(Linear) (Six-base) MAPSORT of: KIEE4365.seq Check: 6822 from: 1 to: 1149 KIEE4365.orf With 182 enzymes: SgfI * March 22, 2012 17:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 119 436 673 1149 Size: 119 317 237 476 Fragments arranged by size: 476 317 237 119 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 874 1036 1104 1149 Size: 874 162 68 45 Fragments arranged by size: 874 162 68 45 AleI CACnn'nnGTG Cuts at: 0 430 682 1149 Size: 430 252 467 Fragments arranged by size: 467 430 252 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 685 1149 Size: 685 464 ApaI G_GGCC'C Cuts at: 0 389 1149 Size: 389 760 ApaLI G'TGCA_C Cuts at: 0 814 1149 Size: 814 335 AvaI C'yCGr_G Cuts at: 0 211 349 357 709 1096 1149 Size: 211 138 8 352 387 53 Fragments arranged by size: 387 352 211 138 53 8 BanI G'GyrC_C Cuts at: 0 131 424 584 661 1149 Size: 131 293 160 77 488 Fragments arranged by size: 488 293 160 131 77 BanII G_rGCy'C Cuts at: 0 251 389 754 875 1149 Size: 251 138 365 121 274 Fragments arranged by size: 365 274 251 138 121 BbsI GAAGACnn'nnnn_ Cuts at: 0 966 1149 Size: 966 183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 543 577 1149 Size: 543 34 572 Fragments arranged by size: 572 543 34 BclI T'GATC_A Cuts at: 0 395 1149 Size: 395 754 BglI GCCn_nnn'nGGC Cuts at: 0 140 144 479 593 1149 Size: 140 4 335 114 556 Fragments arranged by size: 556 335 140 114 4 BlpI GC'TnA_GC Cuts at: 0 748 1149 Size: 748 401 Bme1580I G_kGCm'C Cuts at: 0 348 389 475 589 664 818 961 1149 Size: 348 41 86 114 75 154 143 188 Fragments arranged by size: 348 188 154 143 114 86 75 41 BsaI GGTCTCn'nnnn_ Cuts at: 0 727 985 1149 Size: 727 258 164 Fragments arranged by size: 727 258 164 BsaHI Gr'CG_yC Cuts at: 0 548 1149 Size: 548 601 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 267 883 1149 Size: 267 616 266 Fragments arranged by size: 616 267 266 BseYI C'CCAG_C Cuts at: 0 236 588 755 954 1149 Size: 236 352 167 199 195 Fragments arranged by size: 352 236 199 195 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 504 537 850 1149 Size: 504 33 313 299 Fragments arranged by size: 504 313 299 33 BsiEI CG_ry'CG Cuts at: 0 11 1149 Size: 11 1138 BsiHKAI G_wGCw'C Cuts at: 0 251 818 845 875 938 1149 Size: 251 567 27 30 63 211 Fragments arranged by size: 567 251 211 63 30 27 BsmI GAATG_Cn' Cuts at: 0 79 340 1149 Size: 79 261 809 Fragments arranged by size: 809 261 79 Bsp1286I G_dGCh'C Cuts at: 0 251 348 389 475 589 664 754 818 Size: 251 97 41 86 114 75 90 64 Cuts at: 818 845 875 938 961 1149 Size: 27 30 63 23 188 Fragments arranged by size: 251 188 114 97 90 86 75 64 63 41 30 27 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 119 313 436 619 673 750 1016 1052 Size: 119 194 123 183 54 77 266 36 Cuts at: 1052 1149 Size: 97 Fragments arranged by size: 266 194 183 123 119 97 77 54 36 BsrFI r'CCGG_y Cuts at: 0 581 592 825 1132 1149 Size: 581 11 233 307 17 Fragments arranged by size: 581 307 233 17 11 BssHII G'CGCG_C Cuts at: 0 741 743 1149 Size: 741 2 406 Fragments arranged by size: 741 406 2 BssSI C'ACGA_G Cuts at: 0 817 1149 Size: 817 332 BstAPI GCAn_nnn'nTGC Cuts at: 0 497 647 822 1149 Size: 497 150 175 327 Fragments arranged by size: 497 327 175 150 BstEII G'GTnAC_C Cuts at: 0 624 762 1149 Size: 624 138 387 Fragments arranged by size: 624 387 138 BstXI CCAn_nnnn'nTGG Cuts at: 0 948 1149 Size: 948 201 BtsI GCAGTG_nn' Cuts at: 0 223 1149 Size: 223 926 DrdI GACnn_nn'nnGTC Cuts at: 0 999 1078 1149 Size: 999 79 71 Fragments arranged by size: 999 79 71 EaeI y'GGCC_r Cuts at: 0 51 509 1076 1149 Size: 51 458 567 73 Fragments arranged by size: 567 458 73 51 EcoICRI GAG'CTC Cuts at: 0 249 873 1149 Size: 249 624 276 Fragments arranged by size: 624 276 249 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 874 1036 1104 1149 Size: 874 162 68 45 Fragments arranged by size: 874 162 68 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 898 1149 Size: 898 251 EcoO109I rG'GnC_Cy Cuts at: 0 151 302 386 1071 1149 Size: 151 151 84 685 78 Fragments arranged by size: 685 151 151 84 78 HincII GTy'rAC Cuts at: 0 1083 1149 Size: 1083 66 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1028 1149 Size: 1028 121 MscI TGG'CCA Cuts at: 0 511 1149 Size: 511 638 MslI CAynn'nnrTG Cuts at: 0 430 682 1149 Size: 430 252 467 Fragments arranged by size: 467 430 252 MspA1I CmG'CkG Cuts at: 0 518 578 1064 1149 Size: 518 60 486 85 Fragments arranged by size: 518 486 85 60 NaeI GCC'GGC Cuts at: 0 594 1134 1149 Size: 594 540 15 Fragments arranged by size: 594 540 15 NgoMIV G'CCGG_C Cuts at: 0 592 1132 1149 Size: 592 540 17 Fragments arranged by size: 592 540 17 NspI r_CATG'y Cuts at: 0 1026 1149 Size: 1026 123 PasI CC'CwG_GG Cuts at: 0 808 1149 Size: 808 341 PpuMI rG'GwC_Cy Cuts at: 0 151 302 1071 1149 Size: 151 151 769 78 Fragments arranged by size: 769 151 151 78 PspOMI G'GGCC_C Cuts at: 0 385 1149 Size: 385 764 PstI C_TGCA'G Cuts at: 0 616 674 1149 Size: 616 58 475 Fragments arranged by size: 616 475 58 PvuII CAG'CTG Cuts at: 0 518 1149 Size: 518 631 SacI G_AGCT'C Cuts at: 0 251 875 1149 Size: 251 624 274 Fragments arranged by size: 624 274 251 SanDI GG'GwC_CC Cuts at: 0 151 1149 Size: 151 998 SfcI C'TryA_G Cuts at: 0 612 670 1149 Size: 612 58 479 Fragments arranged by size: 612 479 58 SmaI CCC'GGG Cuts at: 0 213 351 1149 Size: 213 138 798 Fragments arranged by size: 798 213 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 25 694 987 1149 Size: 25 669 293 162 Fragments arranged by size: 669 293 162 25 TatI w'GTAC_w Cuts at: 0 1055 1149 Size: 1055 94 Tth111I GACn'n_nGTC Cuts at: 0 547 1149 Size: 547 602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 61 1149 Size: 61 1088 XmaI C'CCGG_G Cuts at: 0 211 349 1149 Size: 211 138 800 Fragments arranged by size: 800 211 138 Enzymes that do cut: AarI AcuI AleI AlfI ApaI ApaLI AvaI BanI BanII BbsI BcgI BclI BglI BlpI Bme1580I BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI BtsI DrdI EaeI EcoICRI Eco57MI EcoNI EcoO109I HincII MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PpuMI PspOMI PstI PvuII SacI SanDI SfcI SmaI TaqII TatI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BfrBI BglII BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EagI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TstI XbaI XhoI XmnI ZraI