(Linear) (Six-base) MAPSORT of: KIEE2514.seq Check: 7398 from: 1 to: 2682 KIEE2514.orf With 182 enzymes: SgfI * March 20, 2014 14:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 386 2682 Size: 386 2296 AatII G_ACGT'C Cuts at: 0 2657 2682 Size: 2657 25 AccI GT'mk_AC Cuts at: 0 2594 2682 Size: 2594 88 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 933 1650 1891 2571 2682 Size: 933 717 241 680 111 Fragments arranged by size: 933 717 680 241 111 AflIII A'CryG_T Cuts at: 0 1385 2117 2387 2492 2682 Size: 1385 732 270 105 190 Fragments arranged by size: 1385 732 270 190 105 AleI CACnn'nnGTG Cuts at: 0 1025 2682 Size: 1025 1657 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 447 1043 1177 2682 Size: 447 596 134 1505 Fragments arranged by size: 1505 596 447 134 AlwNI CAG_nnn'CTG Cuts at: 0 64 2133 2682 Size: 64 2069 549 Fragments arranged by size: 2069 549 64 ApaI G_GGCC'C Cuts at: 0 889 2350 2682 Size: 889 1461 332 Fragments arranged by size: 1461 889 332 ApaLI G'TGCA_C Cuts at: 0 1330 2437 2682 Size: 1330 1107 245 Fragments arranged by size: 1330 1107 245 ApoI r'AATT_y Cuts at: 0 840 2682 Size: 840 1842 AvaI C'yCGr_G Cuts at: 0 573 754 805 906 1143 1492 1654 1720 Size: 573 181 51 101 237 349 162 66 Cuts at: 1720 1728 1996 2035 2266 2292 2343 2350 2682 Size: 8 268 39 231 26 51 7 332 Fragments arranged by size: 573 349 332 268 237 231 181 162 101 66 51 51 39 26 8 7 AvrII C'CTAG_G Cuts at: 0 1809 2682 Size: 1809 873 BamHI G'GATC_C Cuts at: 0 347 2682 Size: 347 2335 BanI G'GyrC_C Cuts at: 0 256 1501 1716 1861 1981 2682 Size: 256 1245 215 145 120 701 Fragments arranged by size: 1245 701 256 215 145 120 BanII G_rGCy'C Cuts at: 0 50 317 755 889 1010 1475 1499 1838 Size: 50 267 438 134 121 465 24 339 Cuts at: 1838 1991 2003 2042 2350 2682 Size: 153 12 39 308 332 Fragments arranged by size: 465 438 339 332 308 267 153 134 121 50 39 24 12 BbeI G_GCGC'C Cuts at: 0 1865 2682 Size: 1865 817 BbsI GAAGACnn'nnnn_ Cuts at: 0 101 1429 2536 2682 Size: 101 1328 1107 146 Fragments arranged by size: 1328 1107 146 101 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1308 1342 2183 2217 2682 Size: 1308 34 841 34 465 Fragments arranged by size: 1308 841 465 34 34 BclI T'GATC_A Cuts at: 0 497 2682 Size: 497 2185 BglI GCCn_nnn'nGGC Cuts at: 0 435 807 932 2294 2303 2456 2682 Size: 435 372 125 1362 9 153 226 Fragments arranged by size: 1362 435 372 226 153 125 9 BlpI GC'TnA_GC Cuts at: 0 1684 2682 Size: 1684 998 Bme1580I G_kGCm'C Cuts at: 0 519 889 937 1334 1721 2350 2441 2682 Size: 519 370 48 397 387 629 91 241 Fragments arranged by size: 629 519 397 387 370 241 91 48 BmgBI CAC'GTC Cuts at: 0 2216 2682 Size: 2216 466 BmrI ACTGGGnnnn_n' Cuts at: 0 1019 2682 Size: 1019 1663 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 769 1657 2101 2626 2682 Size: 127 642 888 444 525 56 Fragments arranged by size: 888 642 525 444 127 56 Bpu10I CC'TnA_GC Cuts at: 0 316 1210 2682 Size: 316 894 1472 Fragments arranged by size: 1472 894 316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1393 1513 2682 Size: 1393 120 1169 Fragments arranged by size: 1393 1169 120 BsaI GGTCTCn'nnnn_ Cuts at: 0 576 2682 Size: 576 2106 BsaAI yAC'GTr Cuts at: 0 2390 2682 Size: 2390 292 BsaBI GATnn'nnATC Cuts at: 0 346 2682 Size: 346 2336 BsaHI Gr'CG_yC Cuts at: 0 1862 2149 2654 2682 Size: 1862 287 505 28 Fragments arranged by size: 1862 505 287 28 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 594 624 2682 Size: 594 30 2058 Fragments arranged by size: 2058 594 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 130 291 465 1012 1606 1609 1618 1621 Size: 130 161 174 547 594 3 9 3 Cuts at: 1621 1624 1627 1630 1633 1636 1639 2682 Size: 3 3 3 3 3 3 1043 Fragments arranged by size: 1043 594 547 174 161 130 9 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 443 1016 1189 1575 1782 2029 2359 2682 Size: 443 573 173 386 207 247 330 323 Fragments arranged by size: 573 443 386 330 323 247 207 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 453 2682 Size: 453 2229 BsiEI CG_ry'CG Cuts at: 0 1038 1458 1481 1538 2682 Size: 1038 420 23 57 1144 Fragments arranged by size: 1144 1038 420 57 23 BsiHKAI G_wGCw'C Cuts at: 0 50 227 317 1216 1334 1393 1838 2228 Size: 50 177 90 899 118 59 445 390 Cuts at: 2228 2441 2639 2682 Size: 213 198 43 Fragments arranged by size: 899 445 390 213 198 177 118 90 59 50 43 Bsp1286I G_dGCh'C Cuts at: 0 50 227 317 519 755 889 937 1010 Size: 50 177 90 202 236 134 48 73 Cuts at: 1010 1216 1334 1393 1475 1499 1721 1838 1991 Size: 206 118 59 82 24 222 117 153 Cuts at: 1991 2003 2042 2228 2350 2441 2639 2682 Size: 12 39 186 122 91 198 43 Fragments arranged by size: 236 222 206 202 198 186 177 153 134 122 118 117 91 90 82 73 59 50 48 43 39 24 12 BspHI T'CATG_A Cuts at: 0 836 2162 2682 Size: 836 1326 520 Fragments arranged by size: 1326 836 520 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 170 386 878 2282 2682 Size: 170 216 492 1404 400 Fragments arranged by size: 1404 492 400 216 170 BsrBI CCG'CTC Cuts at: 0 804 834 1460 2463 2682 Size: 804 30 626 1003 219 Fragments arranged by size: 1003 804 626 219 30 BsrDI GCAATG_nn' Cuts at: 0 419 2682 Size: 419 2263 BsrFI r'CCGG_y Cuts at: 0 333 759 1166 1334 1441 1453 1649 2441 Size: 333 426 407 168 107 12 196 792 Cuts at: 2441 2455 2682 Size: 14 227 Fragments arranged by size: 792 426 407 333 227 196 168 107 14 12 BssHII G'CGCG_C Cuts at: 0 560 562 2498 2682 Size: 560 2 1936 184 Fragments arranged by size: 1936 560 184 2 BssSI C'ACGA_G Cuts at: 0 1558 1582 2682 Size: 1558 24 1100 Fragments arranged by size: 1558 1100 24 BstAPI GCAn_nnn'nTGC Cuts at: 0 674 1283 2682 Size: 674 609 1399 Fragments arranged by size: 1399 674 609 BstXI CCAn_nnnn'nTGG Cuts at: 0 1512 1758 2682 Size: 1512 246 924 Fragments arranged by size: 1512 924 246 BstYI r'GATC_y Cuts at: 0 347 2682 Size: 347 2335 BtgI C'CryG_G Cuts at: 0 781 818 1058 1079 1149 2682 Size: 781 37 240 21 70 1533 Fragments arranged by size: 1533 781 240 70 37 21 BtsI GCAGTG_nn' Cuts at: 0 244 1816 2134 2682 Size: 244 1572 318 548 Fragments arranged by size: 1572 548 318 244 EaeI y'GGCC_r Cuts at: 0 174 761 1061 1451 1455 1478 1535 1748 Size: 174 587 300 390 4 23 57 213 Cuts at: 1748 1841 2309 2682 Size: 93 468 373 Fragments arranged by size: 587 468 390 373 300 213 174 93 57 23 4 EagI C'GGCC_G Cuts at: 0 1455 1478 1535 2682 Size: 1455 23 57 1147 Fragments arranged by size: 1455 1147 57 23 EarI CTCTTCn'nnn_ Cuts at: 0 952 2008 2682 Size: 952 1056 674 Fragments arranged by size: 1056 952 674 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1540 2460 2682 Size: 1540 920 222 Fragments arranged by size: 1540 920 222 EcoICRI GAG'CTC Cuts at: 0 48 315 1836 2682 Size: 48 267 1521 846 Fragments arranged by size: 1521 846 267 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 769 933 1650 1657 1891 2101 2571 Size: 127 642 164 717 7 234 210 470 Cuts at: 2571 2626 2682 Size: 55 56 Fragments arranged by size: 717 642 470 234 210 164 127 56 55 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 280 2682 Size: 280 2402 EcoO109I rG'GnC_Cy Cuts at: 0 273 886 1524 1725 2076 2169 2271 2347 Size: 273 613 638 201 351 93 102 76 Cuts at: 2347 2682 Size: 335 Fragments arranged by size: 638 613 351 335 273 201 102 93 76 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 702 2190 2682 Size: 702 1488 492 Fragments arranged by size: 1488 702 492 FseI GG_CCGG'CC Cuts at: 0 763 1457 2682 Size: 763 694 1225 Fragments arranged by size: 1225 763 694 FspI TGC'GCA Cuts at: 0 877 2682 Size: 877 1805 HaeII r_GCGC'y Cuts at: 0 527 1416 1759 1777 1865 2051 2682 Size: 527 889 343 18 88 186 631 Fragments arranged by size: 889 631 527 343 186 88 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 594 626 1459 1491 2215 2247 2682 Size: 594 32 833 32 724 32 435 Fragments arranged by size: 833 724 594 435 32 32 32 HincII GTy'rAC Cuts at: 0 1953 2682 Size: 1953 729 KasI G'GCGC_C Cuts at: 0 1861 2682 Size: 1861 821 MluI A'CGCG_T Cuts at: 0 1385 2492 2682 Size: 1385 1107 190 Fragments arranged by size: 1385 1107 190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1487 2682 Size: 1487 1195 MscI TGG'CCA Cuts at: 0 176 1063 2682 Size: 176 887 1619 Fragments arranged by size: 1619 887 176 MslI CAynn'nnrTG Cuts at: 0 1025 2065 2682 Size: 1025 1040 617 Fragments arranged by size: 1040 1025 617 MspA1I CmG'CkG Cuts at: 0 447 454 783 1151 1224 1374 2025 2303 Size: 447 7 329 368 73 150 651 278 Cuts at: 2303 2315 2564 2682 Size: 12 249 118 Fragments arranged by size: 651 447 368 329 278 249 150 118 73 12 7 NaeI GCC'GGC Cuts at: 0 761 1443 1455 1651 2682 Size: 761 682 12 196 1031 Fragments arranged by size: 1031 761 682 196 12 NarI GG'CG_CC Cuts at: 0 1862 2682 Size: 1862 820 NcoI C'CATG_G Cuts at: 0 1058 1079 2682 Size: 1058 21 1603 Fragments arranged by size: 1603 1058 21 NgoMIV G'CCGG_C Cuts at: 0 759 1441 1453 1649 2682 Size: 759 682 12 196 1033 Fragments arranged by size: 1033 759 682 196 12 NspI r_CATG'y Cuts at: 0 677 1434 2682 Size: 677 757 1248 Fragments arranged by size: 1248 757 677 PasI CC'CwG_GG Cuts at: 0 519 2682 Size: 519 2163 PflMI CCAn_nnn'nTGG Cuts at: 0 972 2682 Size: 972 1710 PmlI CAC'GTG Cuts at: 0 2390 2682 Size: 2390 292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2107 2139 2682 Size: 2107 32 543 Fragments arranged by size: 2107 543 32 PpuMI rG'GwC_Cy Cuts at: 0 1725 2076 2169 2271 2682 Size: 1725 351 93 102 411 Fragments arranged by size: 1725 411 351 102 93 PshAI GACnn'nnGTC Cuts at: 0 101 2682 Size: 101 2581 PspOMI G'GGCC_C Cuts at: 0 885 2346 2682 Size: 885 1461 336 Fragments arranged by size: 1461 885 336 PstI C_TGCA'G Cuts at: 0 559 713 2425 2682 Size: 559 154 1712 257 Fragments arranged by size: 1712 559 257 154 PvuII CAG'CTG Cuts at: 0 447 1374 2682 Size: 447 927 1308 Fragments arranged by size: 1308 927 447 SacI G_AGCT'C Cuts at: 0 50 317 1838 2682 Size: 50 267 1521 844 Fragments arranged by size: 1521 844 267 50 SacII CC_GC'GG Cuts at: 0 784 1152 2682 Size: 784 368 1530 Fragments arranged by size: 1530 784 368 SanDI GG'GwC_CC Cuts at: 0 1725 2682 Size: 1725 957 SapI GCTCTTCn'nnn_ Cuts at: 0 2008 2682 Size: 2008 674 ScaI AGT'ACT Cuts at: 0 2398 2682 Size: 2398 284 SfcI C'TryA_G Cuts at: 0 555 709 2421 2595 2682 Size: 555 154 1712 174 87 Fragments arranged by size: 1712 555 174 154 87 SfiI GGCCn_nnn'nGGCC Cuts at: 0 435 2294 2682 Size: 435 1859 388 Fragments arranged by size: 1859 435 388 SfoI GGC'GCC Cuts at: 0 1863 2682 Size: 1863 819 SgrAI Cr'CCGG_yG Cuts at: 0 1334 2441 2455 2682 Size: 1334 1107 14 227 Fragments arranged by size: 1334 1107 227 14 SmaI CCC'GGG Cuts at: 0 756 908 1145 1722 1730 1998 2037 2268 Size: 756 152 237 577 8 268 39 231 Cuts at: 2268 2345 2352 2682 Size: 77 7 330 Fragments arranged by size: 756 577 330 268 237 231 152 77 39 8 7 SmlI C'TyrA_G Cuts at: 0 1408 1528 2682 Size: 1408 120 1154 Fragments arranged by size: 1408 1154 120 SrfI GCCC'GGGC Cuts at: 0 756 2345 2682 Size: 756 1589 337 Fragments arranged by size: 1589 756 337 StuI AGG'CCT Cuts at: 0 438 2682 Size: 438 2244 StyI C'CwwG_G Cuts at: 0 197 1058 1079 1751 1809 2366 2682 Size: 197 861 21 672 58 557 316 Fragments arranged by size: 861 672 557 316 197 58 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 133 244 402 1052 2682 Size: 133 111 158 650 1630 Fragments arranged by size: 1630 650 158 133 111 TatI w'GTAC_w Cuts at: 0 2120 2396 2682 Size: 2120 276 286 Fragments arranged by size: 2120 286 276 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 225 257 576 608 2682 Size: 225 32 319 32 2074 Fragments arranged by size: 2074 319 225 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1039 2682 Size: 1039 1643 XmaI C'CCGG_G Cuts at: 0 754 906 1143 1720 1728 1996 2035 2266 Size: 754 152 237 577 8 268 39 231 Cuts at: 2266 2343 2350 2682 Size: 77 7 332 Fragments arranged by size: 754 577 332 268 237 231 152 77 39 8 7 XmnI GAAnn'nnTTC Cuts at: 0 1180 2682 Size: 1180 1502 ZraI GAC'GTC Cuts at: 0 2655 2682 Size: 2655 27 Enzymes that do cut: AarI AatII AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI SapI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI SrfI StuI StyI TaqII TatI TstI Tth111I XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI BaeI BbvCI BciVI BfrBI BglII BmtI BplI BsaWI BsiWI BsmI BsmBI BspEI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FspAI HindIII HpaI KpnI MfeI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PsiI PspXI PsrI PvuI RsrII SalI SbfI SexAI SgfI SnaBI SpeI SphI SspI SwaI XbaI XcmI XhoI