Restriction map for
pFN21AE4436
(Linear) (Six-base) MAPSORT of: KIEE4436.seq Check: 4765 from: 1 to: 1359
KIEE4436.orf
With 182 enzymes: SgfI *
March 22, 2012 18:01 ..
Acc65I G'GTAC_C
Cuts at: 0 381 1099 1359
Size: 381 718 260
Fragments arranged by size:
718 381 260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 488 733 1359
Size: 147 341 245 626
Fragments arranged by size:
626 341 245 147
AflIII A'CryG_T
Cuts at: 0 730 1359
Size: 730 629
AgeI A'CCGG_T
Cuts at: 0 555 1359
Size: 555 804
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 670 716 1359
Size: 670 46 643
Fragments arranged by size:
670 643 46
AlwNI CAG_nnn'CTG
Cuts at: 0 241 643 711 1359
Size: 241 402 68 648
Fragments arranged by size:
648 402 241 68
ApoI r'AATT_y
Cuts at: 0 1014 1359
Size: 1014 345
AvaI C'yCGr_G
Cuts at: 0 26 1359
Size: 26 1333
BamHI G'GATC_C
Cuts at: 0 1332 1359
Size: 1332 27
BanI G'GyrC_C
Cuts at: 0 381 558 1099 1359
Size: 381 177 541 260
Fragments arranged by size:
541 381 260 177
BanII G_rGCy'C
Cuts at: 0 792 1359
Size: 792 567
BbsI GAAGACnn'nnnn_
Cuts at: 0 153 1359
Size: 153 1206
BbvCI CC'TCA_GC
Cuts at: 0 861 1359
Size: 861 498
BclI T'GATC_A
Cuts at: 0 1266 1359
Size: 1266 93
BfrBI ATG'CAT
Cuts at: 0 1188 1359
Size: 1188 171
BglI GCCn_nnn'nGGC
Cuts at: 0 463 1359
Size: 463 896
BlpI GC'TnA_GC
Cuts at: 0 632 704 1359
Size: 632 72 655
Fragments arranged by size:
655 632 72
Bme1580I G_kGCm'C
Cuts at: 0 563 1359
Size: 563 796
BmrI ACTGGGnnnn_n'
Cuts at: 0 1057 1359
Size: 1057 302
Bpu10I CC'TnA_GC
Cuts at: 0 452 861 1359
Size: 452 409 498
Fragments arranged by size:
498 452 409
BsaI GGTCTCn'nnnn_
Cuts at: 0 105 1359
Size: 105 1254
BsaAI yAC'GTr
Cuts at: 0 1251 1359
Size: 1251 108
BsaWI w'CCGG_w
Cuts at: 0 555 1359
Size: 555 804
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 272 302 1211 1241 1359
Size: 272 30 909 30 118
Fragments arranged by size:
909 272 118 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1336 1359
Size: 1336 23
BseYI C'CCAG_C
Cuts at: 0 225 1359
Size: 225 1134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1359
Size: 358 1001
BsmBI CGTCTCn'nnnn_
Cuts at: 0 75 1359
Size: 75 1284
Bsp1286I G_dGCh'C
Cuts at: 0 563 792 1359
Size: 563 229 567
Fragments arranged by size:
567 563 229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 330 1359
Size: 330 1029
BsrBI CCG'CTC
Cuts at: 0 41 1359
Size: 41 1318
BsrDI GCAATG_nn'
Cuts at: 0 698 743 1122 1359
Size: 698 45 379 237
Fragments arranged by size:
698 379 237 45
BsrFI r'CCGG_y
Cuts at: 0 462 555 1271 1359
Size: 462 93 716 88
Fragments arranged by size:
716 462 93 88
BstEII G'GTnAC_C
Cuts at: 0 121 1359
Size: 121 1238
BstXI CCAn_nnnn'nTGG
Cuts at: 0 924 1359
Size: 924 435
BstYI r'GATC_y
Cuts at: 0 1332 1353 1359
Size: 1332 21 6
Fragments arranged by size:
1332 21 6
BtgI C'CryG_G
Cuts at: 0 970 1359
Size: 970 389
BtsI GCAGTG_nn'
Cuts at: 0 905 965 1359
Size: 905 60 394
Fragments arranged by size:
905 394 60
EaeI y'GGCC_r
Cuts at: 0 962 1359
Size: 962 397
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 39 1359
Size: 39 1320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 488 733 1359
Size: 147 341 245 626
Fragments arranged by size:
626 341 245 147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 222 1359
Size: 222 1137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 357 389 774 806 1251 1283
Size: 316 32 9 32 385 32 445 32
Cuts at: 1283 1359
Size: 76
Fragments arranged by size:
445 385 316 76 32 32 32 32
9
KpnI G_GTAC'C
Cuts at: 0 385 1103 1359
Size: 385 718 256
Fragments arranged by size:
718 385 256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1359
Size: 258 1101
MscI TGG'CCA
Cuts at: 0 964 1359
Size: 964 395
MslI CAynn'nnrTG
Cuts at: 0 547 1359
Size: 547 812
MspA1I CmG'CkG
Cuts at: 0 17 190 511 1324 1359
Size: 17 173 321 813 35
Fragments arranged by size:
813 321 173 35 17
NaeI GCC'GGC
Cuts at: 0 464 1359
Size: 464 895
NgoMIV G'CCGG_C
Cuts at: 0 462 1359
Size: 462 897
NsiI A_TGCA'T
Cuts at: 0 1190 1359
Size: 1190 169
NspI r_CATG'y
Cuts at: 0 734 1359
Size: 734 625
PciI A'CATG_T
Cuts at: 0 730 1359
Size: 730 629
PflMI CCAn_nnn'nTGG
Cuts at: 0 358 643 1359
Size: 358 285 716
Fragments arranged by size:
716 358 285
PfoI T'CCnGG_A
Cuts at: 0 776 1349 1359
Size: 776 573 10
Fragments arranged by size:
776 573 10
PstI C_TGCA'G
Cuts at: 0 486 531 603 1359
Size: 486 45 72 756
Fragments arranged by size:
756 486 72 45
PvuII CAG'CTG
Cuts at: 0 190 1359
Size: 190 1169
SexAI A'CCwGG_T
Cuts at: 0 117 1359
Size: 117 1242
SfcI C'TryA_G
Cuts at: 0 185 482 503 527 599 1214 1359
Size: 185 297 21 24 72 615 145
Fragments arranged by size:
615 297 185 145 72 24 21
SgrAI Cr'CCGG_yG
Cuts at: 0 555 1359
Size: 555 804
StuI AGG'CCT
Cuts at: 0 859 1359
Size: 859 500
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 549 1164 1359
Size: 549 615 195
Fragments arranged by size:
615 549 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 423 455 1359
Size: 423 32 904
Fragments arranged by size:
904 423 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1044 1086 1248 1359
Size: 1044 42 162 111
Fragments arranged by size:
1044 162 111 42
Enzymes that do cut:
Acc65I AcuI AflIII AgeI AlfI AlwNI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BclI BfrBI BglI
BlpI Bme1580I BmrI Bpu10I BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstEII BstXI BstYI BtgI BtsI EaeI EciI
Eco57MI FalI Hin4I KpnI MmeI MscI MslI MspA1I
NaeI NgoMIV NsiI NspI PciI PflMI PfoI PstI
PvuII SexAI SfcI SgrAI StuI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BglII BmgBI BmtI BplI BpmI
BpuEI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI NarI
NcoI NdeI NheI NotI NruI PacI PasI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StyI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI