(Circular) (Six-base) MAPSORT of: pFN21AE1886.seq Check: 4452 from: 1 to: 5941 pFN21AE1886 5941 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3135 4004 5388 5656 1632 Size: 1503 869 1384 268 1917 Fragments arranged by size: 1917 1503 1384 869 268 AfeI AGC'GCT Cuts at: 2555 2555 Size: 5941 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2591 1184 Size: 1407 4534 AlwNI CAG_nnn'CTG Cuts at: 2212 2344 2986 3151 3684 5256 2212 Size: 132 642 165 533 1572 2897 Fragments arranged by size: 2897 1572 642 533 165 132 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 2324 2453 2750 3250 3476 3628 5737 1493 Size: 831 129 297 500 226 152 2109 1697 Fragments arranged by size: 2109 1697 831 500 297 226 152 129 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2232 2875 3266 1870 Size: 73 289 643 391 4545 Fragments arranged by size: 4545 643 391 289 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2470 2799 3262 4717 618 Size: 325 217 627 683 329 463 1455 1842 Fragments arranged by size: 1842 1455 683 627 463 329 325 217 BanII G_rGCy'C Cuts at: 1391 2029 2514 3159 3260 1391 Size: 638 485 645 101 4072 Fragments arranged by size: 4072 645 638 485 101 BbeI G_GCGC'C Cuts at: 2803 2803 Size: 5941 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4970 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3108 3142 4132 4166 1903 Size: 34 1171 34 990 34 3678 Fragments arranged by size: 3678 1171 990 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BfrBI ATG'CAT Cuts at: 2254 2254 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4552 136 Size: 107 122 71 631 605 2880 1525 Fragments arranged by size: 2880 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2142 2791 5936 2142 Size: 649 3145 2147 Fragments arranged by size: 3145 2147 649 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2475 2591 3144 4002 5158 1790 Size: 685 116 553 858 1156 2573 Fragments arranged by size: 2573 1156 858 685 553 116 BmgBI CAC'GTC Cuts at: 1318 2197 1318 Size: 879 5062 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3223 3223 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 3173 4601 1239 Size: 1025 909 1428 2579 Fragments arranged by size: 2579 1428 1025 909 Bpu10I CC'TnA_GC Cuts at: 2156 2175 3037 2156 Size: 19 862 5060 Fragments arranged by size: 5060 862 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2167 2789 4066 4931 5229 2167 Size: 622 1277 865 298 2879 Fragments arranged by size: 2879 1277 865 622 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 2744 5935 2744 Size: 3191 2750 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2800 3748 4130 4765 Size: 53 83 186 936 1267 948 382 635 Cuts at: 4765 275 Size: 1451 Fragments arranged by size: 1451 1267 948 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5716 Fragments arranged by size: 5716 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2954 1186 Size: 1075 693 4173 Fragments arranged by size: 4173 1075 693 BseYI C'CCAG_C Cuts at: 2480 3088 5144 5640 2480 Size: 608 2056 496 2781 Fragments arranged by size: 2781 2056 608 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2260 3084 1163 Size: 573 524 824 4020 Fragments arranged by size: 4020 824 573 524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2355 2514 2541 4002 4087 5158 2355 Size: 159 27 1461 85 1071 3138 Fragments arranged by size: 3138 1461 1071 159 85 27 BsmI GAATG_Cn' Cuts at: 2676 3452 3545 2676 Size: 776 93 5072 Fragments arranged by size: 5072 776 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2355 2475 2514 2541 2591 3144 Size: 399 239 326 120 39 27 50 553 Cuts at: 3144 3159 3260 4002 4087 5158 1391 Size: 15 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 553 399 326 239 120 101 85 50 39 27 15 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 2844 3830 5560 2844 Size: 986 1730 3225 Fragments arranged by size: 3225 1730 986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2541 4436 4610 65 Size: 1183 1293 1895 174 1396 Fragments arranged by size: 1895 1396 1293 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2862 4585 4772 1700 Size: 110 1052 1723 187 2869 Fragments arranged by size: 2869 1723 1052 187 110 BsrGI T'GTAC_A Cuts at: 96 3096 96 Size: 3000 2941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 2732 4001 5013 2732 Size: 1269 1012 3660 Fragments arranged by size: 3660 1269 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstXI CCAn_nnnn'nTGG Cuts at: 2786 3217 2786 Size: 431 5510 BstYI r'GATC_y Cuts at: 1972 2142 2520 2791 2983 3271 3638 4023 4040 Size: 170 378 271 192 288 367 385 17 Cuts at: 4040 5492 5578 5590 5936 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 378 367 346 288 271 192 170 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3012 3012 Size: 5941 BtgI C'CryG_G Cuts at: 513 1065 2058 2779 2785 513 Size: 552 993 721 6 3669 Fragments arranged by size: 3669 993 721 552 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2589 3067 3457 4251 4271 2589 Size: 478 390 794 20 4259 Fragments arranged by size: 4259 794 478 390 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DraIII CAC_nnn'GTG Cuts at: 2737 2737 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2101 2528 2782 Size: 54 1425 50 275 18 271 427 254 Cuts at: 2782 3388 4277 5906 8 Size: 606 889 1629 43 Fragments arranged by size: 1629 1425 889 606 427 275 271 254 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2111 2519 2641 3871 1353 Size: 758 408 122 1230 3423 Fragments arranged by size: 3423 1230 758 408 122 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2363 4528 4902 5048 387 Size: 1064 138 774 2165 374 146 1280 Fragments arranged by size: 2165 1280 1064 774 374 146 138 EcoICRI GAG'CTC Cuts at: 2512 2512 Size: 5941 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 3135 3173 4004 4601 5388 5656 Size: 393 632 871 38 831 597 787 268 Cuts at: 5656 1239 Size: 1524 Fragments arranged by size: 1524 871 831 787 632 597 393 268 38 EcoO109I rG'GnC_Cy Cuts at: 2209 3041 3148 3186 2209 Size: 832 107 38 4964 Fragments arranged by size: 4964 832 107 38 EcoRI G'AATT_C Cuts at: 2453 3250 3628 2453 Size: 797 378 4766 Fragments arranged by size: 4766 797 378 EcoRV GAT'ATC Cuts at: 2716 2716 Size: 5941 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2032 2032 Size: 5941 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2557 2803 5088 1678 Size: 879 246 2285 2531 Fragments arranged by size: 2531 2285 879 246 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2588 2620 4582 4614 4656 Size: 32 135 32 1016 32 1962 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 1962 1016 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2799 2799 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2277 2679 3001 3234 5055 5239 1305 Size: 972 402 322 233 1821 184 2007 Fragments arranged by size: 2007 1821 972 402 322 233 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4119 Fragments arranged by size: 4119 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2784 3218 3899 4258 4417 518 Size: 648 843 775 434 681 359 159 2042 Fragments arranged by size: 2042 843 775 681 648 434 359 159 MspA1I CmG'CkG Cuts at: 1606 3357 4034 5182 5427 1606 Size: 1751 677 1148 245 2120 Fragments arranged by size: 2120 1751 1148 677 245 NarI GG'CG_CC Cuts at: 2800 2800 Size: 5941 NcoI C'CATG_G Cuts at: 513 1065 2779 513 Size: 552 1714 3675 Fragments arranged by size: 3675 1714 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NsiI A_TGCA'T Cuts at: 2256 2256 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 3151 1108 Size: 1236 807 3898 Fragments arranged by size: 3898 1236 807 PfoI T'CCnGG_A Cuts at: 2718 2718 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2209 3148 3186 2209 Size: 939 38 4964 Fragments arranged by size: 4964 939 38 PshAI GACnn'nnGTC Cuts at: 1613 2195 1613 Size: 582 5359 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PstI C_TGCA'G Cuts at: 830 3293 830 Size: 2463 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 SacI G_AGCT'C Cuts at: 2514 2514 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SapI GCTCTTCn'nnn_ Cuts at: 2519 2519 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 3289 4424 5105 5296 826 Size: 219 2244 1135 681 191 1471 Fragments arranged by size: 2244 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2801 2801 Size: 5941 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2234 2877 3268 1872 Size: 362 643 391 4545 Fragments arranged by size: 4545 643 391 362 SmlI C'TyrA_G Cuts at: 820 1017 1943 2146 2804 4045 4946 5208 820 Size: 197 926 203 658 1241 901 262 1553 Fragments arranged by size: 1553 1241 926 901 658 262 203 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2091 2391 2779 3380 513 Size: 552 1026 300 388 601 3074 Fragments arranged by size: 3074 1026 601 552 388 300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2294 3968 3985 4138 4323 4768 325 Size: 1255 714 1674 17 153 185 445 1498 Fragments arranged by size: 1674 1498 1255 714 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3096 3171 4187 Size: 274 80 33 51 1443 1119 75 1016 Cuts at: 4187 5899 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1119 1016 274 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 2665 3277 2665 Size: 612 5329 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2232 2875 3266 1870 Size: 362 643 391 4545 Fragments arranged by size: 4545 643 391 362 XmnI GAAnn'nnTTC Cuts at: 2727 4070 2727 Size: 1343 4598 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I EcoNI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI