################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI BbvCI BciVI BfrI BglII BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I HindIII HpaI KpnI MfeI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 2653 GACGTC GACGT^C AccI 2593 GTCTAC GT^[AC][GT]AC AcsI 840 GAATTT [GA]^AATT[CT] ApoI 840 GAATTT [GA]^AATT[CT] Asp700I 1176 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1036 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 875 TGCGCA TGC^GCA AvrII 1809 CCTAGG C^CTAGG BamHI 347 GGATCC G^GATCC BbeI 1861 GGCGCC GGCGC^C BbrPI 2388 CACGTG CAC^GTG BclI 497 TGATCA T^GATCA BfaI 1810 CTAG C^TAG BlnI 1809 CCTAGG C^CTAGG BlpI 1683 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1683 GCTGAGC GC^T[ACGT]AGC BsaAI 2388 CACGTG [CT]AC^GT[GA] BsaBI 342 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 570 GGTCTC GGTCTC(1/5) BsgI 432 GTGCAG GTGCAG(16/14) BsmAI 571 GTCTC GTCTC(1/5) BsrDI 420 CATTGC GCAATG(2/0) BstYI 347 GGATCC [GA]^GATC[CT] CelII 1683 GCTGAGC GC^T[ACGT]AGC EcoNI 276 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 497 TGATCA T^GATCA FspI 875 TGCGCA TGC^GCA HincII 1951 GTCAAC GT[CT]^[GA]AC HindII 1951 GTCAAC GT[CT]^[GA]AC KasI 1861 GGCGCC G^GCGCC MaeI 1810 CTAG C^TAG MamI 342 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 347 GGATCC [GA]^GATC[CT] NarI 1861 GGCGCC GG^CGCC PflFI 1036 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 966 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2388 CACGTG CAC^GTG PmlI 2388 CACGTG CAC^GTG PshAI 97 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2001 GCTCTTC GCTCTTC(1/4) ScaI 2396 AGTACT AGT^ACT SfoI 1861 GGCGCC GGC^GCC StuI 436 AGGCCT AGG^CCT Tsp509I 841 AATT ^AATT Tth111I 1036 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 966 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 347 GGATCC [GA]^GATC[CT] XmnI 1176 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Alw44I 1330 GTGCAC G^TGCAC 2437 GTGCAC AlwI 348 GATCC GGATC(4/5) 347 GGATC AlwNI 59 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 2128 CAGTGGCTG ApaI 885 GGGCCC GGGCC^C 2346 GGGCCC ApaLI 1330 GTGCAC G^TGCAC 2437 GTGCAC BalI 174 TGGCCA TGG^CCA 1061 TGGCCA BcgI 1319 GCAATCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2194 GCACGACAGTCG BspHI 836 TCATGA T^CATGA 2162 TCATGA BssSI 1558 CACGAG CACGAG(-5/-1) 1582 CACGAG BstAPI 668 GCACAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1277 GCAAGTCGTGC BstXI 1505 CCAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1751 CCAAGGCGCTGG EarI 946 CTCTTC CTCTTC(1/4) 2002 CTCTTC FseI 758 GGCCGGCC GGCCGG^CC 1452 GGCCGGCC Ksp632I 946 CTCTTC CTCTTC(1/4) 2002 CTCTTC KspI 781 CCGCGG CCGC^GG 1149 CCGCGG MluI 1385 ACGCGT A^CGCGT 2492 ACGCGT MluNI 174 TGGCCA TGG^CCA 1061 TGGCCA MscI 174 TGGCCA TGG^CCA 1061 TGGCCA MslI 1021 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2061 CATCAAGGTG NcoI 1058 CCATGG C^CATGG 1079 CCATGG NspI 673 ACATGC [GA]CATG^[CT] 1430 ACATGC PvuII 445 CAGCTG CAG^CTG 1372 CAGCTG RcaI 836 TCATGA T^CATGA 2162 TCATGA SacII 781 CCGCGG CCGC^GG 1149 CCGCGG SfiI 428 GGCCGTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2287 GGCCTCTCGGGCC SmlI 1408 CTCAAG C^T[CT][GA]AG 1528 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SgrAI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I XmaCI XmaI