################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI AluI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbsI BbvCI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MroI MseI MslI MspA1I MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 229 CGCG CG^CG AocI 625 CCTGAGG CC^T[ACGT]AGG AspHI 274 GAGCAC G[AT]GC[AT]^C BalI 512 TGGCCA TGG^CCA BanII 505 GAGCCC G[GA]GC[CT]^C BbrPI 616 CACGTG CAC^GTG BcgI 369 CGACCAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 597 GGATAC GTATCC(6/5) BglII 209 AGATCT A^GATCT BpmI 181 CTGGAG CTGGAG(16/14) BsaBI 471 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 83 ACCGGA [AT]^CCGG[AT] BsiHKAI 274 GAGCAC G[AT]GC[AT]^C BspMI 650 ACCTGC ACCTGC(4/8) BsrDI 564 CATTGC GCAATG(2/0) BsrI 321 ACTGG ACTGG(1/-1) BssKI 180 CCTGG ^CC[ACGT]GG BstAPI 396 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 180 CCTGG CC^[AT]GG BstUI 229 CGCG CG^CG BstYI 209 AGATCT [GA]^GATC[CT] Bsu36I 625 CCTGAGG CC^T[ACGT]AGG CfoI 405 GCGC GCG^C EaeI 512 TGGCCA [CT]^GGCC[GA] EarI 198 GAAGAG CTCTTC(1/4) Eco81I 625 CCTGAGG CC^T[ACGT]AGG EcoRII 180 CCTGG ^CC[AT]GG HhaI 405 GCGC GCG^C HinP1I 405 GCGC G^CGC HinfI 184 GAGTC G^A[ACGT]TC HphI 116 TCACC GGTGA(8/7) Ksp632I 198 GAAGAG CTCTTC(1/4) MamI 471 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 209 AGATCT [GA]^GATC[CT] MluI 228 ACGCGT A^CGCGT MluNI 512 TGGCCA TGG^CCA MscI 512 TGGCCA TGG^CCA MvaI 180 CCTGG CC^[AT]GG MvnI 229 CGCG CG^CG MwoI 396 GCAGTTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 509 CCATGG C^CATGG NlaIII 510 CATG CATG^ PleI 184 GAGTC GAGTC(4/5) PmaCI 616 CACGTG CAC^GTG PmlI 616 CACGTG CAC^GTG PstI 235 CTGCAG CTGCA^G SapI 198 GAAGAGC GCTCTTC(1/4) ScrFI 180 CCTGG CC^[ACGT]GG TaqI 95 TCGA T^CGA TthHB8I 95 TCGA T^CGA XhoII 209 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AfaI 232 GTAC GT^AC 582 GTAC AflIII 228 ACGCGT A^C[GA][CT]GT 617 ACGTGT AlwNI 397 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 553 CAGGCCCTG Asp700I 147 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 357 GAAGGTGTTC AvaII 60 GGACC G^G[AT]CC 384 GGACC BbvI 688 GCAGC GCAGC(8/12) 259 GCTGC BmyI 274 GAGCAC G[AGT]GC[ACT]^C 505 GAGCCC BsaAI 583 TACGTG [CT]AC^GT[GA] 616 CACGTG BseRI 271 GAGGAG GAGGAG(10/8) 466 GAGGAG BsgI 489 CTGCAC GTGCAG(16/14) 394 GTGCAG BsmFI 59 GGGAC GGGAC(10/14) 186 GTCCC Bsp1286I 274 GAGCAC G[AGT]GC[ACT]^C 505 GAGCCC DpnII 210 GATC ^GATC 537 GATC DraII 160 AGGGCCT [GA]G^G[ACGT]CC[CT] 554 AGGCCCT DsaI 509 CCATGG C^C[GA][CT]GG 515 CCACGG EcoNI 351 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 422 CCTTCATCAGG EcoO109I 160 AGGGCCT [GA]G^G[ACGT]CC[CT] 554 AGGCCCT EcoT14I 164 CCTTGG C^C[AT][AT]GG 509 CCATGG HapII 84 CCGG C^CGG 450 CCGG HpaII 84 CCGG C^CGG 450 CCGG MaeIII 38 GTGAC ^GT[ACGT]AC 524 GTGAC MboI 210 GATC ^GATC 537 GATC MspI 84 CCGG C^CGG 450 CCGG NdeII 210 GATC ^GATC 537 GATC NlaIV 59 GGGACC GG[ACGT]^[ACGT]CC 431 GGAACC RsaI 232 GTAC GT^AC 582 GTAC Sau3AI 210 GATC ^GATC 537 GATC SfcI 156 CTACAG C^T[GA][CT]AG 235 CTGCAG StyI 164 CCTTGG C^C[AT][AT]GG 509 CCATGG TseI 259 GCTGC G^C[AT]GC 688 GCAGC Tsp45I 38 GTGAC ^GT[GC]AC 524 GTGAC TspRI 309 CAGTG CA[GC]TG[ACGT][ACGT]^ 341 CAGTG XmnI 147 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 357 GAAGGTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AciI BsaJI BsiYI BslI BstF5I Cac8I Cfr13I DdeI DraIII Fnu4HI FokI HaeIII ItaI MaeII MboII MnlI Sau96I