(Linear) (Six-base) MAPSORT of: KIEE6519.seq Check: 2302 from: 1 to: 639 KIEE6519.orf With 182 enzymes: SgfI * March 16, 2015 11:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 181 354 639 Size: 181 173 285 Fragments arranged by size: 285 181 173 Acc65I G'GTAC_C Cuts at: 0 217 639 Size: 217 422 AleI CACnn'nnGTG Cuts at: 0 490 559 639 Size: 490 69 80 Fragments arranged by size: 490 80 69 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 535 567 639 Size: 535 32 72 Fragments arranged by size: 535 72 32 AlwNI CAG_nnn'CTG Cuts at: 0 456 639 Size: 456 183 ApoI r'AATT_y Cuts at: 0 120 279 639 Size: 120 159 360 Fragments arranged by size: 360 159 120 BanI G'GyrC_C Cuts at: 0 217 639 Size: 217 422 BanII G_rGCy'C Cuts at: 0 78 639 Size: 78 561 BbsI GAAGACnn'nnnn_ Cuts at: 0 356 639 Size: 356 283 BmrI ACTGGGnnnn_n' Cuts at: 0 549 639 Size: 549 90 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 168 582 639 Size: 168 414 57 Fragments arranged by size: 414 168 57 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 161 352 639 Size: 36 125 191 287 Fragments arranged by size: 287 191 125 36 BsaWI w'CCGG_w Cuts at: 0 324 639 Size: 324 315 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 40 472 639 Size: 40 432 167 Fragments arranged by size: 432 167 40 BseYI C'CCAG_C Cuts at: 0 185 379 533 639 Size: 185 194 154 106 Fragments arranged by size: 194 185 154 106 BsiHKAI G_wGCw'C Cuts at: 0 78 497 509 639 Size: 78 419 12 130 Fragments arranged by size: 419 130 78 12 Bsp1286I G_dGCh'C Cuts at: 0 78 497 509 639 Size: 78 419 12 130 Fragments arranged by size: 419 130 78 12 BspEI T'CCGG_A Cuts at: 0 324 639 Size: 324 315 BspHI T'CATG_A Cuts at: 0 431 639 Size: 431 208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 181 354 639 Size: 181 173 285 Fragments arranged by size: 285 181 173 BstBI TT'CG_AA Cuts at: 0 211 639 Size: 211 428 BstYI r'GATC_y Cuts at: 0 243 327 639 Size: 243 84 312 Fragments arranged by size: 312 243 84 BtsI GCAGTG_nn' Cuts at: 0 592 639 Size: 592 47 DraIII CAC_nnn'GTG Cuts at: 0 584 639 Size: 584 55 EarI CTCTTCn'nnn_ Cuts at: 0 296 639 Size: 296 343 EcoICRI GAG'CTC Cuts at: 0 76 639 Size: 76 563 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 161 352 639 Size: 36 125 191 287 Fragments arranged by size: 287 191 125 36 EcoO109I rG'GnC_Cy Cuts at: 0 573 588 612 639 Size: 573 15 24 27 Fragments arranged by size: 573 27 24 15 EcoRI G'AATT_C Cuts at: 0 120 639 Size: 120 519 KpnI G_GTAC'C Cuts at: 0 221 639 Size: 221 418 MslI CAynn'nnrTG Cuts at: 0 110 490 559 639 Size: 110 380 69 80 Fragments arranged by size: 380 110 80 69 MspA1I CmG'CkG Cuts at: 0 240 639 Size: 240 399 NspI r_CATG'y Cuts at: 0 257 639 Size: 257 382 PfoI T'CCnGG_A Cuts at: 0 515 639 Size: 515 124 PpuMI rG'GwC_Cy Cuts at: 0 573 588 612 639 Size: 573 15 24 27 Fragments arranged by size: 573 27 24 15 PstI C_TGCA'G Cuts at: 0 599 639 Size: 599 40 PvuII CAG'CTG Cuts at: 0 240 639 Size: 240 399 SacI G_AGCT'C Cuts at: 0 78 639 Size: 78 561 SanDI GG'GwC_CC Cuts at: 0 588 639 Size: 588 51 ScaI AGT'ACT Cuts at: 0 439 639 Size: 439 200 SfcI C'TryA_G Cuts at: 0 36 595 639 Size: 36 559 44 Fragments arranged by size: 559 44 36 SphI G_CATG'C Cuts at: 0 257 639 Size: 257 382 TatI w'GTAC_w Cuts at: 0 339 437 639 Size: 339 98 202 Fragments arranged by size: 339 202 98 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 60 453 639 Size: 60 393 186 Fragments arranged by size: 393 186 60 Enzymes that do cut: AarI Acc65I AleI AloI AlwNI ApoI BanI BanII BbsI BmrI BplI BpmI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI BspHI BspMI BstBI BstYI BtsI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI KpnI MslI MspA1I NspI PfoI PpuMI PstI PvuII SacI SanDI ScaI SfcI SphI TatI XcmI Enzymes that do not cut: AatII AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI