################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BbrPI BbvCI BciVI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoT22I FseI FspI KpnI KspI MaeI MaeII MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccII 793 CGCG CG^CG AccIII 158 TCCGGA T^CCGGA AfaI 648 GTAC GT^AC AlwNI 50 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1207 AGCGCT AGC^GCT ApaI 418 GGGCCC GGGCC^C AspI 1686 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1388 TGGCCA TGG^CCA BanI 117 GGCGCC G^G[CT][GA]CC BanII 418 GGGCCC G[GA]GC[CT]^C BbeI 117 GGCGCC GGCGC^C BcgI 900 GCAGGATTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 848 TGATCA T^GATCA BcnI 1455 CCGGG CC^[GC]GG BglI 1051 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1135 GCTCAGC GC^T[ACGT]AGC BmyI 418 GGGCCC G[AGT]GC[ACT]^C Bpu1102I 1135 GCTCAGC GC^T[ACGT]AGC BsaWI 158 TCCGGA [AT]^CCGG[AT] BseAI 158 TCCGGA T^CCGGA Bsp1286I 418 GGGCCC G[AGT]GC[ACT]^C BspEI 158 TCCGGA T^CCGGA BsrDI 1106 CATTGC GCAATG(2/0) BsrFI 1078 GCCGGC [GA]^CCGG[CT] BssSI 1073 CTCGTG CACGAG(-5/-1) BstUI 793 CGCG CG^CG CelII 1135 GCTCAGC GC^T[ACGT]AGC Cfr10I 1078 GCCGGC [GA]^CCGG[CT] DraI 1311 TTTAAA TTT^AAA DrdI 1261 GACAACGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 452 CCATGG C^C[GA][CT]GG EaeI 1388 TGGCCA [CT]^GGCC[GA] Eco47III 1207 AGCGCT AGC^GCT EcoRV 337 GATATC GAT^ATC FauI 791 CCCGC CCCGC(4/6) FbaI 848 TGATCA T^GATCA HgaI 1738 GACGC GACGC(5/10) HindIII 1018 AAGCTT A^AGCTT HpaI 295 GTTAAC GTT^AAC KasI 117 GGCGCC G^GCGCC MluNI 1388 TGGCCA TGG^CCA MroI 158 TCCGGA T^CCGGA MscI 1388 TGGCCA TGG^CCA MslI 679 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 793 CGCG CG^CG NaeI 1078 GCCGGC GCC^GGC NarI 117 GGCGCC GG^CGCC NciI 1455 CCGGG CC^[GC]GG NcoI 452 CCATGG C^CATGG NgoMIV 1078 GCCGGC G^CCGGC PflFI 1686 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1470 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1445 CAGCTG CAG^CTG RsaI 648 GTAC GT^AC SexAI 1687 ACCAGGT A^CC[AT]GGT SfoI 117 GGCGCC GGC^GCC Tth111I 1686 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1470 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 127 CCAGGAAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 117 GGCGCC G[GA]^CG[CT]C 1738 GACGCC AvaI 1548 CTCGAG C^[CT]CG[GA]G 1576 CTCGAG AvaII 1685 GGACC G^G[AT]CC 1691 GGTCC BbsI 1735 GAAGAC GAAGAC(2/6) 616 GTCTTC BpuAI 1735 GAAGAC GAAGAC(2/6) 616 GTCTTC BsaHI 117 GGCGCC G[GA]^CG[CT]C 1738 GACGCC BsoBI 1548 CTCGAG C^[CT]CG[GA]G 1576 CTCGAG BstYI 155 AGATCC [GA]^GATC[CT] 1747 GGATCT DraII 417 GGGGCCC [GA]G^G[ACGT]CC[CT] 418 GGGCCCT EcoNI 121 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1575 CCTCGAGGAGG EcoO109I 417 GGGGCCC [GA]G^G[ACGT]CC[CT] 418 GGGCCCT HaeII 117 GGCGCC [GA]GCGC^[CT] 1207 AGCGCT Hin1I 117 GGCGCC G[GA]^CG[CT]C 1738 GACGCC HincII 295 GTTAAC GT[CT]^[GA]AC 1450 GTCAAC HindII 295 GTTAAC GT[CT]^[GA]AC 1450 GTCAAC MflI 155 AGATCC [GA]^GATC[CT] 1747 GGATCT MspA1I 879 CCGCTG C[AC]G^C[GT]G 1445 CAGCTG PaeR7I 1548 CTCGAG C^TCGAG 1576 CTCGAG PstI 97 CTGCAG CTGCA^G 898 CTGCAG SfaNI 1600 GATGC GCATC(5/9) 1082 GCATC XhoI 1548 CTCGAG C^TCGAG 1576 CTCGAG XhoII 155 AGATCC [GA]^GATC[CT] 1747 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbvI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I