Restriction map for
pFN21AB8480
(Linear) (Six-base) MAPSORT of: KIBB8480.seq Check: 6490 from: 1 to: 2562
KIBB8480.orf
With 182 enzymes: SgfI *
August 25, 2016 10:58 ..
AccI GT'mk_AC
Cuts at: 0 1532 2558 2562
Size: 1532 1026 4
Fragments arranged by size:
1532 1026 4
Acc65I G'GTAC_C
Cuts at: 0 173 2562
Size: 173 2389
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 279 1091 1984 2376 2525 2562
Size: 146 133 812 893 392 149 37
Fragments arranged by size:
893 812 392 149 146 133 37
AflII C'TTAA_G
Cuts at: 0 2301 2562
Size: 2301 261
AleI CACnn'nnGTG
Cuts at: 0 637 2562
Size: 637 1925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 755 2562
Size: 755 1807
AlwNI CAG_nnn'CTG
Cuts at: 0 62 2320 2562
Size: 62 2258 242
Fragments arranged by size:
2258 242 62
ApaI G_GGCC'C
Cuts at: 0 44 2562
Size: 44 2518
ApoI r'AATT_y
Cuts at: 0 516 1297 1372 1586 1608 1672 1969 2562
Size: 516 781 75 214 22 64 297 593
Fragments arranged by size:
781 593 516 297 214 75 64 22
BamHI G'GATC_C
Cuts at: 0 102 220 2562
Size: 102 118 2342
Fragments arranged by size:
2342 118 102
BanI G'GyrC_C
Cuts at: 0 159 173 2562
Size: 159 14 2389
Fragments arranged by size:
2389 159 14
BanII G_rGCy'C
Cuts at: 0 44 509 1235 1383 1937 2562
Size: 44 465 726 148 554 625
Fragments arranged by size:
726 625 554 465 148 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 947 1088 1130 1724 1888 1987 2375 2562
Size: 947 141 42 594 164 99 388 187
Fragments arranged by size:
947 594 388 187 164 141 99 42
BbvCI CC'TCA_GC
Cuts at: 0 858 2562
Size: 858 1704
BciVI GTATCCnnnnn_n'
Cuts at: 0 636 2562
Size: 636 1926
BclI T'GATC_A
Cuts at: 0 461 674 815 2438 2562
Size: 461 213 141 1623 124
Fragments arranged by size:
1623 461 213 141 124
BfrBI ATG'CAT
Cuts at: 0 529 2562
Size: 529 2033
BglI GCCn_nnn'nGGC
Cuts at: 0 433 2562
Size: 433 2129
BglII A'GATC_T
Cuts at: 0 491 2562
Size: 491 2071
Bme1580I G_kGCm'C
Cuts at: 0 44 162 2562
Size: 44 118 2400
Fragments arranged by size:
2400 118 44
BmgBI CAC'GTC
Cuts at: 0 261 1402 2562
Size: 261 1141 1160
Fragments arranged by size:
1160 1141 261
BmrI ACTGGGnnnn_n'
Cuts at: 0 441 560 1332 2562
Size: 441 119 772 1230
Fragments arranged by size:
1230 772 441 119
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1704 2562
Size: 1704 858
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2287 2562
Size: 2287 275
Bpu10I CC'TnA_GC
Cuts at: 0 355 858 2562
Size: 355 503 1704
Fragments arranged by size:
1704 503 355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1780 2198 2562
Size: 1780 418 364
Fragments arranged by size:
1780 418 364
BsaI GGTCTCn'nnnn_
Cuts at: 0 1849 2290 2562
Size: 1849 441 272
Fragments arranged by size:
1849 441 272
BsaWI w'CCGG_w
Cuts at: 0 542 2562
Size: 542 2020
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 123 418 421 424 1300 1504 2562
Size: 123 295 3 3 876 204 1058
Fragments arranged by size:
1058 876 295 204 123 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 761 1791 2562
Size: 332 429 1030 771
Fragments arranged by size:
1030 771 429 332
BsiEI CG_ry'CG
Cuts at: 0 96 2562
Size: 96 2466
BsiHKAI G_wGCw'C
Cuts at: 0 509 1235 1407 2562
Size: 509 726 172 1155
Fragments arranged by size:
1155 726 509 172
BsmI GAATG_Cn'
Cuts at: 0 268 2562
Size: 268 2294
BsmBI CGTCTCn'nnnn_
Cuts at: 0 267 412 2562
Size: 267 145 2150
Fragments arranged by size:
2150 267 145
Bsp1286I G_dGCh'C
Cuts at: 0 44 162 509 1235 1383 1407 1937 2562
Size: 44 118 347 726 148 24 530 625
Fragments arranged by size:
726 625 530 347 148 118 44 24
BspHI T'CATG_A
Cuts at: 0 2087 2204 2562
Size: 2087 117 358
Fragments arranged by size:
2087 358 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 442 2562
Size: 442 2120
BstYI r'GATC_y
Cuts at: 0 102 220 333 491 561 1542 2346 2403
Size: 102 118 113 158 70 981 804 57
Cuts at: 2403 2426 2562
Size: 23 136
Fragments arranged by size:
981 804 158 136 118 113 102 70
57 23
Bsu36I CC'TnA_GG
Cuts at: 0 371 2562
Size: 371 2191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1073 2562
Size: 1073 1489
BtsI GCAGTG_nn'
Cuts at: 0 278 1346 2562
Size: 278 1068 1216
Fragments arranged by size:
1216 1068 278
ClaI AT'CG_AT
Cuts at: 0 1220 2562
Size: 1220 1342
DraIII CAC_nnn'GTG
Cuts at: 0 1402 2562
Size: 1402 1160
DrdI GACnn_nn'nnGTC
Cuts at: 0 1989 2562
Size: 1989 573
EaeI y'GGCC_r
Cuts at: 0 759 2562
Size: 759 1803
EarI CTCTTCn'nnn_
Cuts at: 0 1103 1223 2027 2045 2562
Size: 1103 120 804 18 517
Fragments arranged by size:
1103 804 517 120 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1663 2562
Size: 1663 899
EcoICRI GAG'CTC
Cuts at: 0 507 1233 2562
Size: 507 726 1329
Fragments arranged by size:
1329 726 507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 279 1091 1984 2287 2376 2525 2562
Size: 146 133 812 893 303 89 149 37
Fragments arranged by size:
893 812 303 149 146 133 89 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 2562
Size: 328 2234
EcoO109I rG'GnC_Cy
Cuts at: 0 41 2562
Size: 41 2521
EcoRI G'AATT_C
Cuts at: 0 1297 1372 2562
Size: 1297 75 1190
Fragments arranged by size:
1297 1190 75
EcoRV GAT'ATC
Cuts at: 0 747 1012 2562
Size: 747 265 1550
Fragments arranged by size:
1550 747 265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 687 2562
Size: 687 1875
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 1248 1280 1457 1489 1776 1808
Size: 1102 32 114 32 177 32 287 32
Cuts at: 1808 2524 2556 2562
Size: 716 32 6
Fragments arranged by size:
1102 716 287 177 114 32 32 32
32 32 6
HincII GTy'rAC
Cuts at: 0 168 2562
Size: 168 2394
HindIII A'AGCT_T
Cuts at: 0 552 725 1702 1738 2562
Size: 552 173 977 36 824
Fragments arranged by size:
977 824 552 173 36
HpaI GTT'AAC
Cuts at: 0 168 2562
Size: 168 2394
KpnI G_GTAC'C
Cuts at: 0 177 2562
Size: 177 2385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 1349 1980 2562
Size: 1100 249 631 582
Fragments arranged by size:
1100 631 582 249
MscI TGG'CCA
Cuts at: 0 761 2562
Size: 761 1801
MslI CAynn'nnrTG
Cuts at: 0 637 830 1813 2151 2562
Size: 637 193 983 338 411
Fragments arranged by size:
983 637 411 338 193
MspA1I CmG'CkG
Cuts at: 0 1962 2562
Size: 1962 600
NdeI CA'TA_TG
Cuts at: 0 2175 2562
Size: 2175 387
NsiI A_TGCA'T
Cuts at: 0 531 2562
Size: 531 2031
NspI r_CATG'y
Cuts at: 0 2419 2562
Size: 2419 143
PflMI CCAn_nnn'nTGG
Cuts at: 0 399 1322 2562
Size: 399 923 1240
Fragments arranged by size:
1240 923 399
PspOMI G'GGCC_C
Cuts at: 0 40 2562
Size: 40 2522
PstI C_TGCA'G
Cuts at: 0 341 2562
Size: 341 2221
PvuII CAG'CTG
Cuts at: 0 1962 2562
Size: 1962 600
SacI G_AGCT'C
Cuts at: 0 509 1235 2562
Size: 509 726 1327
Fragments arranged by size:
1327 726 509
SapI GCTCTTCn'nnn_
Cuts at: 0 1223 2562
Size: 1223 1339
ScaI AGT'ACT
Cuts at: 0 955 1948 2562
Size: 955 993 614
Fragments arranged by size:
993 955 614
SexAI A'CCwGG_T
Cuts at: 0 1865 2562
Size: 1865 697
SfcI C'TryA_G
Cuts at: 0 337 2430 2562
Size: 337 2093 132
Fragments arranged by size:
2093 337 132
SmlI C'TyrA_G
Cuts at: 0 1795 2213 2301 2562
Size: 1795 418 88 261
Fragments arranged by size:
1795 418 261 88
StuI AGG'CCT
Cuts at: 0 2009 2562
Size: 2009 553
StyI C'CwwG_G
Cuts at: 0 306 662 684 1859 2562
Size: 306 356 22 1175 703
Fragments arranged by size:
1175 703 356 306 22
TatI w'GTAC_w
Cuts at: 0 953 1214 1946 2562
Size: 953 261 732 616
Fragments arranged by size:
953 732 616 261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 769 2007 2562
Size: 717 52 1238 555
Fragments arranged by size:
1238 717 555 52
XmnI GAAnn'nnTTC
Cuts at: 0 1895 2562
Size: 1895 667
Enzymes that do cut:
AccI Acc65I AcuI AflII AleI AlfI AlwNI ApaI
ApoI BamHI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaWI BseRI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BstAPI BstYI Bsu36I BtgZI
BtsI ClaI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII HpaI KpnI MmeI MscI MslI MspA1I NdeI
NsiI NspI PflMI PspOMI PstI PvuII SacI SapI
ScaI SexAI SfcI SmlI StuI StyI TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BcgI BlpI BmtI BsaAI BsaBI BsaHI BsaXI BseYI
BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I BtgI CspCI DraI
EagI FseI FspI FspAI HaeII KasI MfeI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI ZraI