################################## # NO CUT ENZYMES ( 199 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I Cac8I CelII ClaI CpoI DpnII DraI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FokI FseI FspI HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 17 CGCG CG^CG AcsI 74 GAATTC [GA]^AATT[CT] AcyI 90 GGCGTC G[GA]^CG[CT]C AluI 101 AGCT AG^CT ApoI 74 GAATTC [GA]^AATT[CT] Asp700I 211 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 264 GGTCC G^G[AT]CC BbvCI 8 CCTCAGC CCTCAGC(-5/-2) BfaI 278 CTAG C^TAG BsaHI 90 GGCGTC G[GA]^CG[CT]C BsaJI 79 CCTTGG C^C[ACGT][ACGT]GG BsmAI 163 GAGAC GTCTC(1/5) BsmI 222 GAATGC GAATGC(1/-1) BsrFI 54 GCCGGT [GA]^CCGG[CT] BsrI 166 ACTGG ACTGG(1/-1) BssKI 267 CCAGG ^CC[ACGT]GG BstNI 267 CCAGG CC^[AT]GG BstUI 17 CGCG CG^CG Cfr10I 54 GCCGGT [GA]^CCGG[CT] Cfr13I 264 GGTCC G^G[ACGT]CC DdeI 9 CTCAG C^T[ACGT]AG DrdI 84 GACTGCGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 126 CGGCCA [CT]^GGCC[GA] EcoRI 74 GAATTC G^AATTC EcoRII 267 CCAGG ^CC[AT]GG EcoT14I 79 CCTTGG C^C[AT][AT]GG HaeII 30 GGCGCT [GA]GCGC^[CT] HapII 55 CCGG C^CGG HgaI 91 GCGTC GACGC(5/10) Hin1I 90 GGCGTC G[GA]^CG[CT]C HinfI 96 GAGTC G^A[ACGT]TC HpaII 55 CCGG C^CGG MaeI 278 CTAG C^TAG MspA1I 100 CAGCTG C[AC]G^C[GT]G MspI 55 CCGG C^CGG MvaI 267 CCAGG CC^[AT]GG MvnI 17 CGCG CG^CG NlaIII 130 CATG CATG^ PleI 96 GAGTC GAGTC(4/5) PvuII 100 CAGCTG CAG^CTG Sau96I 264 GGTCC G^G[ACGT]CC ScrFI 267 CCAGG CC^[ACGT]GG StyI 79 CCTTGG C^C[AT][AT]GG TaqI 94 TCGA T^CGA Tsp509I 75 AATT ^AATT TthHB8I 94 TCGA T^CGA XmnI 211 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 3 enzymes) # ################################### CfoI 16 GCGC GCG^C 31 GCGC HhaI 16 GCGC GCG^C 31 GCGC HinP1I 16 GCGC G^CGC 31 GCGC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AciI BbvI Fnu4HI HaeIII ItaI MboII MnlI MwoI TseI