(Circular) (Six-base) MAPSORT of: pFN21AE1165.seq Check: 7365 from: 1 to: 6487 pFN21AE1165 6487 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4297 278 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3830 1937 Size: 1893 4594 Acc65I G'GTAC_C Cuts at: 3808 3808 Size: 6487 AclI AA'CG_TT Cuts at: 1563 4614 4987 1563 Size: 3051 373 3063 Fragments arranged by size: 3063 3051 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2579 2608 3198 3744 4550 5934 6202 1632 Size: 947 29 590 546 806 1384 268 1917 Fragments arranged by size: 1917 1384 947 806 590 546 268 29 AfeI AGC'GCT Cuts at: 3214 3214 Size: 6487 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6290 AflIII A'CryG_T Cuts at: 1203 5386 6363 1203 Size: 4183 977 1327 Fragments arranged by size: 4183 1327 977 AgeI A'CCGG_T Cuts at: 5318 5318 Size: 6487 AhdI GACnn_n'nnGTC Cuts at: 5216 5216 Size: 6487 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6487 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6487 AlwNI CAG_nnn'CTG Cuts at: 2060 4230 5802 2060 Size: 2170 1572 2745 Fragments arranged by size: 2745 2170 1572 ApaI G_GGCC'C Cuts at: 2427 2427 Size: 6487 ApaLI G'TGCA_C Cuts at: 4544 5700 4544 Size: 1156 5331 ApoI r'AATT_y Cuts at: 1493 2410 2775 3248 3796 4022 4174 6283 1493 Size: 917 365 473 548 226 152 2109 1697 Fragments arranged by size: 2109 1697 917 548 473 365 226 152 AseI AT'TA_AT Cuts at: 160 1032 5041 160 Size: 872 4009 1606 Fragments arranged by size: 4009 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 AvaI C'yCGr_G Cuts at: 1870 1943 3553 3581 3812 1870 Size: 73 1610 28 231 4545 Fragments arranged by size: 4545 1610 231 73 28 BamHI G'GATC_C Cuts at: 3817 4184 3817 Size: 367 6120 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 3808 5263 618 Size: 325 217 627 335 1686 1455 1842 Fragments arranged by size: 1842 1686 1455 627 335 325 217 BanII G_rGCy'C Cuts at: 1391 2427 3806 1391 Size: 1036 1379 4072 Fragments arranged by size: 4072 1379 1036 BbeI G_GCGC'C Cuts at: 2126 2126 Size: 6487 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2614 3747 928 Size: 971 715 1133 3668 Fragments arranged by size: 3668 1133 971 715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2894 2928 4678 4712 1903 Size: 34 957 34 1750 34 3678 Fragments arranged by size: 3678 1750 957 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4379 5589 4379 Size: 1210 5277 BclI T'GATC_A Cuts at: 1548 2853 1548 Size: 1305 5182 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3062 5098 136 Size: 107 122 71 631 605 1390 2036 1525 Fragments arranged by size: 2036 1525 1390 631 605 122 107 71 BglII A'GATC_T Cuts at: 6482 6482 Size: 6487 BlpI GC'TnA_GC Cuts at: 3141 3904 3141 Size: 763 5724 Bme1580I G_kGCm'C Cuts at: 1790 2427 4548 5704 1790 Size: 637 2121 1156 2573 Fragments arranged by size: 2573 2121 1156 637 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6487 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2538 4218 5176 Size: 416 53 83 399 24 1121 1680 958 Cuts at: 5176 442 Size: 1753 Fragments arranged by size: 1753 1680 1121 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6487 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2940 3615 3795 5147 1239 Size: 1701 675 180 1352 2579 Fragments arranged by size: 2579 1701 1352 675 180 Bpu10I CC'TnA_GC Cuts at: 3409 3409 Size: 6487 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2421 3592 4612 5477 5775 2421 Size: 1171 1020 865 298 3133 Fragments arranged by size: 3133 1171 1020 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5150 882 Size: 617 3651 2219 Fragments arranged by size: 3651 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4278 493 Size: 711 3074 2702 Fragments arranged by size: 3074 2702 711 BsaBI GATnn'nnATC Cuts at: 6481 6481 Size: 6487 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2123 3744 4294 4676 Size: 53 83 186 936 590 1621 550 382 Cuts at: 4676 5311 275 Size: 635 1451 Fragments arranged by size: 1621 1451 936 635 590 550 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2163 4920 5318 5592 5739 6150 2163 Size: 2757 398 274 147 411 2500 Fragments arranged by size: 2757 2500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6262 Fragments arranged by size: 6262 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2550 3599 3602 1186 Size: 1364 1049 3 4071 Fragments arranged by size: 4071 1364 1049 3 BseYI C'CCAG_C Cuts at: 3202 3261 5690 6186 3202 Size: 59 2429 496 3503 Fragments arranged by size: 3503 2429 496 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 3468 3620 1163 Size: 573 441 1291 152 4030 Fragments arranged by size: 4030 1291 573 441 152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3937 4698 5726 1540 Size: 275 186 1936 761 1028 2301 Fragments arranged by size: 2301 1936 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4548 4633 5704 4548 Size: 85 1071 5331 Fragments arranged by size: 5331 1071 85 BsmI GAATG_Cn' Cuts at: 3998 4091 3998 Size: 93 6394 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2427 3806 4548 4633 5704 1391 Size: 399 637 1379 742 85 1071 2174 Fragments arranged by size: 2174 1379 1071 742 637 399 85 BspEI T'CCGG_A Cuts at: 2163 6150 2163 Size: 3987 2500 BspHI T'CATG_A Cuts at: 4376 6106 4376 Size: 1730 4757 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3842 844 Size: 2998 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4374 1394 Size: 429 2551 3507 Fragments arranged by size: 3507 2551 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3110 4982 5156 65 Size: 1183 1862 1872 174 1396 Fragments arranged by size: 1872 1862 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3083 5131 5318 1700 Size: 110 1273 2048 187 2869 Fragments arranged by size: 2869 2048 1273 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6487 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6487 BssSI C'ACGA_G Cuts at: 3078 4547 5559 3078 Size: 1469 1012 4006 Fragments arranged by size: 4006 1469 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6487 BstBI TT'CG_AA Cuts at: 5291 5291 Size: 6487 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6487 BstYI r'GATC_y Cuts at: 1972 2160 3752 3817 4184 4569 4586 6038 6124 Size: 188 1592 65 367 385 17 1452 86 Cuts at: 6124 6136 6482 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1592 1452 385 367 346 188 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2457 513 Size: 552 939 453 4543 Fragments arranged by size: 4543 939 552 453 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2452 487 Size: 683 56 1226 4522 Fragments arranged by size: 4522 1226 683 56 BtsI GCAGTG_nn' Cuts at: 3768 4003 4797 4817 3768 Size: 235 794 20 5438 Fragments arranged by size: 5438 794 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6487 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2364 2399 529 Size: 35 1800 35 4617 Fragments arranged by size: 4617 1800 35 35 DraI TTT'AAA Cuts at: 3318 3791 3318 Size: 473 6014 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3272 5494 809 Size: 507 1956 2222 1802 Fragments arranged by size: 2222 1956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3393 3934 4823 Size: 54 1425 50 275 18 1563 541 889 Cuts at: 4823 6452 8 Size: 1629 43 Fragments arranged by size: 1629 1563 1425 889 541 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3934 1537 Size: 275 2122 4090 Fragments arranged by size: 4090 2122 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2009 2142 2307 2755 3507 4417 1353 Size: 656 133 165 448 752 910 3423 Fragments arranged by size: 3423 910 752 656 448 165 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5074 5448 5594 387 Size: 1064 138 3485 374 146 1280 Fragments arranged by size: 3485 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2579 2608 2940 3198 3615 3744 3795 Size: 393 947 29 332 258 417 129 51 Cuts at: 3795 4550 5147 5934 6202 1239 Size: 755 597 787 268 1524 Fragments arranged by size: 1524 947 787 755 597 417 393 332 268 258 129 51 29 EcoNI CCTnn'n_nnAGG Cuts at: 2130 3584 2130 Size: 1454 5033 EcoO109I rG'GnC_Cy Cuts at: 2423 2424 2423 Size: 1 6486 EcoRI G'AATT_C Cuts at: 3796 4174 3796 Size: 378 6109 EcoRV GAT'ATC Cuts at: 2344 2344 Size: 6487 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 6487 FspI TGC'GCA Cuts at: 4993 4993 Size: 6487 HaeII r_GCGC'y Cuts at: 1678 2126 3216 5634 1678 Size: 448 1090 2418 2531 Fragments arranged by size: 2531 2418 1090 448 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2152 2184 2671 2703 2794 Size: 32 135 32 580 32 487 32 91 Cuts at: 2794 2826 2984 3016 3290 3322 3612 3644 5128 Size: 32 158 32 274 32 290 32 1484 Cuts at: 5128 5160 5202 5234 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1484 580 487 290 274 158 135 91 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2302 3457 3831 4077 669 Size: 988 281 364 1155 374 246 3079 Fragments arranged by size: 3079 1155 988 374 364 281 246 HindIII A'AGCT_T Cuts at: 748 3023 748 Size: 2275 4212 HpaI GTT'AAC Cuts at: 2302 4077 2302 Size: 1775 4712 KasI G'GCGC_C Cuts at: 2122 2122 Size: 6487 KpnI G_GTAC'C Cuts at: 3812 3812 Size: 6487 MfeI C'AATT_G Cuts at: 4086 4086 Size: 6487 MluI A'CGCG_T Cuts at: 6363 6363 Size: 6487 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2979 3513 5601 5785 1305 Size: 1674 534 2088 184 2007 Fragments arranged by size: 2088 2007 1674 534 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3395 10 Size: 54 1425 343 1563 3102 Fragments arranged by size: 3102 1563 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2688 4445 4804 4963 518 Size: 648 1522 1757 359 159 2042 Fragments arranged by size: 2042 1757 1522 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2886 3452 3903 4580 5728 5973 1606 Size: 1280 566 451 677 1148 245 2120 Fragments arranged by size: 2120 1280 1148 677 566 451 245 NaeI GCC'GGC Cuts at: 3085 3085 Size: 6487 NarI GG'CG_CC Cuts at: 2123 2123 Size: 6487 NcoI C'CATG_G Cuts at: 513 1065 2004 2457 513 Size: 552 939 453 4543 Fragments arranged by size: 4543 939 552 453 NdeI CA'TA_TG Cuts at: 387 387 Size: 6487 NgoMIV G'CCGG_C Cuts at: 3083 3083 Size: 6487 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6487 NotI GC'GGCC_GC Cuts at: 3934 3934 Size: 6487 NspI r_CATG'y Cuts at: 1132 1229 3845 5390 1132 Size: 97 2616 1545 2229 Fragments arranged by size: 2616 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6487 PciI A'CATG_T Cuts at: 5386 5386 Size: 6487 PflMI CCAn_nnn'nTGG Cuts at: 1108 3481 1108 Size: 2373 4114 PmeI GTTT'AAAC Cuts at: 3791 3791 Size: 6487 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4556 4588 6069 6101 4556 Size: 32 1481 32 4942 Fragments arranged by size: 4942 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6487 PsiI TTA'TAA Cuts at: 4057 4057 Size: 6487 PspOMI G'GGCC_C Cuts at: 2423 2423 Size: 6487 PspXI vC'TCGA_Gb Cuts at: 3553 3581 3553 Size: 28 6459 PstI C_TGCA'G Cuts at: 830 2106 2907 3839 830 Size: 1276 801 932 3478 Fragments arranged by size: 3478 1276 932 801 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6487 PvuII CAG'CTG Cuts at: 3452 3452 Size: 6487 SalI G'TCGA_C Cuts at: 1936 3829 1936 Size: 1893 4594 SbfI CC_TGCA'GG Cuts at: 3839 3839 Size: 6487 ScaI AGT'ACT Cuts at: 4735 6447 4735 Size: 1712 4775 SexAI A'CCwGG_T Cuts at: 3692 3692 Size: 6487 SfcI C'TryA_G Cuts at: 826 1045 2052 2102 2734 2903 3835 4970 5651 Size: 219 1007 50 632 169 932 1135 681 Cuts at: 5651 5842 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1007 932 681 632 219 191 169 50 SfoI GGC'GCC Cuts at: 2124 2124 Size: 6487 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6487 SmaI CCC'GGG Cuts at: 1872 3814 1872 Size: 1942 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2436 3553 3571 3581 4591 5492 Size: 197 926 493 1117 18 10 1010 901 Cuts at: 5492 5754 820 Size: 262 1553 Fragments arranged by size: 1553 1117 1010 926 901 493 262 197 18 10 SnaBI TAC'GTA Cuts at: 493 4278 493 Size: 3785 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6487 SphI G_CATG'C Cuts at: 1132 3845 1132 Size: 2713 3774 SspI AAT'ATT Cuts at: 5 52 4411 5 Size: 47 4359 2081 Fragments arranged by size: 4359 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6487 StyI C'CwwG_G Cuts at: 513 1065 2004 2457 3234 3523 3926 513 Size: 552 939 453 777 289 403 3074 Fragments arranged by size: 3074 939 777 552 453 403 289 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2457 4514 4531 4684 4869 5314 325 Size: 1255 877 2057 17 153 185 445 1498 Fragments arranged by size: 2057 1498 1255 877 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4733 6445 96 Size: 274 80 33 51 1443 2756 1712 138 Fragments arranged by size: 2756 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2723 2755 6447 6479 2723 Size: 32 3692 32 2731 Fragments arranged by size: 3692 2731 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3694 1316 Size: 2378 4109 XbaI T'CTAG_A Cuts at: 3823 3823 Size: 6487 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2139 2139 Size: 6487 XhoI C'TCGA_G Cuts at: 1943 3553 3581 1943 Size: 1610 28 4849 Fragments arranged by size: 4849 1610 28 XmaI C'CCGG_G Cuts at: 1870 3812 1870 Size: 1942 4545 XmnI GAAnn'nnTTC Cuts at: 4616 4616 Size: 6487 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4295 276 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI