(Circular) (Six-base) MAPSORT of: pFN21AB7616.seq Check: 7397 from: 1 to: 5788 pFN21AB7616 5788 bp With 182 enzymes: SgfI * March 28, 2012 12:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2529 3598 278 Size: 53 83 186 936 993 1069 2468 Fragments arranged by size: 2468 1069 993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2400 3851 5235 5503 1632 Size: 768 1451 1384 268 1917 Fragments arranged by size: 1917 1451 1384 768 268 AfeI AGC'GCT Cuts at: 2028 2160 2028 Size: 132 5656 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 2960 1166 Size: 1794 3994 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2942 2978 1184 Size: 1758 36 3994 Fragments arranged by size: 3994 1758 36 AlwNI CAG_nnn'CTG Cuts at: 2833 3531 5103 2833 Size: 698 1572 3518 Fragments arranged by size: 3518 1572 698 ApaI G_GGCC'C Cuts at: 2247 2873 2247 Size: 626 5162 ApaLI G'TGCA_C Cuts at: 2089 3845 5001 2089 Size: 1756 1156 2876 Fragments arranged by size: 2876 1756 1156 ApoI r'AATT_y Cuts at: 1493 3097 3323 3475 5584 1493 Size: 1604 226 152 2109 1697 Fragments arranged by size: 2109 1697 1604 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2137 2177 2246 2535 2702 2813 Size: 73 125 69 40 69 289 167 111 Cuts at: 2813 3113 1870 Size: 300 4545 Fragments arranged by size: 4545 300 289 167 125 111 73 69 69 40 BamHI G'GATC_C Cuts at: 3118 3485 3118 Size: 367 5421 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 3109 4564 618 Size: 325 217 627 288 1034 1455 1842 Fragments arranged by size: 1842 1455 1034 627 325 288 217 BanII G_rGCy'C Cuts at: 1391 2136 2144 2247 2256 2667 2873 3107 1391 Size: 745 8 103 9 411 206 234 4072 Fragments arranged by size: 4072 745 411 234 206 103 9 8 BbeI G_GCGC'C Cuts at: 2079 2079 Size: 5788 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2577 3045 928 Size: 971 678 468 3671 Fragments arranged by size: 3671 971 678 468 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2080 2114 2781 2815 3979 4013 1903 Size: 34 143 34 667 34 1164 34 3678 Fragments arranged by size: 3678 1164 667 143 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2571 3680 4890 2571 Size: 1109 1210 3469 Fragments arranged by size: 3469 1210 1109 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2251 4399 136 Size: 107 122 71 631 605 579 2148 1525 Fragments arranged by size: 2148 1525 631 605 579 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 2030 2207 3205 2030 Size: 177 998 4613 Fragments arranged by size: 4613 998 177 Bme1580I G_kGCm'C Cuts at: 1790 2093 2184 2247 2752 2873 3849 5005 1790 Size: 303 91 63 505 121 976 1156 2573 Fragments arranged by size: 2573 1156 976 505 303 121 91 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2103 2771 3519 Size: 416 53 83 399 24 686 668 748 Cuts at: 3519 4477 442 Size: 958 1753 Fragments arranged by size: 1753 958 748 686 668 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2377 2513 2804 3095 4448 1239 Size: 1138 136 291 291 1353 2579 Fragments arranged by size: 2579 1353 1138 291 291 136 Bpu10I CC'TnA_GC Cuts at: 2880 2943 2880 Size: 63 5725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2699 3913 4778 5076 2699 Size: 1214 865 298 3411 Fragments arranged by size: 3411 1214 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4451 882 Size: 617 2952 2219 Fragments arranged by size: 2952 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3579 493 Size: 711 2375 2702 Fragments arranged by size: 2702 2375 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 2526 3595 3977 Size: 53 83 186 936 543 450 1069 382 Cuts at: 3977 4612 275 Size: 635 1451 Fragments arranged by size: 1451 1069 936 635 543 450 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5563 Fragments arranged by size: 5563 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2501 3032 3035 3038 1186 Size: 1315 531 3 3 3936 Fragments arranged by size: 3936 1315 531 3 3 BseYI C'CCAG_C Cuts at: 2631 2965 4991 5487 2631 Size: 334 2026 496 2932 Fragments arranged by size: 2932 2026 496 334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5215 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2401 3238 3999 5027 1540 Size: 275 186 400 837 761 1028 2301 Fragments arranged by size: 2301 1028 837 761 400 275 186 BsiHKAI G_wGCw'C Cuts at: 2093 2144 2208 2931 2952 3849 3934 5005 2093 Size: 51 64 723 21 897 85 1071 2876 Fragments arranged by size: 2876 1071 897 723 85 64 51 21 BsmI GAATG_Cn' Cuts at: 2501 3299 3392 2501 Size: 798 93 4897 Fragments arranged by size: 4897 798 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2136 2144 2184 2208 2247 2256 Size: 399 303 43 8 40 24 39 9 Cuts at: 2256 2667 2752 2873 2931 2952 3107 3849 3934 Size: 411 85 121 58 21 155 742 85 Cuts at: 3934 5005 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 411 399 303 155 121 85 85 58 43 40 39 24 21 9 8 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3143 844 Size: 2299 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2866 3675 1394 Size: 429 1043 809 3507 Fragments arranged by size: 3507 1043 809 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2919 4283 4457 65 Size: 1183 1671 1364 174 1396 Fragments arranged by size: 1671 1396 1364 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3046 4432 4619 1700 Size: 110 1236 1386 187 2869 Fragments arranged by size: 2869 1386 1236 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 2195 1925 Size: 270 5518 BssSI C'ACGA_G Cuts at: 3848 4860 3848 Size: 1012 4776 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5788 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstXI CCAn_nnnn'nTGG Cuts at: 2284 2965 2284 Size: 681 5107 BstYI r'GATC_y Cuts at: 1972 2112 3118 3485 3870 3887 5339 5425 5437 Size: 140 1006 367 385 17 1452 86 12 Cuts at: 5437 5783 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1006 385 367 346 140 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2150 2214 2676 2753 3030 513 Size: 552 1085 64 462 77 277 3271 Fragments arranged by size: 3271 1085 552 462 277 77 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 2650 3304 4098 4118 2650 Size: 654 794 20 4320 Fragments arranged by size: 4320 794 654 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 2424 3092 2424 Size: 668 5120 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2082 2364 Size: 54 1425 50 275 18 225 27 282 Cuts at: 2364 2547 2886 3235 4124 5753 8 Size: 183 339 349 889 1629 43 Fragments arranged by size: 1629 1425 889 349 339 282 275 225 183 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3718 1353 Size: 2365 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2669 3065 4375 4749 4895 387 Size: 1064 138 1080 396 1310 374 146 1280 Fragments arranged by size: 1310 1280 1080 1064 396 374 146 138 EcoICRI GAG'CTC Cuts at: 2142 2142 Size: 5788 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2377 2400 2513 2804 3095 3851 4448 Size: 393 745 23 113 291 291 756 597 Cuts at: 4448 5235 5503 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 756 745 597 393 291 291 268 113 23 EcoO109I rG'GnC_Cy Cuts at: 2243 2830 2869 2870 2243 Size: 587 39 1 5161 Fragments arranged by size: 5161 587 39 1 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 2030 2079 2162 4935 1678 Size: 352 49 83 2773 2531 Fragments arranged by size: 2773 2531 352 83 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 3046 3078 4429 Size: 32 135 32 803 32 639 32 1351 Cuts at: 4429 4461 4503 4535 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1351 803 639 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KasI G'GCGC_C Cuts at: 2075 2075 Size: 5788 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2322 4902 5086 1305 Size: 1017 2580 184 2007 Fragments arranged by size: 2580 2007 1017 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 2549 10 Size: 54 1425 343 225 492 3249 Fragments arranged by size: 3249 1425 492 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2924 2960 3746 4105 4264 518 Size: 648 1758 36 786 359 159 2042 Fragments arranged by size: 2042 1758 786 648 359 159 36 MspA1I CmG'CkG Cuts at: 1606 2152 2216 2347 3073 3204 3881 5029 5274 Size: 546 64 131 726 131 677 1148 245 Cuts at: 5274 1606 Size: 2120 Fragments arranged by size: 2120 1148 726 677 546 245 131 131 64 NaeI GCC'GGC Cuts at: 3048 3048 Size: 5788 NarI GG'CG_CC Cuts at: 2076 2076 Size: 5788 NcoI C'CATG_G Cuts at: 513 1065 2676 3030 513 Size: 552 1611 354 3271 Fragments arranged by size: 3271 1611 552 354 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NgoMIV G'CCGG_C Cuts at: 3046 3046 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5788 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5788 PfoI T'CCnGG_A Cuts at: 2106 2283 2106 Size: 177 5611 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2243 2869 2243 Size: 626 5162 PstI C_TGCA'G Cuts at: 830 3140 830 Size: 2310 3478 PvuI CG_AT'CG Cuts at: 2001 2401 2001 Size: 400 5388 PvuII CAG'CTG Cuts at: 3073 3073 Size: 5788 SacI G_AGCT'C Cuts at: 2144 2144 Size: 5788 SacII CC_GC'GG Cuts at: 2153 2217 2153 Size: 64 5724 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 3136 4271 4952 5143 826 Size: 219 2091 1135 681 191 1471 Fragments arranged by size: 2091 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2077 2077 Size: 5788 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 2179 2248 2537 3115 1872 Size: 307 69 289 578 4545 Fragments arranged by size: 4545 578 307 289 69 SmlI C'TyrA_G Cuts at: 820 1017 1943 2714 3892 4793 5055 820 Size: 197 926 771 1178 901 262 1553 Fragments arranged by size: 1553 1178 926 901 771 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5788 StyI C'CwwG_G Cuts at: 513 1065 2676 2724 3030 3227 513 Size: 552 1611 48 306 197 3074 Fragments arranged by size: 3074 1611 552 306 197 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3815 3832 3985 4170 4615 325 Size: 1255 2235 17 153 185 445 1498 Fragments arranged by size: 2235 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4034 5746 96 Size: 274 80 33 51 1443 2057 1712 138 Fragments arranged by size: 2057 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5748 5780 5748 Size: 32 5756 Tth111I GACn'n_nGTC Cuts at: 1316 2974 1316 Size: 1658 4130 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 2177 2246 2535 3113 1870 Size: 307 69 289 578 4545 Fragments arranged by size: 4545 578 307 289 69 XmnI GAAnn'nnTTC Cuts at: 2501 3917 2501 Size: 1416 4372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2527 3596 276 Size: 53 83 186 936 993 1069 2468 Fragments arranged by size: 2468 1069 993 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI