(Linear) (Six-base) MAPSORT of: KIEE1165.seq Check: 5549 from: 1 to: 1782 KIEE1165.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 603 1193 1739 1782 Size: 574 29 590 546 43 Fragments arranged by size: 590 574 546 43 29 AfeI AGC'GCT Cuts at: 0 1209 1782 Size: 1209 573 AlwNI CAG_nnn'CTG Cuts at: 0 55 1782 Size: 55 1727 ApaI G_GGCC'C Cuts at: 0 422 1782 Size: 422 1360 ApoI r'AATT_y Cuts at: 0 405 770 1243 1782 Size: 405 365 473 539 Fragments arranged by size: 539 473 405 365 AvaI C'yCGr_G Cuts at: 0 1548 1576 1782 Size: 1548 28 206 Fragments arranged by size: 1548 206 28 BanI G'GyrC_C Cuts at: 0 117 1782 Size: 117 1665 BanII G_rGCy'C Cuts at: 0 422 1782 Size: 422 1360 BbeI G_GCGC'C Cuts at: 0 121 1782 Size: 121 1661 BbsI GAAGACnn'nnnn_ Cuts at: 0 609 1742 1782 Size: 609 1133 40 Fragments arranged by size: 1133 609 40 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 889 923 1782 Size: 889 34 859 Fragments arranged by size: 889 859 34 BclI T'GATC_A Cuts at: 0 848 1782 Size: 848 934 BglI GCCn_nnn'nGGC Cuts at: 0 1057 1782 Size: 1057 725 BlpI GC'TnA_GC Cuts at: 0 1136 1782 Size: 1136 646 Bme1580I G_kGCm'C Cuts at: 0 422 1782 Size: 422 1360 BmrI ACTGGGnnnn_n' Cuts at: 0 533 1782 Size: 533 1249 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 935 1610 1782 Size: 935 675 172 Fragments arranged by size: 935 675 172 Bpu10I CC'TnA_GC Cuts at: 0 1404 1782 Size: 1404 378 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 416 1587 1782 Size: 416 1171 195 Fragments arranged by size: 1171 416 195 BsaHI Gr'CG_yC Cuts at: 0 118 1739 1782 Size: 118 1621 43 Fragments arranged by size: 1621 118 43 BsaWI w'CCGG_w Cuts at: 0 158 1782 Size: 158 1624 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 545 1594 1597 1782 Size: 545 1049 3 185 Fragments arranged by size: 1049 545 185 3 BseYI C'CCAG_C Cuts at: 0 1197 1256 1782 Size: 1197 59 526 Fragments arranged by size: 1197 526 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 1463 1615 1782 Size: 172 1291 152 167 Fragments arranged by size: 1291 172 167 152 Bsp1286I G_dGCh'C Cuts at: 0 422 1782 Size: 422 1360 BspEI T'CCGG_A Cuts at: 0 158 1782 Size: 158 1624 BsrDI GCAATG_nn' Cuts at: 0 1105 1782 Size: 1105 677 BsrFI r'CCGG_y Cuts at: 0 1078 1782 Size: 1078 704 BssSI C'ACGA_G Cuts at: 0 1073 1782 Size: 1073 709 BstYI r'GATC_y Cuts at: 0 155 1747 1782 Size: 155 1592 35 Fragments arranged by size: 1592 155 35 BtgI C'CryG_G Cuts at: 0 452 1782 Size: 452 1330 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 447 1782 Size: 447 1335 BtsI GCAGTG_nn' Cuts at: 0 1763 1782 Size: 1763 19 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 359 394 1782 Size: 359 35 1388 Fragments arranged by size: 1388 359 35 DraI TTT'AAA Cuts at: 0 1313 1782 Size: 1313 469 DrdI GACnn_nn'nnGTC Cuts at: 0 1267 1782 Size: 1267 515 EaeI y'GGCC_r Cuts at: 0 1388 1782 Size: 1388 394 EarI CTCTTCn'nnn_ Cuts at: 0 4 137 302 750 1502 1782 Size: 4 133 165 448 752 280 Fragments arranged by size: 752 448 280 165 133 4 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 603 935 1193 1610 1739 1782 Size: 574 29 332 258 417 129 43 Fragments arranged by size: 574 417 332 258 129 43 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 125 1579 1782 Size: 125 1454 203 Fragments arranged by size: 1454 203 125 EcoO109I rG'GnC_Cy Cuts at: 0 418 419 1782 Size: 418 1 1363 Fragments arranged by size: 1363 418 1 EcoRV GAT'ATC Cuts at: 0 339 1782 Size: 339 1443 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 90 1782 Size: 90 1692 HaeII r_GCGC'y Cuts at: 0 121 1211 1782 Size: 121 1090 571 Fragments arranged by size: 1090 571 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 147 179 666 698 789 821 979 1011 Size: 147 32 487 32 91 32 158 32 Cuts at: 1011 1285 1317 1607 1639 1782 Size: 274 32 290 32 143 Fragments arranged by size: 487 290 274 158 147 143 91 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 297 1452 1782 Size: 297 1155 330 Fragments arranged by size: 1155 330 297 HindIII A'AGCT_T Cuts at: 0 1018 1782 Size: 1018 764 HpaI GTT'AAC Cuts at: 0 297 1782 Size: 297 1485 KasI G'GCGC_C Cuts at: 0 117 1782 Size: 117 1665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 974 1508 1782 Size: 974 534 274 Fragments arranged by size: 974 534 274 MscI TGG'CCA Cuts at: 0 1390 1782 Size: 1390 392 MslI CAynn'nnrTG Cuts at: 0 683 1782 Size: 683 1099 MspA1I CmG'CkG Cuts at: 0 881 1447 1782 Size: 881 566 335 Fragments arranged by size: 881 566 335 NaeI GCC'GGC Cuts at: 0 1080 1782 Size: 1080 702 NarI GG'CG_CC Cuts at: 0 118 1782 Size: 118 1664 NcoI C'CATG_G Cuts at: 0 452 1782 Size: 452 1330 NgoMIV G'CCGG_C Cuts at: 0 1078 1782 Size: 1078 704 PflMI CCAn_nnn'nTGG Cuts at: 0 1476 1782 Size: 1476 306 PspOMI G'GGCC_C Cuts at: 0 418 1782 Size: 418 1364 PspXI vC'TCGA_Gb Cuts at: 0 1548 1576 1782 Size: 1548 28 206 Fragments arranged by size: 1548 206 28 PstI C_TGCA'G Cuts at: 0 101 902 1782 Size: 101 801 880 Fragments arranged by size: 880 801 101 PvuII CAG'CTG Cuts at: 0 1447 1782 Size: 1447 335 SexAI A'CCwGG_T Cuts at: 0 1687 1782 Size: 1687 95 SfcI C'TryA_G Cuts at: 0 47 97 729 898 1782 Size: 47 50 632 169 884 Fragments arranged by size: 884 632 169 50 47 SfoI GGC'GCC Cuts at: 0 119 1782 Size: 119 1663 SmlI C'TyrA_G Cuts at: 0 431 1548 1566 1576 1782 Size: 431 1117 18 10 206 Fragments arranged by size: 1117 431 206 18 10 StyI C'CwwG_G Cuts at: 0 452 1229 1518 1782 Size: 452 777 289 264 Fragments arranged by size: 777 452 289 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 452 1782 Size: 452 1330 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 718 750 1782 Size: 718 32 1032 Fragments arranged by size: 1032 718 32 Tth111I GACn'n_nGTC Cuts at: 0 1689 1782 Size: 1689 93 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 134 1782 Size: 134 1648 XhoI C'TCGA_G Cuts at: 0 1548 1576 1782 Size: 1548 28 206 Fragments arranged by size: 1548 206 28 Enzymes that do cut: AcuI AfeI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BcgI BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsgI Bsp1286I BspEI BsrDI BsrFI BssSI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PflMI PspOMI PspXI PstI PvuII SexAI SfcI SfoI SmlI StyI TaqII TstI Tth111I XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BglII BmgBI BmtI BplI BsaI BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraIII EagI EciI EcoICRI EcoRI FseI FspI FspAI KpnI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XmaI XmnI ZraI