(Linear) (Six-base) MAPSORT of: KIEE1886.seq Check: 6865 from: 1 to: 1236 KIEE1886.orf With 182 enzymes: SgfI * July 15, 2010 18:49 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1130 1236 Size: 1130 106 AfeI AGC'GCT Cuts at: 0 550 1236 Size: 550 686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 586 1236 Size: 586 650 AlwNI CAG_nnn'CTG Cuts at: 0 207 339 981 1146 1236 Size: 207 132 642 165 90 Fragments arranged by size: 642 207 165 132 90 ApoI r'AATT_y Cuts at: 0 319 448 745 1236 Size: 319 129 297 491 Fragments arranged by size: 491 319 297 129 AvaI C'yCGr_G Cuts at: 0 227 870 1236 Size: 227 643 366 Fragments arranged by size: 643 366 227 BanI G'GyrC_C Cuts at: 0 465 794 1236 Size: 465 329 442 Fragments arranged by size: 465 442 329 BanII G_rGCy'C Cuts at: 0 24 509 1154 1236 Size: 24 485 645 82 Fragments arranged by size: 645 485 82 24 BbeI G_GCGC'C Cuts at: 0 798 1236 Size: 798 438 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1103 1137 1236 Size: 1103 34 99 Fragments arranged by size: 1103 99 34 BfrBI ATG'CAT Cuts at: 0 249 1236 Size: 249 987 BglII A'GATC_T Cuts at: 0 137 786 1236 Size: 137 649 450 Fragments arranged by size: 649 450 137 Bme1580I G_kGCm'C Cuts at: 0 470 586 1139 1236 Size: 470 116 553 97 Fragments arranged by size: 553 470 116 97 BmgBI CAC'GTC Cuts at: 0 192 1236 Size: 192 1044 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1218 1236 Size: 1218 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 1168 1236 Size: 259 909 68 Fragments arranged by size: 909 259 68 Bpu10I CC'TnA_GC Cuts at: 0 151 170 1032 1236 Size: 151 19 862 204 Fragments arranged by size: 862 204 151 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 162 784 1236 Size: 162 622 452 Fragments arranged by size: 622 452 162 BsaBI GATnn'nnATC Cuts at: 0 739 1236 Size: 739 497 BsaHI Gr'CG_yC Cuts at: 0 795 1236 Size: 795 441 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 256 949 1236 Size: 256 693 287 Fragments arranged by size: 693 287 256 BseYI C'CCAG_C Cuts at: 0 475 1083 1236 Size: 475 608 153 Fragments arranged by size: 608 475 153 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 255 1079 1236 Size: 255 824 157 Fragments arranged by size: 824 255 157 BsiHKAI G_wGCw'C Cuts at: 0 350 509 536 1236 Size: 350 159 27 700 Fragments arranged by size: 700 350 159 27 BsmI GAATG_Cn' Cuts at: 0 671 1236 Size: 671 565 Bsp1286I G_dGCh'C Cuts at: 0 24 350 470 509 536 586 1139 1154 Size: 24 326 120 39 27 50 553 15 Cuts at: 1154 1236 Size: 82 Fragments arranged by size: 553 326 120 82 50 39 27 24 15 BspHI T'CATG_A Cuts at: 0 839 1236 Size: 839 397 BsrDI GCAATG_nn' Cuts at: 0 536 1236 Size: 536 700 BsrFI r'CCGG_y Cuts at: 0 857 1236 Size: 857 379 BsrGI T'GTAC_A Cuts at: 0 1091 1236 Size: 1091 145 BssSI C'ACGA_G Cuts at: 0 727 1236 Size: 727 509 BstXI CCAn_nnnn'nTGG Cuts at: 0 781 1212 1236 Size: 781 431 24 Fragments arranged by size: 781 431 24 BstYI r'GATC_y Cuts at: 0 137 515 786 978 1236 Size: 137 378 271 192 258 Fragments arranged by size: 378 271 258 192 137 Bsu36I CC'TnA_GG Cuts at: 0 1007 1236 Size: 1007 229 BtgI C'CryG_G Cuts at: 0 53 774 780 1236 Size: 53 721 6 456 Fragments arranged by size: 721 456 53 6 BtsI GCAGTG_nn' Cuts at: 0 584 1062 1236 Size: 584 478 174 Fragments arranged by size: 584 478 174 DraIII CAC_nnn'GTG Cuts at: 0 732 1236 Size: 732 504 EaeI y'GGCC_r Cuts at: 0 96 523 777 1236 Size: 96 427 254 459 Fragments arranged by size: 459 427 254 96 EarI CTCTTCn'nnn_ Cuts at: 0 106 514 636 1236 Size: 106 408 122 600 Fragments arranged by size: 600 408 122 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 358 1236 Size: 358 878 EcoICRI GAG'CTC Cuts at: 0 507 1236 Size: 507 729 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 1130 1168 1236 Size: 259 871 38 68 Fragments arranged by size: 871 259 68 38 EcoO109I rG'GnC_Cy Cuts at: 0 204 1036 1143 1181 1236 Size: 204 832 107 38 55 Fragments arranged by size: 832 204 107 55 38 EcoRI G'AATT_C Cuts at: 0 448 1236 Size: 448 788 EcoRV GAT'ATC Cuts at: 0 711 1236 Size: 711 525 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 27 1236 Size: 27 1209 HaeII r_GCGC'y Cuts at: 0 552 798 1236 Size: 552 246 438 Fragments arranged by size: 552 438 246 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 583 615 1236 Size: 583 32 621 Fragments arranged by size: 621 583 32 KasI G'GCGC_C Cuts at: 0 794 1236 Size: 794 442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 272 674 996 1229 1236 Size: 272 402 322 233 7 Fragments arranged by size: 402 322 272 233 7 MslI CAynn'nnrTG Cuts at: 0 779 1213 1236 Size: 779 434 23 Fragments arranged by size: 779 434 23 NarI GG'CG_CC Cuts at: 0 795 1236 Size: 795 441 NcoI C'CATG_G Cuts at: 0 774 1236 Size: 774 462 NsiI A_TGCA'T Cuts at: 0 251 1236 Size: 251 985 PflMI CCAn_nnn'nTGG Cuts at: 0 339 1146 1236 Size: 339 807 90 Fragments arranged by size: 807 339 90 PfoI T'CCnGG_A Cuts at: 0 713 1236 Size: 713 523 PpuMI rG'GwC_Cy Cuts at: 0 204 1143 1181 1236 Size: 204 939 38 55 Fragments arranged by size: 939 204 55 38 PshAI GACnn'nnGTC Cuts at: 0 190 1236 Size: 190 1046 SacI G_AGCT'C Cuts at: 0 509 1236 Size: 509 727 SapI GCTCTTCn'nnn_ Cuts at: 0 514 1236 Size: 514 722 SfoI GGC'GCC Cuts at: 0 796 1236 Size: 796 440 SmaI CCC'GGG Cuts at: 0 229 872 1236 Size: 229 643 364 Fragments arranged by size: 643 364 229 SmlI C'TyrA_G Cuts at: 0 141 799 1236 Size: 141 658 437 Fragments arranged by size: 658 437 141 StyI C'CwwG_G Cuts at: 0 86 386 774 1236 Size: 86 300 388 462 Fragments arranged by size: 462 388 300 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 289 1236 Size: 289 947 TatI w'GTAC_w Cuts at: 0 1091 1166 1236 Size: 1091 75 70 Fragments arranged by size: 1091 75 70 XbaI T'CTAG_A Cuts at: 0 660 1236 Size: 660 576 XmaI C'CCGG_G Cuts at: 0 227 870 1236 Size: 227 643 366 Fragments arranged by size: 643 366 227 XmnI GAAnn'nnTTC Cuts at: 0 722 1236 Size: 722 514 Enzymes that do cut: AcuI AfeI AlfI AlwNI ApoI AvaI BanI BanII BbeI BcgI BfrBI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I KasI MmeI MslI NarI NcoI NsiI PflMI PfoI PpuMI PshAI SacI SapI SfoI SmaI SmlI StyI TaqII TatI XbaI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BglI BlpI BmrI BmtI BsaI BsaAI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoNI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI MspA1I NaeI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XcmI XhoI ZraI