(Circular) (Six-base) MAPSORT of: pFN21AE2514.seq Check: 455 from: 1 to: 7387 pFN21AE2514 7387 bp With 182 enzymes: SgfI * March 20, 2014 17:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2391 2391 Size: 7387 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4662 5197 278 Size: 53 83 186 936 3126 535 2468 Fragments arranged by size: 3126 2468 936 535 186 83 53 AccI GT'mk_AC Cuts at: 1937 4599 4730 1937 Size: 2662 131 4594 Fragments arranged by size: 4594 2662 131 Acc65I G'GTAC_C Cuts at: 4708 4708 Size: 7387 AclI AA'CG_TT Cuts at: 1563 5514 5887 1563 Size: 3951 373 3063 Fragments arranged by size: 3951 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2938 3655 3896 4576 5450 6834 7102 1632 Size: 1306 717 241 680 874 1384 268 1917 Fragments arranged by size: 1917 1384 1306 874 717 680 268 241 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7190 AflIII A'CryG_T Cuts at: 1203 3390 4122 4392 4497 6286 7263 1203 Size: 2187 732 270 105 1789 977 1327 Fragments arranged by size: 2187 1789 1327 977 732 270 105 AgeI A'CCGG_T Cuts at: 6218 6218 Size: 7387 AhdI GACnn_n'nnGTC Cuts at: 6116 6116 Size: 7387 AleI CACnn'nnGTG Cuts at: 1166 3030 1166 Size: 1864 5523 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2452 3048 3182 1184 Size: 1268 596 134 5389 Fragments arranged by size: 5389 1268 596 134 AlwNI CAG_nnn'CTG Cuts at: 2069 4138 5130 6702 2069 Size: 2069 992 1572 2754 Fragments arranged by size: 2754 2069 1572 992 ApaI G_GGCC'C Cuts at: 2894 4355 2894 Size: 1461 5926 ApaLI G'TGCA_C Cuts at: 3335 4442 5444 6600 3335 Size: 1107 1002 1156 4122 Fragments arranged by size: 4122 1156 1107 1002 ApoI r'AATT_y Cuts at: 1493 2845 4696 4922 5074 7183 1493 Size: 1352 1851 226 152 2109 1697 Fragments arranged by size: 2109 1851 1697 1352 226 152 AseI AT'TA_AT Cuts at: 160 1032 5941 160 Size: 872 4909 1606 Fragments arranged by size: 4909 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7387 AvaI C'yCGr_G Cuts at: 1870 1943 2578 2759 2810 2911 3148 3497 3659 Size: 73 635 181 51 101 237 349 162 Cuts at: 3659 3725 3733 4001 4040 4271 4297 4348 4355 Size: 66 8 268 39 231 26 51 7 Cuts at: 4355 4712 1870 Size: 357 4545 Fragments arranged by size: 4545 635 357 349 268 237 231 181 162 101 73 66 51 51 39 26 8 7 AvrII C'CTAG_G Cuts at: 3814 3814 Size: 7387 BamHI G'GATC_C Cuts at: 2352 4717 5084 2352 Size: 2365 367 4655 Fragments arranged by size: 4655 2365 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2261 3506 3721 3866 3986 Size: 325 217 627 474 1245 215 145 120 Cuts at: 3986 4708 6163 618 Size: 722 1455 1842 Fragments arranged by size: 1842 1455 1245 722 627 474 325 217 215 145 120 BanII G_rGCy'C Cuts at: 1391 2055 2322 2760 2894 3015 3480 3504 3843 Size: 664 267 438 134 121 465 24 339 Cuts at: 3843 3996 4008 4047 4355 4706 1391 Size: 153 12 39 308 351 4072 Fragments arranged by size: 4072 664 465 438 351 339 308 267 153 134 121 39 24 12 BbeI G_GCGC'C Cuts at: 3870 3870 Size: 7387 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2106 3434 4541 928 Size: 971 207 1328 1107 3774 Fragments arranged by size: 3774 1328 1107 971 207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3313 3347 4188 4222 5578 5612 1903 Size: 34 1376 34 841 34 1356 34 3678 Fragments arranged by size: 3678 1376 1356 841 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5279 6489 5279 Size: 1210 6177 BclI T'GATC_A Cuts at: 1548 2502 1548 Size: 954 6433 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2440 2812 2937 Size: 107 122 71 631 605 768 372 125 Cuts at: 2937 4299 4308 4461 5998 136 Size: 1362 9 153 1537 1525 Fragments arranged by size: 1537 1525 1362 768 631 605 372 153 125 122 107 71 9 BglII A'GATC_T Cuts at: 7382 7382 Size: 7387 BlpI GC'TnA_GC Cuts at: 3689 4804 3689 Size: 1115 6272 Bme1580I G_kGCm'C Cuts at: 1790 2524 2894 2942 3339 3726 4355 4446 5448 Size: 734 370 48 397 387 629 91 1002 Cuts at: 5448 6604 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1002 734 629 397 387 370 91 48 BmgBI CAC'GTC Cuts at: 1318 4221 1318 Size: 2903 4484 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3024 5118 6076 Size: 416 53 83 399 24 1607 2094 958 Cuts at: 6076 442 Size: 1753 Fragments arranged by size: 2094 1753 1607 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7387 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2774 3662 4106 4631 6047 1239 Size: 893 642 888 444 525 1416 2579 Fragments arranged by size: 2579 1416 893 888 642 525 444 Bpu10I CC'TnA_GC Cuts at: 2321 3215 2321 Size: 894 6493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3398 3518 5512 6377 6675 3398 Size: 120 1994 865 298 4110 Fragments arranged by size: 4110 1994 865 298 120 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2581 6050 882 Size: 617 1082 3469 2219 Fragments arranged by size: 3469 2219 1082 617 BsaAI yAC'GTr Cuts at: 493 1204 4395 5178 493 Size: 711 3191 783 2702 Fragments arranged by size: 3191 2702 783 711 BsaBI GATnn'nnATC Cuts at: 2351 7381 2351 Size: 5030 2357 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3867 4154 4659 5194 Size: 53 83 186 936 2334 287 505 535 Cuts at: 5194 5576 6211 275 Size: 382 635 1451 Fragments arranged by size: 2334 1451 936 635 535 505 382 287 186 83 53 BsaWI w'CCGG_w Cuts at: 5820 6218 6492 6639 7050 5820 Size: 398 274 147 411 6157 Fragments arranged by size: 6157 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2599 2629 1178 Size: 30 165 30 1196 30 5936 Fragments arranged by size: 5936 1196 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2296 2470 3017 3611 3614 3623 3626 Size: 949 161 174 547 594 3 9 3 Cuts at: 3626 3629 3632 3635 3638 3641 3644 4696 1186 Size: 3 3 3 3 3 3 1052 3877 Fragments arranged by size: 3877 1052 949 594 547 174 161 9 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2448 3021 3194 3580 3787 4034 4364 6590 7086 Size: 573 173 386 207 247 330 2226 496 Cuts at: 7086 2448 Size: 2749 Fragments arranged by size: 2749 2226 573 496 386 330 247 207 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2458 1163 Size: 573 722 6092 Fragments arranged by size: 6092 722 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3043 3463 3486 3543 4837 5598 Size: 275 186 1042 420 23 57 1294 761 Cuts at: 5598 6626 1540 Size: 1028 2301 Fragments arranged by size: 2301 1294 1042 1028 761 420 275 186 57 23 BsiHKAI G_wGCw'C Cuts at: 2055 2232 2322 3221 3339 3398 3843 4233 4446 Size: 177 90 899 118 59 445 390 213 Cuts at: 4446 4644 5448 5533 6604 2055 Size: 198 804 85 1071 2838 Fragments arranged by size: 2838 1071 899 804 445 390 213 198 177 118 90 85 59 BsmI GAATG_Cn' Cuts at: 4898 4991 4898 Size: 93 7294 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2232 2322 2524 2760 2894 2942 Size: 399 265 177 90 202 236 134 48 Cuts at: 2942 3015 3221 3339 3398 3480 3504 3726 3843 Size: 73 206 118 59 82 24 222 117 Cuts at: 3843 3996 4008 4047 4233 4355 4446 4644 4706 Size: 153 12 39 186 122 91 198 62 Cuts at: 4706 5448 5533 6604 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 265 236 222 206 202 198 186 177 153 134 122 118 117 91 90 85 82 73 62 59 48 39 24 12 BspEI T'CCGG_A Cuts at: 7050 7050 Size: 7387 BspHI T'CATG_A Cuts at: 2841 4167 5276 7006 2841 Size: 1326 1109 1730 3222 Fragments arranged by size: 3222 1730 1326 1109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 2391 2883 4287 4742 844 Size: 1331 216 492 1404 455 3489 Fragments arranged by size: 3489 1404 1331 492 455 216 BsrBI CCG'CTC Cuts at: 1394 1823 2809 2839 3465 4468 5274 1394 Size: 429 986 30 626 1003 806 3507 Fragments arranged by size: 3507 1003 986 806 626 429 30 BsrDI GCAATG_nn' Cuts at: 65 1248 2424 5882 6056 65 Size: 1183 1176 3458 174 1396 Fragments arranged by size: 3458 1396 1183 1176 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2338 2764 3171 3339 3446 3458 3654 Size: 110 528 426 407 168 107 12 196 Cuts at: 3654 4446 4460 6031 6218 1700 Size: 792 14 1571 187 2869 Fragments arranged by size: 2869 1571 792 528 426 407 196 187 168 110 107 14 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7387 BssHII G'CGCG_C Cuts at: 1925 2565 2567 4503 1925 Size: 640 2 1936 4809 Fragments arranged by size: 4809 1936 640 2 BssSI C'ACGA_G Cuts at: 3563 3587 5447 6459 3563 Size: 24 1860 1012 4491 Fragments arranged by size: 4491 1860 1012 24 BstAPI GCAn_nnn'nTGC Cuts at: 794 2679 3288 794 Size: 1885 609 4893 Fragments arranged by size: 4893 1885 609 BstBI TT'CG_AA Cuts at: 6191 6191 Size: 7387 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7387 BstXI CCAn_nnnn'nTGG Cuts at: 3517 3763 3517 Size: 246 7141 BstYI r'GATC_y Cuts at: 1972 2352 4717 5084 5469 5486 6938 7024 7036 Size: 380 2365 367 385 17 1452 86 12 Cuts at: 7036 7382 1972 Size: 346 1977 Fragments arranged by size: 2365 1977 1452 385 380 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2786 2823 3063 3084 3154 513 Size: 552 1721 37 240 21 70 4746 Fragments arranged by size: 4746 1721 552 240 70 37 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6648 Fragments arranged by size: 6648 683 56 BtsI GCAGTG_nn' Cuts at: 2249 3821 4139 4903 5697 5717 2249 Size: 1572 318 764 794 20 3919 Fragments arranged by size: 3919 1572 794 764 318 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7387 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7352 DraI TTT'AAA Cuts at: 4691 4691 Size: 7387 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6394 809 Size: 507 5078 1802 Fragments arranged by size: 5078 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2179 2766 3066 Size: 54 1425 50 275 18 349 587 300 Cuts at: 3066 3456 3460 3483 3540 3753 3846 4314 4834 Size: 390 4 23 57 213 93 468 520 Cuts at: 4834 5723 7352 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 587 520 468 390 349 300 275 213 93 57 54 50 43 23 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3460 3483 3540 4834 1537 Size: 275 1648 23 57 1294 4090 Fragments arranged by size: 4090 1648 1294 275 57 23 EarI CTCTTCn'nnn_ Cuts at: 1353 2957 4013 5317 1353 Size: 1604 1056 1304 3423 Fragments arranged by size: 3423 1604 1304 1056 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3545 4465 5974 6348 6494 387 Size: 1064 138 1956 920 1509 374 146 1280 Fragments arranged by size: 1956 1509 1280 1064 920 374 146 138 EcoICRI GAG'CTC Cuts at: 2053 2320 3841 2053 Size: 267 1521 5599 Fragments arranged by size: 5599 1521 267 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2774 2938 3655 3662 3896 4106 Size: 393 500 642 164 717 7 234 210 Cuts at: 4106 4576 4631 5450 6047 6834 7102 1239 Size: 470 55 819 597 787 268 1524 Fragments arranged by size: 1524 819 787 717 642 597 500 470 393 268 234 210 164 55 7 EcoNI CCTnn'n_nnAGG Cuts at: 2285 2285 Size: 7387 EcoO109I rG'GnC_Cy Cuts at: 2278 2891 3529 3730 4081 4174 4276 4352 2278 Size: 613 638 201 351 93 102 76 5313 Fragments arranged by size: 5313 638 613 351 201 102 93 76 EcoRI G'AATT_C Cuts at: 4696 5074 4696 Size: 378 7009 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2707 4195 2707 Size: 1488 5899 FseI GG_CCGG'CC Cuts at: 2768 3462 2768 Size: 694 6693 FspI TGC'GCA Cuts at: 2882 5893 2882 Size: 3011 4376 HaeII r_GCGC'y Cuts at: 1678 2532 3421 3764 3782 3870 4056 6534 1678 Size: 854 889 343 18 88 186 2478 2531 Fragments arranged by size: 2531 2478 889 854 343 186 88 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2599 2631 3464 3496 4220 Size: 32 135 32 1027 32 833 32 724 Cuts at: 4220 4252 6028 6060 6102 6134 1373 Size: 32 1776 32 42 32 2626 Fragments arranged by size: 2626 1776 1027 833 724 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3958 4731 4977 669 Size: 988 281 2020 773 246 3079 Fragments arranged by size: 3079 2020 988 773 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7387 HpaI GTT'AAC Cuts at: 4977 4977 Size: 7387 KasI G'GCGC_C Cuts at: 3866 3866 Size: 7387 KpnI G_GTAC'C Cuts at: 4712 4712 Size: 7387 MfeI C'AATT_G Cuts at: 4986 4986 Size: 7387 MluI A'CGCG_T Cuts at: 3390 4497 7263 3390 Size: 1107 2766 3514 Fragments arranged by size: 3514 2766 1107 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3492 6501 6685 1305 Size: 2187 3009 184 2007 Fragments arranged by size: 3009 2187 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2181 3068 10 Size: 54 1425 343 349 887 4329 Fragments arranged by size: 4329 1425 887 349 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3030 4070 5345 5704 5863 518 Size: 648 1864 1040 1275 359 159 2042 Fragments arranged by size: 2042 1864 1275 1040 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2452 2459 2788 3156 3229 3379 4030 4308 Size: 846 7 329 368 73 150 651 278 Cuts at: 4308 4320 4569 4803 5480 6628 6873 1606 Size: 12 249 234 677 1148 245 2120 Fragments arranged by size: 2120 1148 846 677 651 368 329 278 249 245 234 150 73 12 7 NaeI GCC'GGC Cuts at: 2766 3448 3460 3656 2766 Size: 682 12 196 6497 Fragments arranged by size: 6497 682 196 12 NarI GG'CG_CC Cuts at: 3867 3867 Size: 7387 NcoI C'CATG_G Cuts at: 513 1065 3063 3084 513 Size: 552 1998 21 4816 Fragments arranged by size: 4816 1998 552 21 NdeI CA'TA_TG Cuts at: 387 387 Size: 7387 NgoMIV G'CCGG_C Cuts at: 2764 3446 3458 3654 2764 Size: 682 12 196 6497 Fragments arranged by size: 6497 682 196 12 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7387 NotI GC'GGCC_GC Cuts at: 4834 4834 Size: 7387 NspI r_CATG'y Cuts at: 1132 1229 2682 3439 4745 6290 1132 Size: 97 1453 757 1306 1545 2229 Fragments arranged by size: 2229 1545 1453 1306 757 97 PasI CC'CwG_GG Cuts at: 1348 2524 1348 Size: 1176 6211 PciI A'CATG_T Cuts at: 6286 6286 Size: 7387 PflMI CCAn_nnn'nTGG Cuts at: 1108 2977 1108 Size: 1869 5518 PmeI GTTT'AAAC Cuts at: 4691 4691 Size: 7387 PmlI CAC'GTG Cuts at: 4395 4395 Size: 7387 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5456 5488 6969 7001 4112 Size: 32 1312 32 1481 32 4498 Fragments arranged by size: 4498 1481 1312 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3730 4081 4174 4276 3730 Size: 351 93 102 6841 Fragments arranged by size: 6841 351 102 93 PshAI GACnn'nnGTC Cuts at: 1613 2106 1613 Size: 493 6894 PsiI TTA'TAA Cuts at: 4957 4957 Size: 7387 PspOMI G'GGCC_C Cuts at: 2890 4351 2890 Size: 1461 5926 PstI C_TGCA'G Cuts at: 830 2564 2718 4430 4739 830 Size: 1734 154 1712 309 3478 Fragments arranged by size: 3478 1734 1712 309 154 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7387 PvuII CAG'CTG Cuts at: 2452 3379 2452 Size: 927 6460 SacI G_AGCT'C Cuts at: 2055 2322 3843 2055 Size: 267 1521 5599 Fragments arranged by size: 5599 1521 267 SacII CC_GC'GG Cuts at: 2789 3157 2789 Size: 368 7019 SalI G'TCGA_C Cuts at: 1936 4729 1936 Size: 2793 4594 SanDI GG'GwC_CC Cuts at: 3730 3730 Size: 7387 SapI GCTCTTCn'nnn_ Cuts at: 4013 4013 Size: 7387 SbfI CC_TGCA'GG Cuts at: 4739 4739 Size: 7387 ScaI AGT'ACT Cuts at: 4403 5635 7347 4403 Size: 1232 1712 4443 Fragments arranged by size: 4443 1712 1232 SfcI C'TryA_G Cuts at: 826 1045 2560 2714 4426 4600 4735 5870 6551 Size: 219 1515 154 1712 174 135 1135 681 Cuts at: 6551 6742 826 Size: 191 1471 Fragments arranged by size: 1712 1515 1471 1135 681 219 191 174 154 135 SfiI GGCCn_nnn'nGGCC Cuts at: 2440 4299 2440 Size: 1859 5528 SfoI GGC'GCC Cuts at: 3868 3868 Size: 7387 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7387 SgrAI Cr'CCGG_yG Cuts at: 1700 3339 4446 4460 1700 Size: 1639 1107 14 4627 Fragments arranged by size: 4627 1639 1107 14 SmaI CCC'GGG Cuts at: 1872 2761 2913 3150 3727 3735 4003 4042 4273 Size: 889 152 237 577 8 268 39 231 Cuts at: 4273 4350 4357 4714 1872 Size: 77 7 357 4545 Fragments arranged by size: 4545 889 577 357 268 237 231 152 77 39 8 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 3413 3533 5491 6392 6654 820 Size: 197 926 1470 120 1958 901 262 1553 Fragments arranged by size: 1958 1553 1470 926 901 262 197 120 SnaBI TAC'GTA Cuts at: 493 5178 493 Size: 4685 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7387 SphI G_CATG'C Cuts at: 1132 4745 1132 Size: 3613 3774 SrfI GCCC'GGGC Cuts at: 2761 4350 2761 Size: 1589 5798 SspI AAT'ATT Cuts at: 5 52 5311 5 Size: 47 5259 2081 Fragments arranged by size: 5259 2081 47 StuI AGG'CCT Cuts at: 1517 2443 1517 Size: 926 6461 StyI C'CwwG_G Cuts at: 513 1065 2202 3063 3084 3756 3814 4371 4826 Size: 552 1137 861 21 672 58 557 455 Cuts at: 4826 513 Size: 3074 Fragments arranged by size: 3074 1137 861 672 557 552 455 58 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 2249 2407 3057 5414 5431 5584 Size: 1255 558 111 158 650 2357 17 153 Cuts at: 5584 5769 6214 325 Size: 185 445 1498 Fragments arranged by size: 2357 1498 1255 650 558 445 185 158 153 111 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4125 4401 5633 Size: 274 80 33 51 1443 2148 276 1232 Cuts at: 5633 7345 96 Size: 1712 138 Fragments arranged by size: 2148 1712 1443 1232 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2230 2262 2581 2613 7347 7379 2230 Size: 32 319 32 4734 32 2238 Fragments arranged by size: 4734 2238 319 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3044 1316 Size: 1728 5659 XbaI T'CTAG_A Cuts at: 4723 4723 Size: 7387 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7387 XmaI C'CCGG_G Cuts at: 1870 2759 2911 3148 3725 3733 4001 4040 4271 Size: 889 152 237 577 8 268 39 231 Cuts at: 4271 4348 4355 4712 1870 Size: 77 7 357 4545 Fragments arranged by size: 4545 889 577 357 268 237 231 152 77 39 8 7 XmnI GAAnn'nnTTC Cuts at: 3185 5516 3185 Size: 2331 5056 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4660 5195 276 Size: 53 83 186 936 3126 535 2468 Fragments arranged by size: 3126 2468 936 535 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SexAI SwaI XcmI