################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BbeI BbrPI BcgI BciVI BcnI BfrI BglII BlnI BpmI BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MroI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcsI 1014 GAATTT [GA]^AATT[CT] AflIII 730 ACATGT A^C[GA][CT]GT AgeI 555 ACCGGT A^CCGGT ApoI 1014 GAATTT [GA]^AATT[CT] AvaI 26 CTCGGG C^[CT]CG[GA]G BalI 962 TGGCCA TGG^CCA BamHI 1332 GGATCC G^GATCC BanII 788 GGGCTC G[GA]GC[CT]^C BbsI 160 GTCTTC GAAGAC(2/6) BbvCI 860 CCTCAGC CCTCAGC(-5/-2) BclI 1266 TGATCA T^GATCA BglI 457 GCCAAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 160 GTCTTC GAAGAC(2/6) BsaAI 1249 TACGTG [CT]AC^GT[GA] BsaI 99 GGTCTC GGTCTC(1/5) BsaWI 555 ACCGGT [AT]^CCGG[AT] BseRI 1345 CTCCTC GAGGAG(10/8) BsgI 337 GTGCAG GTGCAG(16/14) BsmBI 81 GAGACG CGTCTC(1/5) BsoBI 26 CTCGGG C^[CT]CG[GA]G BspLU11I 730 ACATGT A^CATGT BspMI 339 GCAGGT ACCTGC(4/8) BsrBI 39 CCGCTC CCGCTC(-3/-3) BstEII 121 GGTTACC G^GT[ACGT]ACC BstPI 121 GGTTACC G^GT[ACGT]ACC BstXI 917 CCAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 696 GCGC GCG^C DsaI 970 CCACGG C^C[GA][CT]GG EaeI 962 TGGCCA [CT]^GGCC[GA] EcoO65I 121 GGTTACC G^GT[ACGT]ACC EcoT22I 1186 ATGCAT ATGCA^T FauI 14 CCCGC CCCGC(4/6) FbaI 1266 TGATCA T^GATCA HgaI 708 GCGTC GACGC(5/10) HhaI 696 GCGC GCG^C HinP1I 696 GCGC G^CGC MluNI 962 TGGCCA TGG^CCA MscI 962 TGGCCA TGG^CCA MslI 543 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 462 GCCGGC GCC^GGC NgoMIV 462 GCCGGC G^CCGGC NsiI 1186 ATGCAT ATGCA^T NspI 730 ACATGT [GA]CATG^[CT] PinAI 555 ACCGGT A^CCGGT PleI 1340 GAGTC GAGTC(4/5) PvuII 188 CAGCTG CAG^CTG SexAI 117 ACCAGGT A^CC[AT]GGT SgrAI 554 CACCGGTG C[GA]^CCGG[CT]G StuI 857 AGGCCT AGG^CCT TaqI 401 TCGA T^CGA TfiI 238 GAATC G^A[AT]TC Tsp509I 1015 AATT ^AATT TthHB8I 401 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Acc65I 381 GGTACC G^GTACC 1099 GGTACC Asp718I 381 GGTACC G^GTACC 1099 GGTACC AvaII 115 GGACC G^G[AT]CC 1034 GGACC BlpI 631 GCTGAGC GC^T[ACGT]AGC 703 GCTCAGC BmyI 559 GTGCCC G[AGT]GC[ACT]^C 788 GGGCTC Bpu1102I 631 GCTGAGC GC^T[ACGT]AGC 703 GCTCAGC BsmFI 114 GGGAC GGGAC(10/14) 6 GTCCC Bsp1286I 559 GTGCCC G[AGT]GC[ACT]^C 788 GGGCTC BstYI 1332 GGATCC [GA]^GATC[CT] 1353 GGATCT CelII 631 GCTGAGC GC^T[ACGT]AGC 703 GCTCAGC HinfI 238 GAATC G^A[ACGT]TC 1340 GAGTC KpnI 381 GGTACC GGTAC^C 1099 GGTACC MaeII 84 ACGT A^CGT 1250 ACGT MflI 1332 GGATCC [GA]^GATC[CT] 1353 GGATCT MseI 149 TTAA T^TAA 752 TTAA PflMI 352 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 637 CCAGGAGCTGG Tru9I 149 TTAA T^TAA 752 TTAA Tsp45I 244 GTCAC ^GT[GC]AC 1297 GTGAC Van91I 352 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 637 CCAGGAGCTGG XhoII 1332 GGATCC [GA]^GATC[CT] 1353 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BanI BbvI BfaI BsaJI BsiYI BslI BsmAI BsrDI BsrFI BsrI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI XcmI