################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 910 GTAGAC GT^[AC][GT]AC AciI 1103 CCGC CCGC(-3/-1) AcsI 504 GAATTT [GA]^AATT[CT] AflIII 914 ACGTGT A^C[GA][CT]GT Alw44I 1057 GTGCAC G^TGCAC AlwI 655 GATCC GGATC(4/5) ApaLI 1057 GTGCAC G^TGCAC ApoI 504 GAATTT [GA]^AATT[CT] Asp700I 210 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 20 TGGCCA TGG^CCA BcnI 592 CCCGG CC^[GC]GG BfaI 13 CTAG C^TAG BglII 330 AGATCT A^GATCT BlpI 1066 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1066 GCTGAGC GC^T[ACGT]AGC BsaWI 1111 ACCGGA [AT]^CCGG[AT] BseRI 40 CTCCTC GAGGAG(10/8) BspHI 336 TCATGA T^CATGA BspMI 861 GCAGGT ACCTGC(4/8) BssSI 361 CACGAG CACGAG(-5/-1) BstXI 23 CCATTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 330 AGATCT [GA]^GATC[CT] CelII 1066 GCTGAGC GC^T[ACGT]AGC DraII 383 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 20 TGGCCA [CT]^GGCC[GA] EarI 1207 CTCTTC CTCTTC(1/4) EcoO109I 383 AGGACCT [GA]G^G[ACGT]CC[CT] HincII 1225 GTTAAC GT[CT]^[GA]AC HindII 1225 GTTAAC GT[CT]^[GA]AC HindIII 92 AAGCTT A^AGCTT HpaI 1225 GTTAAC GTT^AAC Ksp632I 1207 CTCTTC CTCTTC(1/4) MaeI 13 CTAG C^TAG MaeII 914 ACGT A^CGT MflI 330 AGATCT [GA]^GATC[CT] MluNI 20 TGGCCA TGG^CCA MscI 20 TGGCCA TGG^CCA MslI 597 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1103 CCGCTG C[AC]G^C[GT]G NciI 592 CCCGG CC^[GC]GG PpuMI 383 AGGACCT [GA]G^G[AT]CC[CT] PstI 430 CTGCAG CTGCA^G RcaI 336 TCATGA T^CATGA SapI 1206 GCTCTTC GCTCTTC(1/4) StuI 15 AGGCCT AGG^CCT Tsp45I 897 GTCAC ^GT[GC]AC XhoII 330 AGATCT [GA]^GATC[CT] XmnI 210 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 922 GTAC GT^AC 1091 GTAC AlwNI 424 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1120 CAGACTCTG AvaII 105 GGTCC G^G[AT]CC 384 GGACC BpmI 876 CTCCAG CTGGAG(16/14) 688 CTGGAG BsmAI 1135 GAGAC GTCTC(1/5) 982 GTCTC DsaI 555 CCATGG C^C[GA][CT]GG 1130 CCATGG HapII 593 CCGG C^CGG 1112 CCGG HpaII 593 CCGG C^CGG 1112 CCGG MaeIII 664 GTAAC ^GT[ACGT]AC 897 GTCAC MspI 593 CCGG C^CGG 1112 CCGG NcoI 555 CCATGG C^CATGG 1130 CCATGG RsaI 922 GTAC GT^AC 1091 GTAC SfcI 239 CTACAG C^T[GA][CT]AG 430 CTGCAG SmlI 178 CTCAAG C^T[CT][GA]AG 228 CTTGAG TspRI 474 CAGTG CA[GC]TG[ACGT][ACGT]^ 597 CACTG XcmI 8 CCACACTAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 251 CCATGCTGTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AluI AspHI BanI BbsI BbvI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp509I TthHB8I