(Circular) (Six-base) MAPSORT of: pFN21AE4391.seq Check: 1613 from: 1 to: 5947 pFN21AE4391 5947 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2857 2857 Size: 5947 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2916 3290 1937 Size: 979 374 4594 Fragments arranged by size: 4594 979 374 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2299 2864 2981 3098 4010 5394 5662 1632 Size: 667 565 117 117 912 1384 268 1917 Fragments arranged by size: 1917 1384 912 667 565 268 117 117 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 2919 4846 5823 1203 Size: 1716 1927 977 1327 Fragments arranged by size: 1927 1716 1327 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5947 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2624 1184 Size: 1440 4507 AlwNI CAG_nnn'CTG Cuts at: 2434 3130 3690 5262 2434 Size: 696 560 1572 3119 Fragments arranged by size: 3119 1572 696 560 ApaLI G'TGCA_C Cuts at: 3062 4004 5160 3062 Size: 942 1156 3849 Fragments arranged by size: 3849 1156 942 ApoI r'AATT_y Cuts at: 1493 2509 3256 3482 3634 5743 1493 Size: 1016 747 226 152 2109 1697 Fragments arranged by size: 2109 1697 1016 747 226 152 AseI AT'TA_AT Cuts at: 160 1032 4501 160 Size: 872 3469 1606 Fragments arranged by size: 3469 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 3272 1870 Size: 73 1329 4545 Fragments arranged by size: 4545 1329 73 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2270 2716 2948 3113 3268 Size: 325 217 627 483 446 232 165 155 Cuts at: 3268 4723 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 483 446 325 232 217 165 155 BanII G_rGCy'C Cuts at: 1391 3266 1391 Size: 1875 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2054 2387 2972 928 Size: 971 155 333 585 3903 Fragments arranged by size: 3903 971 585 333 155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4138 4172 1903 Size: 34 2201 34 3678 Fragments arranged by size: 3678 2201 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3839 5049 3839 Size: 1210 4737 BclI T'GATC_A Cuts at: 1548 1548 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4558 136 Size: 107 122 71 631 605 2886 1525 Fragments arranged by size: 2886 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2335 5942 2335 Size: 3607 2340 BlpI GC'TnA_GC Cuts at: 3072 3364 3072 Size: 292 5655 Bme1580I G_kGCm'C Cuts at: 1790 2275 2721 2951 3066 4008 5164 1790 Size: 485 446 230 115 942 1156 2573 Fragments arranged by size: 2573 1156 942 485 446 230 115 BmgBI CAC'GTC Cuts at: 1318 2920 1318 Size: 1602 4345 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3678 4636 442 Size: 416 53 83 399 24 2261 958 1753 Fragments arranged by size: 2261 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2714 2866 4607 1239 Size: 1475 152 1741 2579 Fragments arranged by size: 2579 1741 1475 152 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2168 2254 4072 4937 5235 2168 Size: 86 1818 865 298 2880 Fragments arranged by size: 2880 1818 865 298 86 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4610 882 Size: 617 3111 2219 Fragments arranged by size: 3111 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3738 493 Size: 711 2534 2702 Fragments arranged by size: 2702 2534 711 BsaBI GATnn'nnATC Cuts at: 5941 5941 Size: 5947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3754 4136 4771 275 Size: 53 83 186 936 2221 382 635 1451 Fragments arranged by size: 2221 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3116 4380 4778 5052 5199 5610 3116 Size: 1264 398 274 147 411 3453 Fragments arranged by size: 3453 1264 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2864 2894 2919 2949 1178 Size: 30 165 30 1461 30 25 30 4176 Fragments arranged by size: 4176 1461 165 30 30 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 1186 Size: 850 5097 BseYI C'CCAG_C Cuts at: 5150 5646 5150 Size: 496 5451 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5374 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3397 4158 5186 1540 Size: 275 186 1396 761 1028 2301 Fragments arranged by size: 2301 1396 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3066 3124 3213 4008 4093 5164 3066 Size: 58 89 795 85 1071 3849 Fragments arranged by size: 3849 1071 795 89 85 58 BsmI GAATG_Cn' Cuts at: 3458 3551 3458 Size: 93 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2275 2721 2951 3066 3124 3213 3266 Size: 399 485 446 230 115 58 89 53 Cuts at: 3266 4008 4093 5164 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 485 446 399 230 115 89 85 58 53 BspEI T'CCGG_A Cuts at: 5610 5610 Size: 5947 BspHI T'CATG_A Cuts at: 2341 3836 5566 2341 Size: 1495 1730 2722 Fragments arranged by size: 2722 1730 1495 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2857 3302 844 Size: 2013 445 3489 Fragments arranged by size: 3489 2013 445 BsrBI CCG'CTC Cuts at: 1394 1823 3834 1394 Size: 429 2011 3507 Fragments arranged by size: 3507 2011 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4442 4616 65 Size: 1183 3194 174 1396 Fragments arranged by size: 3194 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4591 4778 1700 Size: 110 2781 187 2869 Fragments arranged by size: 2869 2781 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5947 BssSI C'ACGA_G Cuts at: 2366 4007 5019 2366 Size: 1641 1012 3294 Fragments arranged by size: 3294 1641 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5947 BstBI TT'CG_AA Cuts at: 4751 4751 Size: 5947 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2035 Size: 5947 BstYI r'GATC_y Cuts at: 1972 2335 3277 3644 4029 4046 5498 5584 5596 Size: 363 942 367 385 17 1452 86 12 Cuts at: 5596 5942 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 942 385 367 363 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2560 3135 513 Size: 552 1495 575 3325 Fragments arranged by size: 3325 1495 575 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3020 487 Size: 683 56 1794 3414 Fragments arranged by size: 3414 1794 683 56 BtsI GCAGTG_nn' Cuts at: 3463 4257 4277 3463 Size: 794 20 5133 Fragments arranged by size: 5133 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 3251 3251 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 3394 4283 Size: 54 1425 50 275 18 195 1369 889 Cuts at: 4283 5912 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1369 889 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3394 1537 Size: 275 1582 4090 Fragments arranged by size: 4090 1582 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3218 3877 1353 Size: 1865 659 3423 Fragments arranged by size: 3423 1865 659 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4534 4908 5054 387 Size: 1064 138 2945 374 146 1280 Fragments arranged by size: 2945 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2299 2714 2864 2866 2981 3098 4010 Size: 393 667 415 150 2 115 117 912 Cuts at: 4010 4607 5394 5662 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 912 787 667 597 415 393 268 150 117 115 2 EcoO109I rG'GnC_Cy Cuts at: 2389 2389 Size: 5947 EcoRI G'AATT_C Cuts at: 3256 3634 3256 Size: 378 5569 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2092 2212 2092 Size: 120 5827 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 5094 1678 Size: 3416 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2753 2785 2864 2896 3016 Size: 32 135 32 1181 32 79 32 120 Cuts at: 3016 3048 4588 4620 4662 4694 1373 Size: 32 1540 32 42 32 2626 Fragments arranged by size: 2626 1540 1181 135 120 79 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3232 3291 3537 669 Size: 988 281 1294 59 246 3079 Fragments arranged by size: 3079 1294 988 281 246 59 HindIII A'AGCT_T Cuts at: 748 2097 748 Size: 1349 4598 HpaI GTT'AAC Cuts at: 3232 3537 3232 Size: 305 5642 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 3546 3546 Size: 5947 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5061 5245 1305 Size: 3756 184 2007 Fragments arranged by size: 3756 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 10 Size: 54 1425 343 195 3930 Fragments arranged by size: 3930 1425 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2606 3905 4264 4423 518 Size: 648 1440 1299 359 159 2042 Fragments arranged by size: 2042 1440 1299 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3110 3363 4040 5188 5433 1606 Size: 1504 253 677 1148 245 2120 Fragments arranged by size: 2120 1504 1148 677 253 245 NcoI C'CATG_G Cuts at: 513 1065 2560 3135 513 Size: 552 1495 575 3325 Fragments arranged by size: 3325 1495 575 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5947 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 3305 4850 1132 Size: 97 2076 1545 2229 Fragments arranged by size: 2229 2076 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5947 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5947 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 5947 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2389 2389 Size: 5947 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5947 PsiI TTA'TAA Cuts at: 2094 3517 2094 Size: 1423 4524 PstI C_TGCA'G Cuts at: 830 2439 3299 830 Size: 1609 860 3478 Fragments arranged by size: 3478 1609 860 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SapI GCTCTTCn'nnn_ Cuts at: 3218 3218 Size: 5947 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 4195 5907 4195 Size: 1712 4235 SfcI C'TryA_G Cuts at: 826 1045 2244 2435 3295 4430 5111 5302 826 Size: 219 1199 191 860 1135 681 191 1471 Fragments arranged by size: 1471 1199 1135 860 681 219 191 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 3274 1872 Size: 1402 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 2233 4051 4952 5214 820 Size: 197 926 240 50 1818 901 262 1553 Fragments arranged by size: 1818 1553 926 901 262 240 197 50 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 3305 1132 Size: 2173 3774 SspI AAT'ATT Cuts at: 5 52 3871 5 Size: 47 3819 2081 Fragments arranged by size: 3819 2081 47 StuI AGG'CCT Cuts at: 1517 2022 1517 Size: 505 5442 StyI C'CwwG_G Cuts at: 513 1065 2084 2113 2139 2560 3135 3386 513 Size: 552 1019 29 26 421 575 251 3074 Fragments arranged by size: 3074 1019 575 552 421 251 29 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 3974 3991 4144 4329 4774 325 Size: 1255 678 1716 17 153 185 445 1498 Fragments arranged by size: 1716 1498 1255 678 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4193 5905 96 Size: 274 80 33 51 1443 2216 1712 138 Fragments arranged by size: 2216 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2853 2885 5907 5939 2853 Size: 32 3022 32 2861 Fragments arranged by size: 3022 2861 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2020 2263 2020 Size: 243 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 3272 1870 Size: 1402 4545 XmnI GAAnn'nnTTC Cuts at: 2219 4076 2219 Size: 1857 4090 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI