(Linear) (Six-base) MAPSORT of: KIEE1143.seq Check: 6542 from: 1 to: 1275 KIEE1143.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AatII G_ACGT'C Cuts at: 0 68 1275 Size: 68 1207 AccI GT'mk_AC Cuts at: 0 62 1275 Size: 62 1213 AclI AA'CG_TT Cuts at: 0 561 1275 Size: 561 714 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 452 847 1275 Size: 452 395 428 Fragments arranged by size: 452 428 395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 518 1275 Size: 518 757 ApoI r'AATT_y Cuts at: 0 218 285 321 360 1275 Size: 218 67 36 39 915 Fragments arranged by size: 915 218 67 39 36 BanI G'GyrC_C Cuts at: 0 16 502 1275 Size: 16 486 773 Fragments arranged by size: 773 486 16 BanII G_rGCy'C Cuts at: 0 47 154 1275 Size: 47 107 1121 Fragments arranged by size: 1121 107 47 BbeI G_GCGC'C Cuts at: 0 20 1275 Size: 20 1255 BbsI GAAGACnn'nnnn_ Cuts at: 0 440 482 1144 1275 Size: 440 42 662 131 Fragments arranged by size: 662 440 131 42 BciVI GTATCCnnnnn_n' Cuts at: 0 1068 1275 Size: 1068 207 BclI T'GATC_A Cuts at: 0 657 1275 Size: 657 618 BglII A'GATC_T Cuts at: 0 972 1275 Size: 972 303 BlpI GC'TnA_GC Cuts at: 0 878 1275 Size: 878 397 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 153 1275 Size: 153 1122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 109 790 1275 Size: 61 48 681 485 Fragments arranged by size: 681 485 61 48 Bpu10I CC'TnA_GC Cuts at: 0 1081 1275 Size: 1081 194 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 1144 1275 Size: 91 1053 131 Fragments arranged by size: 1053 131 91 BsaI GGTCTCn'nnnn_ Cuts at: 0 408 1275 Size: 408 867 BsaHI Gr'CG_yC Cuts at: 0 17 65 1275 Size: 17 48 1210 Fragments arranged by size: 1210 48 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 88 97 1275 Size: 88 9 1178 Fragments arranged by size: 1178 88 9 BseYI C'CCAG_C Cuts at: 0 22 1275 Size: 22 1253 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 174 572 1275 Size: 174 398 703 Fragments arranged by size: 703 398 174 BsiHKAI G_wGCw'C Cuts at: 0 139 1275 Size: 139 1136 BsmI GAATG_Cn' Cuts at: 0 334 1275 Size: 334 941 Bsp1286I G_dGCh'C Cuts at: 0 47 139 154 1275 Size: 47 92 15 1121 Fragments arranged by size: 1121 92 47 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 196 952 1275 Size: 196 756 323 Fragments arranged by size: 756 323 196 BsrFI r'CCGG_y Cuts at: 0 781 1275 Size: 781 494 BstAPI GCAn_nnn'nTGC Cuts at: 0 175 1275 Size: 175 1100 BstYI r'GATC_y Cuts at: 0 972 1275 Size: 972 303 BtgI C'CryG_G Cuts at: 0 47 486 1275 Size: 47 439 789 Fragments arranged by size: 789 439 47 BtsI GCAGTG_nn' Cuts at: 0 899 1275 Size: 899 376 DraI TTT'AAA Cuts at: 0 318 1275 Size: 318 957 EaeI y'GGCC_r Cuts at: 0 2 1275 Size: 2 1273 EarI CTCTTCn'nnn_ Cuts at: 0 1214 1239 1275 Size: 1214 25 36 Fragments arranged by size: 1214 36 25 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 109 452 790 847 1275 Size: 61 48 343 338 57 428 Fragments arranged by size: 428 343 338 61 57 48 EcoNI CCTnn'n_nnAGG Cuts at: 0 73 709 1275 Size: 73 636 566 Fragments arranged by size: 636 566 73 HaeII r_GCGC'y Cuts at: 0 20 1275 Size: 20 1255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 405 437 1213 1245 1275 Size: 405 32 776 32 30 Fragments arranged by size: 776 405 32 32 30 HincII GTy'rAC Cuts at: 0 63 1275 Size: 63 1212 KasI G'GCGC_C Cuts at: 0 16 1275 Size: 16 1259 MfeI C'AATT_G Cuts at: 0 592 1275 Size: 592 683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 14 1275 Size: 14 1261 MslI CAynn'nnrTG Cuts at: 0 500 1275 Size: 500 775 MspA1I CmG'CkG Cuts at: 0 22 1275 Size: 22 1253 NaeI GCC'GGC Cuts at: 0 783 1275 Size: 783 492 NarI GG'CG_CC Cuts at: 0 17 1275 Size: 17 1258 NcoI C'CATG_G Cuts at: 0 486 1275 Size: 486 789 NgoMIV G'CCGG_C Cuts at: 0 781 1275 Size: 781 494 PflMI CCAn_nnn'nTGG Cuts at: 0 911 1107 1275 Size: 911 196 168 Fragments arranged by size: 911 196 168 PfoI T'CCnGG_A Cuts at: 0 743 1275 Size: 743 532 PsiI TTA'TAA Cuts at: 0 889 1275 Size: 889 386 PstI C_TGCA'G Cuts at: 0 1273 1275 Size: 1273 2 SalI G'TCGA_C Cuts at: 0 61 1275 Size: 61 1214 SfcI C'TryA_G Cuts at: 0 180 1269 1275 Size: 180 1089 6 Fragments arranged by size: 1089 180 6 SfoI GGC'GCC Cuts at: 0 18 1275 Size: 18 1257 SmlI C'TyrA_G Cuts at: 0 70 1159 1275 Size: 70 1089 116 Fragments arranged by size: 1089 116 70 StuI AGG'CCT Cuts at: 0 550 1275 Size: 550 725 StyI C'CwwG_G Cuts at: 0 486 1275 Size: 486 789 TatI w'GTAC_w Cuts at: 0 374 398 1275 Size: 374 24 877 Fragments arranged by size: 877 374 24 XbaI T'CTAG_A Cuts at: 0 975 1275 Size: 975 300 XmnI GAAnn'nnTTC Cuts at: 0 1223 1275 Size: 1223 52 ZraI GAC'GTC Cuts at: 0 66 1275 Size: 66 1209 Enzymes that do cut: AatII AccI AclI AcuI AlfI ApoI BanI BanII BbeI BbsI BciVI BclI BglII BlpI BplI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BstAPI BstYI BtgI BtsI DraI EaeI EarI Eco57MI EcoNI HaeII Hin4I HincII KasI MfeI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PflMI PfoI PsiI PstI SalI SfcI SfoI SmlI StuI StyI TatI XbaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BfrBI BglI Bme1580I BmgBI BmrI BmtI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MluI MscI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XcmI XhoI XmaI