(Circular) (Six-base) MAPSORT of: pFN21AE1143.seq Check: 8361 from: 1 to: 5980 pFN21AE1143 5980 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2073 3790 278 Size: 53 83 186 936 537 1717 2468 Fragments arranged by size: 2468 1717 936 537 186 83 53 AccI GT'mk_AC Cuts at: 1937 2067 3323 1937 Size: 130 1256 4594 Fragments arranged by size: 4594 1256 130 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 2566 4107 4480 1563 Size: 1003 1541 373 3063 Fragments arranged by size: 3063 1541 1003 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2457 2852 4043 5427 5695 1632 Size: 825 395 1191 1384 268 1917 Fragments arranged by size: 1917 1384 1191 825 395 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2523 1184 Size: 1339 4641 AlwNI CAG_nnn'CTG Cuts at: 3723 5295 3723 Size: 1572 4408 ApaLI G'TGCA_C Cuts at: 4037 5193 4037 Size: 1156 4824 ApoI r'AATT_y Cuts at: 1493 2223 2290 2326 2365 3289 3515 3667 5776 Size: 730 67 36 39 924 226 152 2109 Cuts at: 5776 1493 Size: 1697 Fragments arranged by size: 2109 1697 924 730 226 152 67 39 36 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 3305 1870 Size: 73 1362 4545 Fragments arranged by size: 4545 1362 73 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2507 3301 4756 618 Size: 325 217 627 234 486 794 1455 1842 Fragments arranged by size: 1842 1455 794 627 486 325 234 217 BanII G_rGCy'C Cuts at: 1391 2052 2159 3299 1391 Size: 661 107 1140 4072 Fragments arranged by size: 4072 1140 661 107 BbeI G_GCGC'C Cuts at: 2025 2025 Size: 5980 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2445 2487 3149 928 Size: 971 546 42 662 3759 Fragments arranged by size: 3759 971 662 546 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4171 4205 1903 Size: 34 2234 34 3678 Fragments arranged by size: 3678 2234 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3073 3872 5082 3073 Size: 799 1210 3971 Fragments arranged by size: 3971 1210 799 BclI T'GATC_A Cuts at: 1548 2662 1548 Size: 1114 4866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4591 136 Size: 107 122 71 631 605 2919 1525 Fragments arranged by size: 2919 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2977 5975 2977 Size: 2998 2982 BlpI GC'TnA_GC Cuts at: 2883 3397 2883 Size: 514 5466 Bme1580I G_kGCm'C Cuts at: 1790 4041 5197 1790 Size: 2251 1156 2573 Fragments arranged by size: 2573 2251 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4669 442 Size: 416 53 83 399 24 2294 958 1753 Fragments arranged by size: 2294 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2158 2158 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2114 2795 4640 1239 Size: 827 48 681 1845 2579 Fragments arranged by size: 2579 1845 827 681 48 Bpu10I CC'TnA_GC Cuts at: 3086 3086 Size: 5980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2096 3149 4105 4970 5268 2096 Size: 1053 956 865 298 2808 Fragments arranged by size: 2808 1053 956 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2413 4643 882 Size: 617 914 2230 2219 Fragments arranged by size: 2230 2219 914 617 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 2070 3787 4169 Size: 53 83 186 936 489 48 1717 382 Cuts at: 4169 4804 275 Size: 635 1451 Fragments arranged by size: 1717 1451 936 635 489 382 186 83 53 48 BsaWI w'CCGG_w Cuts at: 4413 4811 5085 5232 5643 4413 Size: 398 274 147 411 4750 Fragments arranged by size: 4750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5755 Fragments arranged by size: 5755 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2102 1186 Size: 907 9 5064 Fragments arranged by size: 5064 907 9 BseYI C'CCAG_C Cuts at: 2027 5183 5679 2027 Size: 3156 496 2328 Fragments arranged by size: 3156 2328 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2179 2577 1163 Size: 573 443 398 4566 Fragments arranged by size: 4566 573 443 398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2144 4041 4126 5197 2144 Size: 1897 85 1071 2927 Fragments arranged by size: 2927 1897 1071 85 BsmI GAATG_Cn' Cuts at: 2339 3491 3584 2339 Size: 1152 93 4735 Fragments arranged by size: 4735 1152 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2144 2159 3299 4041 4126 5197 Size: 399 262 92 15 1140 742 85 1071 Cuts at: 5197 1391 Size: 2174 Fragments arranged by size: 2174 1140 1071 742 399 262 92 85 15 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2201 2957 3335 844 Size: 1357 756 378 3489 Fragments arranged by size: 3489 1357 756 378 BsrBI CCG'CTC Cuts at: 1394 1823 3867 1394 Size: 429 2044 3507 Fragments arranged by size: 3507 2044 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2786 4624 4811 1700 Size: 110 976 1838 187 2869 Fragments arranged by size: 2869 1838 976 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 2180 794 Size: 1386 4594 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstYI r'GATC_y Cuts at: 1972 2977 3310 3677 4062 4079 5531 5617 5629 Size: 1005 333 367 385 17 1452 86 12 Cuts at: 5629 5975 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1005 385 367 346 333 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 2491 513 Size: 552 939 48 439 4002 Fragments arranged by size: 4002 939 552 439 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5241 Fragments arranged by size: 5241 683 56 BtsI GCAGTG_nn' Cuts at: 2904 3496 4290 4310 2904 Size: 592 794 20 4574 Fragments arranged by size: 4574 794 592 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 2323 3284 2323 Size: 961 5019 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3427 4316 Size: 54 1425 50 275 18 177 1420 889 Cuts at: 4316 5945 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1420 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3219 3244 3910 1353 Size: 1866 25 666 3423 Fragments arranged by size: 3423 1866 666 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4567 4941 5087 387 Size: 1064 138 2978 374 146 1280 Fragments arranged by size: 2978 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2114 2457 2795 2852 4043 4640 Size: 393 434 48 343 338 57 1191 597 Cuts at: 4640 5427 5695 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1191 787 597 434 393 343 338 268 57 48 EcoNI CCTnn'n_nnAGG Cuts at: 2078 2714 2078 Size: 636 5344 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 2025 5127 1678 Size: 347 3102 2531 Fragments arranged by size: 3102 2531 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2410 2442 3218 3250 4621 Size: 32 135 32 838 32 776 32 1371 Cuts at: 4621 4653 4695 4727 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1371 838 776 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2068 3324 3570 669 Size: 988 281 130 1256 246 3079 Fragments arranged by size: 3079 1256 988 281 246 130 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KasI G'GCGC_C Cuts at: 2021 2021 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 2597 3579 2597 Size: 982 4998 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2019 5094 5278 1305 Size: 714 3075 184 2007 Fragments arranged by size: 3075 2007 714 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4158 Fragments arranged by size: 4158 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2505 3938 4297 4456 518 Size: 648 1339 1433 359 159 2042 Fragments arranged by size: 2042 1433 1339 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 3396 4073 5221 5466 1606 Size: 421 1369 677 1148 245 2120 Fragments arranged by size: 2120 1369 1148 677 421 245 NaeI GCC'GGC Cuts at: 2788 2788 Size: 5980 NarI GG'CG_CC Cuts at: 2022 2022 Size: 5980 NcoI C'CATG_G Cuts at: 513 1065 2004 2491 513 Size: 552 939 487 4002 Fragments arranged by size: 4002 939 552 487 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NgoMIV G'CCGG_C Cuts at: 2786 2786 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 3338 4883 1132 Size: 97 2109 1545 2229 Fragments arranged by size: 2229 2109 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5980 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 2916 3112 1108 Size: 1808 196 3976 Fragments arranged by size: 3976 1808 196 PfoI T'CCnGG_A Cuts at: 2748 2748 Size: 5980 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 2894 3550 2894 Size: 656 5324 PstI C_TGCA'G Cuts at: 830 3278 3332 830 Size: 2448 54 3478 Fragments arranged by size: 3478 2448 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 SalI G'TCGA_C Cuts at: 1936 2066 3322 1936 Size: 130 1256 4594 Fragments arranged by size: 4594 1256 130 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 2185 3274 3328 4463 5144 5335 826 Size: 219 1140 1089 54 1135 681 191 1471 Fragments arranged by size: 1471 1140 1135 1089 681 219 191 54 SfoI GGC'GCC Cuts at: 2023 2023 Size: 5980 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 3307 1872 Size: 1435 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2075 3164 4084 4985 5247 820 Size: 197 926 132 1089 920 901 262 1553 Fragments arranged by size: 1553 1089 926 920 901 262 197 132 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 2555 1517 Size: 1038 4942 StyI C'CwwG_G Cuts at: 513 1065 2004 2491 3419 513 Size: 552 939 487 928 3074 Fragments arranged by size: 3074 939 928 552 487 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4007 4024 4177 4362 4807 325 Size: 1255 2427 17 153 185 445 1498 Fragments arranged by size: 2427 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 2403 4226 Size: 274 80 33 51 1443 402 24 1823 Cuts at: 4226 5938 96 Size: 1712 138 Fragments arranged by size: 1823 1712 1443 402 274 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5940 5972 5940 Size: 32 5948 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 2980 3316 2980 Size: 336 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 3305 1870 Size: 1435 4545 XmnI GAAnn'nnTTC Cuts at: 3228 4109 3228 Size: 881 5099 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2071 3788 276 Size: 53 83 186 936 537 1717 2468 Fragments arranged by size: 2468 1717 936 537 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI