################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## Acc65I AccII AccIII AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbrPI BbvCI BcgI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HgaI HindIII HpaI KpnI KspI MamI MluI MluNI MroI MscI MvnI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 64 GACGTC GACGT^C AccI 61 GTCGAC GT^[AC][GT]AC AclI 560 AACGTT AA^CGTT Asp700I 1219 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 135 GTGCTC G[AT]GC[AT]^C BbeI 16 GGCGCC GGCGC^C BciVI 1074 GGATAC GTATCC(6/5) BclI 657 TGATCA T^GATCA BcnI 744 CCGGG CC^[GC]GG BglII 972 AGATCT A^GATCT BlpI 877 GCTAAGC GC^T[ACGT]AGC Bpu1102I 877 GCTAAGC GC^T[ACGT]AGC BsaI 414 GAGACC GGTCTC(1/5) BsiHKAI 135 GTGCTC G[AT]GC[AT]^C BsmI 328 GAATGC GAATGC(1/-1) BsrFI 781 GCCGGC [GA]^CCGG[CT] BstAPI 169 GCACTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 972 AGATCT [GA]^GATC[CT] CelII 877 GCTAAGC GC^T[ACGT]AGC Cfr10I 781 GCCGGC [GA]^CCGG[CT] Cfr13I 914 GGCCC G^G[ACGT]CC DraI 316 TTTAAA TTT^AAA EaeI 2 TGGCCG [CT]^GGCC[GA] EcoT14I 486 CCATGG C^C[AT][AT]GG FbaI 657 TGATCA T^GATCA HaeII 16 GGCGCC [GA]GCGC^[CT] HincII 61 GTCGAC GT[CT]^[GA]AC HindII 61 GTCGAC GT[CT]^[GA]AC HphI 655 GGTGA GGTGA(8/7) KasI 16 GGCGCC G^GCGCC MfeI 592 CAATTG C^AATTG MflI 972 AGATCT [GA]^GATC[CT] MslI 496 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 20 CCGCTG C[AC]G^C[GT]G MunI 592 CAATTG C^AATTG NaeI 781 GCCGGC GCC^GGC NarI 16 GGCGCC GG^CGCC NciI 744 CCGGG CC^[GC]GG NcoI 486 CCATGG C^CATGG NgoMIV 781 GCCGGC G^CCGGC Psp1406I 560 AACGTT AA^CGTT PstI 1269 CTGCAG CTGCA^G SalI 61 GTCGAC G^TCGAC Sau96I 914 GGCCC G^G[ACGT]CC SfoI 16 GGCGCC GGC^GCC StuI 548 AGGCCT AGG^CCT StyI 486 CCATGG C^C[AT][AT]GG TaqI 62 TCGA T^CGA Tsp45I 254 GTCAC ^GT[GC]AC TthHB8I 62 TCGA T^CGA XbaI 975 TCTAGA T^CTAGA XhoII 972 AGATCT [GA]^GATC[CT] XmnI 1219 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 16 GGCGCC G[GA]^CG[CT]C 64 GACGTC BanI 16 GGCGCC G^G[CT][GA]CC 502 GGTGCC BanII 43 GAGCCC G[GA]GC[CT]^C 150 GGGCTC BfaI 788 CTAG C^TAG 976 CTAG BsaHI 16 GGCGCC G[GA]^CG[CT]C 64 GACGTC BseRI 73 GAGGAG GAGGAG(10/8) 82 GAGGAG BsgI 189 CTGCAC GTGCAG(16/14) 587 CTGCAC BsmAI 162 GAGAC GTCTC(1/5) 414 GAGAC BspMI 187 ACCTGC ACCTGC(4/8) 943 ACCTGC CfoI 17 GCGC GCG^C 104 GCGC DsaI 47 CCGTGG C^C[GA][CT]GG 486 CCATGG EarI 1219 GAAGAG CTCTTC(1/4) 1244 GAAGAG EcoNI 69 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 705 CCTCAAAGAGG HhaI 17 GCGC GCG^C 104 GCGC Hin1I 16 GGCGCC G[GA]^CG[CT]C 64 GACGTC HinP1I 17 GCGC G^CGC 104 GCGC Ksp632I 1219 GAAGAG CTCTTC(1/4) 1244 GAAGAG MaeI 788 CTAG C^TAG 976 CTAG MaeII 65 ACGT A^CGT 561 ACGT MaeIII 254 GTCAC ^GT[ACGT]AC 601 GTAAC PflMI 905 CCACCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1101 CCAGCAAGTGG PleI 124 GACTC GAGTC(4/5) 1097 GAGTC SfcI 180 CTGTAG C^T[GA][CT]AG 1269 CTGCAG SmlI 70 CTTGAG C^T[CT][GA]AG 1159 CTCAAG Van91I 905 CCACCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1101 CCAGCAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbsI BbvI BmyI BpmI BpuAI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI