(Circular) (Six-base) MAPSORT of: pFN21AE4365.seq Check: 5532 from: 1 to: 5854 pFN21AE4365 5854 bp With 182 enzymes: SgfI * March 28, 2012 13:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2124 2441 2678 2124 Size: 317 237 5300 Fragments arranged by size: 5300 317 237 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3664 278 Size: 53 83 186 936 2128 2468 Fragments arranged by size: 2468 2128 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3197 1937 Size: 1260 4594 Acc65I G'GTAC_C Cuts at: 3175 3175 Size: 5854 AclI AA'CG_TT Cuts at: 1563 3981 4354 1563 Size: 2418 373 3063 Fragments arranged by size: 3063 2418 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2879 3041 3109 3917 5301 5569 1632 Size: 1247 162 68 808 1384 268 1917 Fragments arranged by size: 1917 1384 1247 808 268 162 68 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5657 AflIII A'CryG_T Cuts at: 1203 4753 5730 1203 Size: 3550 977 1327 Fragments arranged by size: 3550 1327 977 AgeI A'CCGG_T Cuts at: 4685 4685 Size: 5854 AhdI GACnn_n'nnGTC Cuts at: 4583 4583 Size: 5854 AleI CACnn'nnGTG Cuts at: 1166 2435 2687 1166 Size: 1269 252 4333 Fragments arranged by size: 4333 1269 252 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2690 1184 Size: 1506 4348 AlwNI CAG_nnn'CTG Cuts at: 3597 5169 3597 Size: 1572 4282 ApaI G_GGCC'C Cuts at: 2394 2394 Size: 5854 ApaLI G'TGCA_C Cuts at: 2819 3911 5067 2819 Size: 1092 1156 3606 Fragments arranged by size: 3606 1156 1092 ApoI r'AATT_y Cuts at: 1493 3163 3389 3541 5650 1493 Size: 1670 226 152 2109 1697 Fragments arranged by size: 2109 1697 1670 226 152 AseI AT'TA_AT Cuts at: 160 1032 4408 160 Size: 872 3376 1606 Fragments arranged by size: 3376 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5854 AvaI C'yCGr_G Cuts at: 1870 1943 2216 2354 2362 2714 3101 3179 1870 Size: 73 273 138 8 352 387 78 4545 Fragments arranged by size: 4545 387 352 273 138 78 73 8 BamHI G'GATC_C Cuts at: 3184 3551 3184 Size: 367 5487 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2136 2429 2589 2666 3175 Size: 325 217 627 349 293 160 77 509 Cuts at: 3175 4630 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 509 349 325 293 217 160 77 BanII G_rGCy'C Cuts at: 1391 2256 2394 2759 2880 3173 1391 Size: 865 138 365 121 293 4072 Fragments arranged by size: 4072 865 365 293 138 121 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2971 928 Size: 971 1072 3811 Fragments arranged by size: 3811 1072 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2548 2582 4045 4079 1903 Size: 34 611 34 1463 34 3678 Fragments arranged by size: 3678 1463 611 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3746 4956 3746 Size: 1210 4644 BclI T'GATC_A Cuts at: 1548 2400 1548 Size: 852 5002 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2145 2149 2484 Size: 107 122 71 631 605 473 4 335 Cuts at: 2484 2598 4465 136 Size: 114 1867 1525 Fragments arranged by size: 1867 1525 631 605 473 335 122 114 107 71 4 BglII A'GATC_T Cuts at: 5849 5849 Size: 5854 BlpI GC'TnA_GC Cuts at: 2753 3271 2753 Size: 518 5336 Bme1580I G_kGCm'C Cuts at: 1790 2353 2394 2480 2594 2669 2823 2966 3915 Size: 563 41 86 114 75 154 143 949 Cuts at: 3915 5071 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 949 563 154 143 114 86 75 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5854 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3585 4543 442 Size: 416 53 83 399 24 2168 958 1753 Fragments arranged by size: 2168 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4514 1239 Size: 3275 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3979 4844 5142 3979 Size: 865 298 4691 Fragments arranged by size: 4691 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2732 2990 4517 882 Size: 617 1233 258 1527 2219 Fragments arranged by size: 2219 1527 1233 617 258 BsaAI yAC'GTr Cuts at: 493 1204 3645 493 Size: 711 2441 2702 Fragments arranged by size: 2702 2441 711 BsaBI GATnn'nnATC Cuts at: 5848 5848 Size: 5854 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2553 3661 4043 4678 Size: 53 83 186 936 1020 1108 382 635 Cuts at: 4678 275 Size: 1451 Fragments arranged by size: 1451 1108 1020 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4287 4685 4959 5106 5517 4287 Size: 398 274 147 411 4624 Fragments arranged by size: 4624 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5629 Fragments arranged by size: 5629 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2272 2888 3161 1186 Size: 1086 616 273 3879 Fragments arranged by size: 3879 1086 616 273 BseYI C'CCAG_C Cuts at: 2241 2593 2760 2959 5057 5553 2241 Size: 352 167 199 2098 496 2542 Fragments arranged by size: 2542 2098 496 352 199 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2509 2542 2855 1163 Size: 573 773 33 313 4162 Fragments arranged by size: 4162 773 573 313 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 3304 4065 5093 1540 Size: 275 186 15 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2256 2823 2850 2880 2943 3915 4000 5071 2256 Size: 567 27 30 63 972 85 1071 3039 Fragments arranged by size: 3039 1071 972 567 85 63 30 27 BsmI GAATG_Cn' Cuts at: 2084 2345 3365 3458 2084 Size: 261 1020 93 4480 Fragments arranged by size: 4480 1020 261 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2256 2353 2394 2480 2594 2669 2759 Size: 399 466 97 41 86 114 75 90 Cuts at: 2759 2823 2850 2880 2943 2966 3173 3915 4000 Size: 64 27 30 63 23 207 742 85 Cuts at: 4000 5071 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 466 399 207 114 97 90 86 85 75 64 63 41 30 27 23 BspEI T'CCGG_A Cuts at: 5517 5517 Size: 5854 BspHI T'CATG_A Cuts at: 3743 5473 3743 Size: 1730 4124 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 2318 2441 2624 2678 2755 3021 3057 Size: 1280 194 123 183 54 77 266 36 Cuts at: 3057 3209 844 Size: 152 3489 Fragments arranged by size: 3489 1280 266 194 183 152 123 77 54 36 BsrBI CCG'CTC Cuts at: 1394 1823 3741 1394 Size: 429 1918 3507 Fragments arranged by size: 3507 1918 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4349 4523 65 Size: 1183 3101 174 1396 Fragments arranged by size: 3101 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2586 2597 2830 3137 4498 4685 1700 Size: 110 776 11 233 307 1361 187 2869 Fragments arranged by size: 2869 1361 776 307 233 187 110 11 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5854 BssHII G'CGCG_C Cuts at: 1925 2746 2748 1925 Size: 821 2 5031 Fragments arranged by size: 5031 821 2 BssSI C'ACGA_G Cuts at: 2822 3914 4926 2822 Size: 1092 1012 3750 Fragments arranged by size: 3750 1092 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2502 2652 2827 794 Size: 1708 150 175 3821 Fragments arranged by size: 3821 1708 175 150 BstBI TT'CG_AA Cuts at: 4658 4658 Size: 5854 BstEII G'GTnAC_C Cuts at: 1184 2629 2767 1184 Size: 1445 138 4271 Fragments arranged by size: 4271 1445 138 BstXI CCAn_nnnn'nTGG Cuts at: 2953 2953 Size: 5854 BstYI r'GATC_y Cuts at: 1972 3184 3551 3936 3953 5405 5491 5503 5849 Size: 1212 367 385 17 1452 86 12 346 Cuts at: 5849 1972 Size: 1977 Fragments arranged by size: 1977 1452 1212 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5302 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5115 Fragments arranged by size: 5115 683 56 BtsI GCAGTG_nn' Cuts at: 2228 3370 4164 4184 2228 Size: 1142 794 20 3898 Fragments arranged by size: 3898 1142 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5854 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5819 DraI TTT'AAA Cuts at: 3158 3158 Size: 5854 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3004 3083 4861 809 Size: 507 1688 79 1778 1802 Fragments arranged by size: 1802 1778 1688 507 79 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2056 2514 3081 Size: 54 1425 50 275 18 226 458 567 Cuts at: 3081 3301 4190 5819 8 Size: 220 889 1629 43 Fragments arranged by size: 1629 1425 889 567 458 275 226 220 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3301 1537 Size: 275 1489 4090 Fragments arranged by size: 4090 1489 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3784 1353 Size: 2431 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4441 4815 4961 387 Size: 1064 138 2852 374 146 1280 Fragments arranged by size: 2852 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2254 2878 2254 Size: 624 5230 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2879 3041 3109 3917 4514 5301 5569 Size: 393 1247 162 68 808 597 787 268 Cuts at: 5569 1239 Size: 1524 Fragments arranged by size: 1524 1247 808 787 597 393 268 162 68 EcoNI CCTnn'n_nnAGG Cuts at: 2903 2903 Size: 5854 EcoO109I rG'GnC_Cy Cuts at: 2156 2307 2391 3076 2156 Size: 151 84 685 4934 Fragments arranged by size: 4934 685 151 84 EcoRI G'AATT_C Cuts at: 3163 3541 3163 Size: 378 5476 FspI TGC'GCA Cuts at: 4360 4360 Size: 5854 HaeII r_GCGC'y Cuts at: 1678 5001 1678 Size: 3323 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4495 4527 4569 4601 1373 Size: 32 135 32 2923 32 42 32 2626 Fragments arranged by size: 2923 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3088 3198 3444 669 Size: 988 281 1150 110 246 3079 Fragments arranged by size: 3079 1150 988 281 246 110 HindIII A'AGCT_T Cuts at: 748 748 Size: 5854 HpaI GTT'AAC Cuts at: 3444 3444 Size: 5854 KpnI G_GTAC'C Cuts at: 3179 3179 Size: 5854 MfeI C'AATT_G Cuts at: 3453 3453 Size: 5854 MluI A'CGCG_T Cuts at: 5730 5730 Size: 5854 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3033 4968 5152 1305 Size: 1728 1935 184 2007 Fragments arranged by size: 2007 1935 1728 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2516 10 Size: 54 1425 343 684 3348 Fragments arranged by size: 3348 1425 684 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2435 2687 3812 4171 4330 518 Size: 648 1269 252 1125 359 159 2042 Fragments arranged by size: 2042 1269 1125 648 359 252 159 MspA1I CmG'CkG Cuts at: 1606 2523 2583 3069 3270 3947 5095 5340 1606 Size: 917 60 486 201 677 1148 245 2120 Fragments arranged by size: 2120 1148 917 677 486 245 201 60 NaeI GCC'GGC Cuts at: 2599 3139 2599 Size: 540 5314 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5302 NdeI CA'TA_TG Cuts at: 387 387 Size: 5854 NgoMIV G'CCGG_C Cuts at: 2597 3137 2597 Size: 540 5314 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5854 NotI GC'GGCC_GC Cuts at: 3301 3301 Size: 5854 NspI r_CATG'y Cuts at: 1132 1229 3031 3212 4757 1132 Size: 97 1802 181 1545 2229 Fragments arranged by size: 2229 1802 1545 181 97 PasI CC'CwG_GG Cuts at: 1348 2813 1348 Size: 1465 4389 PciI A'CATG_T Cuts at: 4753 4753 Size: 5854 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5854 PmeI GTTT'AAAC Cuts at: 3158 3158 Size: 5854 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3923 3955 5436 5468 3923 Size: 32 1481 32 4309 Fragments arranged by size: 4309 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2156 2307 3076 2156 Size: 151 769 4934 Fragments arranged by size: 4934 769 151 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5854 PsiI TTA'TAA Cuts at: 3424 3424 Size: 5854 PspOMI G'GGCC_C Cuts at: 2390 2390 Size: 5854 PstI C_TGCA'G Cuts at: 830 2621 2679 3206 830 Size: 1791 58 527 3478 Fragments arranged by size: 3478 1791 527 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5854 PvuII CAG'CTG Cuts at: 2523 2523 Size: 5854 SacI G_AGCT'C Cuts at: 2256 2880 2256 Size: 624 5230 SalI G'TCGA_C Cuts at: 1936 3196 1936 Size: 1260 4594 SanDI GG'GwC_CC Cuts at: 2156 2156 Size: 5854 SbfI CC_TGCA'GG Cuts at: 3206 3206 Size: 5854 ScaI AGT'ACT Cuts at: 4102 5814 4102 Size: 1712 4142 SfcI C'TryA_G Cuts at: 826 1045 2617 2675 3202 4337 5018 5209 826 Size: 219 1572 58 527 1135 681 191 1471 Fragments arranged by size: 1572 1471 1135 681 527 219 191 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5854 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5854 SmaI CCC'GGG Cuts at: 1872 2218 2356 3181 1872 Size: 346 138 825 4545 Fragments arranged by size: 4545 825 346 138 SmlI C'TyrA_G Cuts at: 820 1017 1943 3958 4859 5121 820 Size: 197 926 2015 901 262 1553 Fragments arranged by size: 2015 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3645 493 Size: 3152 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5854 SphI G_CATG'C Cuts at: 1132 3212 1132 Size: 2080 3774 SspI AAT'ATT Cuts at: 5 52 3778 5 Size: 47 3726 2081 Fragments arranged by size: 3726 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5854 StyI C'CwwG_G Cuts at: 513 1065 3293 513 Size: 552 2228 3074 Fragments arranged by size: 3074 2228 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 2699 2992 3881 3898 4051 4236 Size: 1255 450 669 293 889 17 153 185 Cuts at: 4236 4681 325 Size: 445 1498 Fragments arranged by size: 1498 1255 889 669 450 445 293 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3060 4100 5812 Size: 274 80 33 51 1443 1083 1040 1712 Cuts at: 5812 96 Size: 138 Fragments arranged by size: 1712 1443 1083 1040 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5814 5846 5814 Size: 32 5822 Tth111I GACn'n_nGTC Cuts at: 1316 2552 1316 Size: 1236 4618 XbaI T'CTAG_A Cuts at: 3190 3190 Size: 5854 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2066 2066 Size: 5854 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5854 XmaI C'CCGG_G Cuts at: 1870 2216 2354 3179 1870 Size: 346 138 825 4545 Fragments arranged by size: 4545 825 346 138 XmnI GAAnn'nnTTC Cuts at: 3983 3983 Size: 5854 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3662 276 Size: 53 83 186 936 2128 2468 Fragments arranged by size: 2468 2128 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI