################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BfaI BfrI BglII BlnI BpmI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstBI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcyI 547 GGCGTC G[GA]^CG[CT]C Alw44I 814 GTGCAC G^TGCAC ApaI 385 GGGCCC GGGCC^C ApaLI 814 GTGCAC G^TGCAC AspI 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 509 TGGCCA TGG^CCA BbsI 973 GTCTTC GAAGAC(2/6) BcgI 554 GCACTGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 395 TGATCA T^GATCA BlpI 747 GCTGAGC GC^T[ACGT]AGC Bpu1102I 747 GCTGAGC GC^T[ACGT]AGC BpuAI 973 GTCTTC GAAGAC(2/6) BsaHI 547 GGCGTC G[GA]^CG[CT]C BsiEI 8 CGACCG CG[GA][CT]^CG BsrI 165 CCAGT ACTGG(1/-1) BssSI 817 CACGAG CACGAG(-5/-1) BstXI 941 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 747 GCTGAGC GC^T[ACGT]AGC EcoNI 894 CCTCACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 1064 GCGGG CCCGC(4/6) FbaI 395 TGATCA T^GATCA Hin1I 547 GGCGTC G[GA]^CG[CT]C HincII 1081 GTCAAC GT[CT]^[GA]AC HindII 1081 GTCAAC GT[CT]^[GA]AC MluNI 509 TGGCCA TGG^CCA MscI 509 TGGCCA TGG^CCA NspI 1022 ACATGC [GA]CATG^[CT] PflFI 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 197 GACTC GAGTC(4/5) PvuII 516 CAGCTG CAG^CTG SfaNI 848 GCATC GCATC(5/9) TfiI 104 GATTC G^A[AT]TC Tsp509I 493 AATT ^AATT Tth111I 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 54 CCACAGCACCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 354 GTAC GT^AC 1056 GTAC BsaI 991 GAGACC GGTCTC(1/5) 721 GGTCTC BsmAI 991 GAGAC GTCTC(1/5) 722 GTCTC BsmI 73 GAATGC GAATGC(1/-1) 334 GAATGC BssHII 741 GCGCGC G^CGCGC 743 GCGCGC BstEII 624 GGTGACC G^GT[ACGT]ACC 762 GGTCACC BstPI 624 GGTGACC G^GT[ACGT]ACC 762 GGTCACC DdeI 486 CTCAG C^T[ACGT]AG 748 CTGAG DrdI 993 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1072 GACCTGGCCGTC EcoO65I 624 GGTGACC G^GT[ACGT]ACC 762 GGTCACC HgaI 26 GCGTC GACGC(5/10) 548 GCGTC HinfI 104 GATTC G^A[ACGT]TC 197 GACTC MslI 426 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 678 CACCCAGGTG NaeI 592 GCCGGC GCC^GGC 1132 GCCGGC NgoMIV 592 GCCGGC G^CCGGC 1132 GCCGGC PstI 612 CTGCAG CTGCA^G 670 CTGCAG RsaI 354 GTAC GT^AC 1056 GTAC SacI 247 GAGCTC GAGCT^C 871 GAGCTC SfcI 612 CTGCAG C^T[GA][CT]AG 670 CTGCAG SmaI 211 CCCGGG CCC^GGG 349 CCCGGG TaqI 602 TCGA T^CGA 1106 TCGA TthHB8I 602 TCGA T^CGA 1106 TCGA XmaCI 211 CCCGGG C^CCGGG 349 CCCGGG XmaI 211 CCCGGG C^CCGGG 349 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AluI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI TseI Tsp45I TspRI