 4571 4571 Size: 7267 DraIII CAC_nnn'GTG Cuts at: 3407 3407 Size: 7267 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3994 6274 809 Size: 507 2678 2280 1802 Fragments arranged by size: 2678 2280 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2764 4714 5603 Size: 54 1425 50 275 18 934 1950 889 Cuts at: 5603 7232 8 Size: 1629 43 Fragments arranged by size: 1950 1629 1425 934 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4714 1537 Size: 275 2902 4090 Fragments arranged by size: 4090 2902 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3108 3228 4032 4050 5197 1353 Size: 1755 120 804 18 1147 3423 Fragments arranged by size: 3423 1755 1147 804 120 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3668 5854 6228 6374 387 Size: 1064 138 2079 2186 374 146 1280 Fragments arranged by size: 2186 2079 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2512 3238 2512 Size: 726 6541 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2284 3096 3989 4292 4381 4530 Size: 393 519 133 812 893 303 89 149 Cuts at: 4530 5330 5927 6714 6982 1239 Size: 800 597 787 268 1524 Fragments arranged by size: 1524 893 812 800 787 597 519 393 303 268 149 133 89 EcoNI CCTnn'n_nnAGG Cuts at: 2333 2333 Size: 7267 EcoO109I rG'GnC_Cy Cuts at: 2046 2046 Size: 7267 EcoRI G'AATT_C Cuts at: 3302 3377 4576 4954 3302 Size: 75 1199 378 5615 Fragments arranged by size: 5615 1199 378 75 EcoRV GAT'ATC Cuts at: 2752 3017 2752 Size: 265 7002 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2692 2692 Size: 7267 FspI TGC'GCA Cuts at: 5773 5773 Size: 7267 HaeII r_GCGC'y Cuts at: 1678 6414 1678 Size: 4736 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3107 3139 3253 3285 3462 Size: 32 135 32 1535 32 114 32 177 Cuts at: 3462 3494 3781 3813 4529 4561 5908 5940 5982 Size: 32 287 32 716 32 1347 32 42 Cuts at: 5982 6014 1373 Size: 32 2626 Fragments arranged by size: 2626 1535 1347 716 287 177 135 114 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2173 4611 4857 669 Size: 988 281 235 2438 246 3079 Fragments arranged by size: 3079 2438 988 281 246 235 HindIII A'AGCT_T Cuts at: 748 2557 2730 3707 3743 748 Size: 1809 173 977 36 4272 Fragments arranged by size: 4272 1809 977 173 36 HpaI GTT'AAC Cuts at: 2173 4857 2173 Size: 2684 4583 KpnI G_GTAC'C Cuts at: 2182 4592 2182 Size: 2410 4857 MfeI C'AATT_G Cuts at: 4866 4866 Size: 7267 MluI A'CGCG_T Cuts at: 7143 7143 Size: 7267 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3105 3354 3985 6381 6565 1305 Size: 1800 249 631 2396 184 2007 Fragments arranged by size: 2396 2007 1800 631 249 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2766 10 Size: 54 1425 343 934 4511 Fragments arranged by size: 4511 1425 934 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2642 2835 3818 4156 5225 5584 5743 Size: 648 1476 193 983 338 1069 359 159 Cuts at: 5743 518 Size: 2042 Fragments arranged by size: 2042 1476 1069 983 648 359 338 193 159 MspA1I CmG'CkG Cuts at: 1606 3967 4683 5360 6508 6753 1606 Size: 2361 716 677 1148 245 2120 Fragments arranged by size: 2361 2120 1148 716 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6715 NdeI CA'TA_TG Cuts at: 387 4180 387 Size: 3793 3474 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7267 NotI GC'GGCC_GC Cuts at: 4714 4714 Size: 7267 NsiI A_TGCA'T Cuts at: 2536 2536 Size: 7267 NspI r_CATG'y Cuts at: 1132 1229 4424 4625 6170 1132 Size: 97 3195 201 1545 2229 Fragments arranged by size: 3195 2229 1545 201 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7267 PciI A'CATG_T Cuts at: 6166 6166 Size: 7267 PflMI CCAn_nnn'nTGG Cuts at: 1108 2404 3327 1108 Size: 1296 923 5048 Fragments arranged by size: 5048 1296 923 PmeI GTTT'AAAC Cuts at: 4571 4571 Size: 7267 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5336 5368 6849 6881 5336 Size: 32 1481 32 5722 Fragments arranged by size: 5722 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7267 PsiI TTA'TAA Cuts at: 4837 4837 Size: 7267 PspOMI G'GGCC_C Cuts at: 2045 2045 Size: 7267 PstI C_TGCA'G Cuts at: 830 2346 4619 830 Size: 1516 2273 3478 Fragments arranged by size: 3478 2273 1516 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7267 PvuII CAG'CTG Cuts at: 3967 3967 Size: 7267 SacI G_AGCT'C Cuts at: 2514 3240 2514 Size: 726 6541 SalI G'TCGA_C Cuts at: 1936 4609 1936 Size: 2673 4594 SapI GCTCTTCn'nnn_ Cuts at: 3228 3228 Size: 7267 SbfI CC_TGCA'GG Cuts at: 4619 4619 Size: 7267 ScaI AGT'ACT Cuts at: 2960 3953 5515 7227 2960 Size: 993 1562 1712 3000 Fragments arranged by size: 3000 1712 1562 993 SexAI A'CCwGG_T Cuts at: 3870 3870 Size: 7267 SfcI C'TryA_G Cuts at: 826 1045 2342 4435 4615 5750 6431 6622 826 Size: 219 1297 2093 180 1135 681 191 1471 Fragments arranged by size: 2093 1471 1297 1135 681 219 191 180 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7267 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7267 SmaI CCC'GGG Cuts at: 1872 4594 1872 Size: 2722 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3800 4218 4306 5371 6272 6534 Size: 197 926 1857 418 88 1065 901 262 Cuts at: 6534 820 Size: 1553 Fragments arranged by size: 1857 1553 1065 926 901 418 262 197 88 SnaBI TAC'GTA Cuts at: 493 5058 493 Size: 4565 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7267 SphI G_CATG'C Cuts at: 1132 4625 1132 Size: 3493 3774 SspI AAT'ATT Cuts at: 5 52 5191 5 Size: 47 5139 2081 Fragments arranged by size: 5139 2081 47 StuI AGG'CCT Cuts at: 1517 4014 1517 Size: 2497 4770 StyI C'CwwG_G Cuts at: 513 1065 2311 2667 2689 3864 4706 513 Size: 552 1246 356 22 1175 842 3074 Fragments arranged by size: 3074 1246 1175 842 552 356 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5294 5311 5464 5649 6094 325 Size: 1255 3714 17 153 185 445 1498 Fragments arranged by size: 3714 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2958 3219 3951 Size: 274 80 33 51 1443 981 261 732 Cuts at: 3951 5513 7225 96 Size: 1562 1712 138 Fragments arranged by size: 1712 1562 1443 981 732 274 261 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7227 7259 7227 Size: 32 7235 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7267 XbaI T'CTAG_A Cuts at: 4603 4603 Size: 7267 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2722 2774 4012 2722 Size: 52 1238 5977 Fragments arranged by size: 5977 1238 52 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7267 XmaI C'CCGG_G Cuts at: 1870 4592 1870 Size: 2722 4545 XmnI GAAnn'nnTTC Cuts at: 3900 5396 3900 Size: 1496 5771 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5075 276 Size: 53 83 186 936 3541 2468 Fragments arranged by size: 3541 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI