################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI ApoI AscI AseI AsnI Asp718I AspEI AvaII AviII AvrII BamHI BbrPI BbvCI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI Ksp632I MaeIII MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI RcaI RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tsp509I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 520 GACGTC GACGT^C Alw44I 84 GTGCAC G^TGCAC AlwI 108 GATCC GGATC(4/5) AlwNI 823 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 84 GTGCAC G^TGCAC Asp700I 492 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 966 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 70 GGCGCC G^G[CT][GA]CC BbeI 70 GGCGCC GGCGC^C BciVI 572 GGATAC GTATCC(6/5) BfaI 478 CTAG C^TAG BglI 240 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiEI 393 CGATCG CG[GA][CT]^CG BsmAI 43 GAGAC GTCTC(1/5) BsmFI 964 GGGAC GGGAC(10/14) BsmI 496 GCATTC GAATGC(1/-1) BsrBI 859 GAGCGG CCGCTC(-3/-3) BsrDI 915 CATTGC GCAATG(2/0) BsrFI 1041 GCCGGC [GA]^CCGG[CT] BssHII 190 GCGCGC G^CGCGC BstYI 107 AGATCC [GA]^GATC[CT] Cfr10I 1041 GCCGGC [GA]^CCGG[CT] DraI 417 TTTAAA TTT^AAA KasI 70 GGCGCC G^GCGCC MaeI 478 CTAG C^TAG MaeII 521 ACGT A^CGT MflI 107 AGATCC [GA]^GATC[CT] MseI 418 TTAA T^TAA NaeI 1041 GCCGGC GCC^GGC NarI 70 GGCGCC GG^CGCC NgoMIV 1041 GCCGGC G^CCGGC PflFI 966 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 393 CGATCG CGAT^CG PvuII 1066 CAGCTG CAG^CTG SacI 135 GAGCTC GAGCT^C SfoI 70 GGCGCC GGC^GCC SmlI 709 CTCAAG C^T[CT][GA]AG TfiI 370 GATTC G^A[AT]TC Tru9I 418 TTAA T^TAA Tth111I 966 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 107 AGATCC [GA]^GATC[CT] XmnI 492 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 70 GGCGCC G[GA]^CG[CT]C 520 GACGTC AfaI 59 GTAC GT^AC 998 GTAC Aor51HI 21 AGCGCT AGC^GCT 153 AGCGCT ApaI 238 GGGCCC GGGCC^C 864 GGGCCC BalI 50 TGGCCA TGG^CCA 542 TGGCCA BbsI 565 GAAGAC GAAGAC(2/6) 1033 GAAGAC BcgI 86 GCACTGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 787 GCAGCCGAGTCG BlpI 24 GCTGAGC GC^T[ACGT]AGC 201 GCTCAGC Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC 201 GCTCAGC BpuAI 565 GAAGAC GAAGAC(2/6) 1033 GAAGAC BsaHI 70 GGCGCC G[GA]^CG[CT]C 520 GACGTC BsrI 88 ACTGG ACTGG(1/-1) 756 ACTGG BstXI 272 CCACCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 953 CCAAAGTGCTGG CelII 24 GCTGAGC GC^T[ACGT]AGC 201 GCTCAGC DpnII 108 GATC ^GATC 394 GATC Eco47III 21 AGCGCT AGC^GCT 153 AGCGCT HgaI 871 GACGC GACGC(5/10) 305 GCGTC Hin1I 70 GGCGCC G[GA]^CG[CT]C 520 GACGTC KspI 145 CCGCGG CCGC^GG 209 CCGCGG MboI 108 GATC ^GATC 394 GATC MluNI 50 TGGCCA TGG^CCA 542 TGGCCA MscI 50 TGGCCA TGG^CCA 542 TGGCCA MslI 915 CATTGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 951 CACCAAAGTG NcoI 671 CCATGG C^CATGG 1025 CCATGG NdeII 108 GATC ^GATC 394 GATC RsaI 59 GTAC GT^AC 998 GTAC SacII 145 CCGCGG CCGC^GG 209 CCGCGG Sau3AI 108 GATC ^GATC 394 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AluI AspHI AvaI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI Sau96I ScrFI SfaNI SmaI StyI TaqI TseI TspRI TthHB8I XmaCI XmaI