(Circular) (Six-base) MAPSORT of: pFN21AE4436.seq Check: 666 from: 1 to: 6064 pFN21AE4436 6064 bp With 182 enzymes: SgfI * March 28, 2012 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3407 1937 Size: 1470 4594 Acc65I G'GTAC_C Cuts at: 2386 3104 3385 2386 Size: 718 281 5065 Fragments arranged by size: 5065 718 281 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2152 2493 2738 4127 5511 5779 1632 Size: 520 341 245 1389 1384 268 1917 Fragments arranged by size: 1917 1389 1384 520 341 268 245 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 2735 4963 5940 1203 Size: 1532 2228 977 1327 Fragments arranged by size: 2228 1532 1327 977 AgeI A'CCGG_T Cuts at: 2560 4895 2560 Size: 2335 3729 AhdI GACnn_n'nnGTC Cuts at: 4793 4793 Size: 6064 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2675 2721 1184 Size: 1491 46 4527 Fragments arranged by size: 4527 1491 46 AlwNI CAG_nnn'CTG Cuts at: 2246 2648 2716 3807 5379 2246 Size: 402 68 1091 1572 2931 Fragments arranged by size: 2931 1572 1091 402 68 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 3019 3373 3599 3751 5860 1493 Size: 1526 354 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 354 226 152 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 2031 3389 1870 Size: 73 88 1358 4545 Fragments arranged by size: 4545 1358 88 73 BamHI G'GATC_C Cuts at: 3337 3394 3761 3337 Size: 57 367 5640 Fragments arranged by size: 5640 367 57 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2386 2563 3104 3385 4840 Size: 325 217 627 599 177 541 281 1455 Cuts at: 4840 618 Size: 1842 Fragments arranged by size: 1842 1455 627 599 541 325 281 217 177 BanII G_rGCy'C Cuts at: 1391 2797 3383 1391 Size: 1406 586 4072 Fragments arranged by size: 4072 1406 586 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2158 928 Size: 971 259 4834 Fragments arranged by size: 4834 971 259 BbvCI CC'TCA_GC Cuts at: 2866 2866 Size: 6064 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4255 4289 1903 Size: 34 50 34 2234 34 3678 Fragments arranged by size: 3678 2234 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3956 5166 3956 Size: 1210 4854 BclI T'GATC_A Cuts at: 1548 3271 1548 Size: 1723 4341 BfrBI ATG'CAT Cuts at: 3193 3193 Size: 6064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2468 4675 136 Size: 107 122 71 631 605 796 2207 1525 Fragments arranged by size: 2207 1525 796 631 605 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 2637 2709 3481 2637 Size: 72 772 5220 Fragments arranged by size: 5220 772 72 Bme1580I G_kGCm'C Cuts at: 1790 2568 4125 5281 1790 Size: 778 1557 1156 2573 Fragments arranged by size: 2573 1557 1156 778 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3062 3795 4753 Size: 416 53 83 399 24 1645 733 958 Cuts at: 4753 442 Size: 1753 Fragments arranged by size: 1753 1645 958 733 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3368 3368 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4724 1239 Size: 3485 2579 Bpu10I CC'TnA_GC Cuts at: 2457 2866 2457 Size: 409 5655 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4189 5054 5352 4189 Size: 865 298 4901 Fragments arranged by size: 4901 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2110 4727 882 Size: 617 611 2617 2219 Fragments arranged by size: 2617 2219 617 611 BsaAI yAC'GTr Cuts at: 493 1204 3256 3855 493 Size: 711 2052 599 2702 Fragments arranged by size: 2702 2052 711 599 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3871 4253 4888 275 Size: 53 83 186 936 2338 382 635 1451 Fragments arranged by size: 2338 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2560 4497 4895 5169 5316 5727 2560 Size: 1937 398 274 147 411 2897 Fragments arranged by size: 2897 1937 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2277 2307 3216 3246 1178 Size: 30 165 30 874 30 909 30 3996 Fragments arranged by size: 3996 909 874 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3341 1186 Size: 2155 3909 BseYI C'CCAG_C Cuts at: 2230 5267 5763 2230 Size: 3037 496 2531 Fragments arranged by size: 3037 2531 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2363 1163 Size: 573 627 4864 Fragments arranged by size: 4864 627 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4125 4210 5281 4125 Size: 85 1071 4908 Fragments arranged by size: 4908 1071 85 BsmI GAATG_Cn' Cuts at: 3575 3668 3575 Size: 93 5971 BsmBI CGTCTCn'nnnn_ Cuts at: 2080 2080 Size: 6064 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2568 2797 3383 4125 4210 5281 1391 Size: 399 778 229 586 742 85 1071 2174 Fragments arranged by size: 2174 1071 778 742 586 399 229 85 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 3953 5683 3953 Size: 1730 4334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2335 3419 844 Size: 1491 1084 3489 Fragments arranged by size: 3489 1491 1084 BsrBI CCG'CTC Cuts at: 1394 1823 2046 3951 1394 Size: 429 223 1905 3507 Fragments arranged by size: 3507 1905 429 223 BsrDI GCAATG_nn' Cuts at: 65 1248 2703 2748 3127 4559 4733 65 Size: 1183 1455 45 379 1432 174 1396 Fragments arranged by size: 1455 1432 1396 1183 379 174 45 BsrFI r'CCGG_y Cuts at: 1700 1810 2467 2560 3276 4708 4895 1700 Size: 110 657 93 716 1432 187 2869 Fragments arranged by size: 2869 1432 716 657 187 110 93 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 4124 5136 4124 Size: 1012 5052 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6064 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 2126 1184 Size: 942 5122 BstXI CCAn_nnnn'nTGG Cuts at: 2929 2929 Size: 6064 BstYI r'GATC_y Cuts at: 1972 3337 3358 3394 3761 4146 4163 5615 5701 Size: 1365 21 36 367 385 17 1452 86 Cuts at: 5701 5713 6059 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1365 385 367 346 86 36 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2975 513 Size: 552 1910 3602 Fragments arranged by size: 3602 1910 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5325 Fragments arranged by size: 5325 683 56 BtsI GCAGTG_nn' Cuts at: 2910 2970 3580 4374 4394 2910 Size: 60 610 794 20 4580 Fragments arranged by size: 4580 794 610 60 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2967 3511 4400 Size: 54 1425 50 275 18 1137 544 889 Cuts at: 4400 6029 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1137 889 544 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3994 1353 Size: 2641 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2044 4651 5025 5171 387 Size: 1064 138 455 2607 374 146 1280 Fragments arranged by size: 2607 1280 1064 455 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2152 2493 2738 4127 4724 5511 5779 Size: 393 520 341 245 1389 597 787 268 Cuts at: 5779 1239 Size: 1524 Fragments arranged by size: 1524 1389 787 597 520 393 341 268 245 EcoRI G'AATT_C Cuts at: 3373 3751 3373 Size: 378 5686 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2227 2227 Size: 6064 FspI TGC'GCA Cuts at: 4570 4570 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 5211 1678 Size: 3533 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 2362 2394 2779 Size: 32 135 32 749 32 9 32 385 Cuts at: 2779 2811 3256 3288 4705 4737 4779 4811 1373 Size: 32 445 32 1417 32 42 32 2626 Fragments arranged by size: 2626 1417 749 445 385 135 42 32 32 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 3408 3654 669 Size: 988 281 1470 246 3079 Fragments arranged by size: 3079 1470 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 3654 3654 Size: 6064 KpnI G_GTAC'C Cuts at: 2390 3108 3389 2390 Size: 718 281 5065 Fragments arranged by size: 5065 718 281 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2263 5178 5362 1305 Size: 958 2915 184 2007 Fragments arranged by size: 2915 2007 958 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2969 10 Size: 54 1425 343 1137 3105 Fragments arranged by size: 3105 1425 1137 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 4022 4381 4540 518 Size: 648 1386 1470 359 159 2042 Fragments arranged by size: 2042 1470 1386 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2195 2516 3329 3480 4157 5305 5550 Size: 416 173 321 813 151 677 1148 245 Cuts at: 5550 1606 Size: 2120 Fragments arranged by size: 2120 1148 813 677 416 321 245 173 151 NaeI GCC'GGC Cuts at: 2469 2469 Size: 6064 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5512 NdeI CA'TA_TG Cuts at: 387 387 Size: 6064 NgoMIV G'CCGG_C Cuts at: 2467 2467 Size: 6064 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NsiI A_TGCA'T Cuts at: 3195 3195 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2739 3422 4967 1132 Size: 97 1510 683 1545 2229 Fragments arranged by size: 2229 1545 1510 683 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 2735 4963 2735 Size: 2228 3836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2363 2648 1108 Size: 1255 285 4524 Fragments arranged by size: 4524 1255 285 PfoI T'CCnGG_A Cuts at: 2781 3354 2781 Size: 573 5491 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6064 PsiI TTA'TAA Cuts at: 3634 3634 Size: 6064 PstI C_TGCA'G Cuts at: 830 2491 2536 2608 3416 830 Size: 1661 45 72 808 3478 Fragments arranged by size: 3478 1661 808 72 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 PvuII CAG'CTG Cuts at: 2195 2195 Size: 6064 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SexAI A'CCwGG_T Cuts at: 2122 2122 Size: 6064 SfcI C'TryA_G Cuts at: 826 1045 2190 2487 2508 2532 2604 3219 3412 Size: 219 1145 297 21 24 72 615 193 Cuts at: 3412 4547 5228 5419 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1145 1135 681 615 297 219 193 191 72 24 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 2560 1700 Size: 860 5204 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4168 5069 5331 820 Size: 197 926 2225 901 262 1553 Fragments arranged by size: 2225 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 3422 1132 Size: 2290 3774 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 2864 1517 Size: 1347 4717 StyI C'CwwG_G Cuts at: 513 1065 3503 513 Size: 552 2438 3074 Fragments arranged by size: 3074 2438 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2554 3169 4091 4108 4261 4446 4891 Size: 1255 974 615 922 17 153 185 445 Cuts at: 4891 325 Size: 1498 Fragments arranged by size: 1498 1255 974 922 615 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4310 6022 96 Size: 274 80 33 51 1443 2333 1712 138 Fragments arranged by size: 2333 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2428 2460 6024 6056 2428 Size: 32 3564 32 2436 Fragments arranged by size: 3564 2436 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6064 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3049 3091 3253 3049 Size: 42 162 5860 Fragments arranged by size: 5860 162 42 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6064 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 4193 4193 Size: 6064 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI