(Linear) (Six-base) MAPSORT of: KIBB7616.seq Check: 9811 from: 1 to: 1083 KIBB7616.orf With 182 enzymes: SgfI * March 22, 2012 15:38 .. AatII G_ACGT'C Cuts at: 0 524 1083 Size: 524 559 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 395 1083 Size: 395 688 AfeI AGC'GCT Cuts at: 0 23 155 1083 Size: 23 132 928 Fragments arranged by size: 928 132 23 AleI CACnn'nnGTG Cuts at: 0 955 1083 Size: 955 128 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 937 973 1083 Size: 937 36 110 Fragments arranged by size: 937 110 36 AlwNI CAG_nnn'CTG Cuts at: 0 828 1083 Size: 828 255 ApaI G_GGCC'C Cuts at: 0 242 868 1083 Size: 242 626 215 Fragments arranged by size: 626 242 215 ApaLI G'TGCA_C Cuts at: 0 84 1083 Size: 84 999 AvaI C'yCGr_G Cuts at: 0 63 132 172 241 530 697 808 1083 Size: 63 69 40 69 289 167 111 275 Fragments arranged by size: 289 275 167 111 69 69 63 40 BanI G'GyrC_C Cuts at: 0 70 1083 Size: 70 1013 BanII G_rGCy'C Cuts at: 0 131 139 242 251 662 868 1083 Size: 131 8 103 9 411 206 215 Fragments arranged by size: 411 215 206 131 103 9 8 BbeI G_GCGC'C Cuts at: 0 74 1083 Size: 74 1009 BbsI GAAGACnn'nnnn_ Cuts at: 0 572 1040 1083 Size: 572 468 43 Fragments arranged by size: 572 468 43 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 75 109 776 810 1083 Size: 75 34 667 34 273 Fragments arranged by size: 667 273 75 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 566 1083 Size: 566 517 BglI GCCn_nnn'nGGC Cuts at: 0 246 1083 Size: 246 837 BlpI GC'TnA_GC Cuts at: 0 25 202 1083 Size: 25 177 881 Fragments arranged by size: 881 177 25 Bme1580I G_kGCm'C Cuts at: 0 88 179 242 747 868 1083 Size: 88 91 63 505 121 215 Fragments arranged by size: 505 215 121 91 88 63 BmrI ACTGGGnnnn_n' Cuts at: 0 98 766 1083 Size: 98 668 317 Fragments arranged by size: 668 317 98 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 372 508 799 1083 Size: 372 136 291 284 Fragments arranged by size: 372 291 284 136 Bpu10I CC'TnA_GC Cuts at: 0 875 938 1083 Size: 875 63 145 Fragments arranged by size: 875 145 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 694 1083 Size: 694 389 BsaHI Gr'CG_yC Cuts at: 0 71 521 1083 Size: 71 450 562 Fragments arranged by size: 562 450 71 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 496 1027 1030 1033 1083 Size: 496 531 3 3 50 Fragments arranged by size: 531 496 50 3 3 BseYI C'CCAG_C Cuts at: 0 626 960 1083 Size: 626 334 123 Fragments arranged by size: 626 334 123 BsiEI CG_ry'CG Cuts at: 0 396 1083 Size: 396 687 BsiHKAI G_wGCw'C Cuts at: 0 88 139 203 926 947 1083 Size: 88 51 64 723 21 136 Fragments arranged by size: 723 136 88 64 51 21 BsmI GAATG_Cn' Cuts at: 0 496 1083 Size: 496 587 Bsp1286I G_dGCh'C Cuts at: 0 88 131 139 179 203 242 251 662 Size: 88 43 8 40 24 39 9 411 Cuts at: 662 747 868 926 947 1083 Size: 85 121 58 21 136 Fragments arranged by size: 411 136 121 88 85 58 43 40 39 24 21 9 8 BsrBI CCG'CTC Cuts at: 0 861 1083 Size: 861 222 BsrDI GCAATG_nn' Cuts at: 0 914 1083 Size: 914 169 BsrFI r'CCGG_y Cuts at: 0 1041 1083 Size: 1041 42 BssHII G'CGCG_C Cuts at: 0 190 1083 Size: 190 893 BstXI CCAn_nnnn'nTGG Cuts at: 0 279 960 1083 Size: 279 681 123 Fragments arranged by size: 681 279 123 BstYI r'GATC_y Cuts at: 0 107 1083 Size: 107 976 BtgI C'CryG_G Cuts at: 0 145 209 671 748 1025 1083 Size: 145 64 462 77 277 58 Fragments arranged by size: 462 277 145 77 64 58 BtsI GCAGTG_nn' Cuts at: 0 645 1083 Size: 645 438 DraI TTT'AAA Cuts at: 0 419 1083 Size: 419 664 EaeI y'GGCC_r Cuts at: 0 50 77 359 542 881 1083 Size: 50 27 282 183 339 202 Fragments arranged by size: 339 282 202 183 50 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 664 1060 1083 Size: 664 396 23 Fragments arranged by size: 664 396 23 EcoICRI GAG'CTC Cuts at: 0 137 1083 Size: 137 946 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 372 395 508 799 1083 Size: 372 23 113 291 284 Fragments arranged by size: 372 291 284 113 23 EcoO109I rG'GnC_Cy Cuts at: 0 238 825 864 865 1083 Size: 238 587 39 1 218 Fragments arranged by size: 587 238 218 39 1 HaeII r_GCGC'y Cuts at: 0 25 74 157 1083 Size: 25 49 83 926 Fragments arranged by size: 926 83 49 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 370 402 1041 1073 1083 Size: 370 32 639 32 10 Fragments arranged by size: 639 370 32 32 10 KasI G'GCGC_C Cuts at: 0 70 1083 Size: 70 1013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 317 1083 Size: 317 766 MscI TGG'CCA Cuts at: 0 52 544 1083 Size: 52 492 539 Fragments arranged by size: 539 492 52 MslI CAynn'nnrTG Cuts at: 0 919 955 1083 Size: 919 36 128 Fragments arranged by size: 919 128 36 MspA1I CmG'CkG Cuts at: 0 147 211 342 1068 1083 Size: 147 64 131 726 15 Fragments arranged by size: 726 147 131 64 15 NaeI GCC'GGC Cuts at: 0 1043 1083 Size: 1043 40 NarI GG'CG_CC Cuts at: 0 71 1083 Size: 71 1012 NcoI C'CATG_G Cuts at: 0 671 1025 1083 Size: 671 354 58 Fragments arranged by size: 671 354 58 NgoMIV G'CCGG_C Cuts at: 0 1041 1083 Size: 1041 42 PfoI T'CCnGG_A Cuts at: 0 101 278 1083 Size: 101 177 805 Fragments arranged by size: 805 177 101 PspOMI G'GGCC_C Cuts at: 0 238 864 1083 Size: 238 626 219 Fragments arranged by size: 626 238 219 PvuI CG_AT'CG Cuts at: 0 396 1083 Size: 396 687 PvuII CAG'CTG Cuts at: 0 1068 1083 Size: 1068 15 SacI G_AGCT'C Cuts at: 0 139 1083 Size: 139 944 SacII CC_GC'GG Cuts at: 0 148 212 1083 Size: 148 64 871 Fragments arranged by size: 871 148 64 SfoI GGC'GCC Cuts at: 0 72 1083 Size: 72 1011 SmaI CCC'GGG Cuts at: 0 174 243 532 1083 Size: 174 69 289 551 Fragments arranged by size: 551 289 174 69 SmlI C'TyrA_G Cuts at: 0 709 1083 Size: 709 374 StyI C'CwwG_G Cuts at: 0 671 719 1025 1083 Size: 671 48 306 58 Fragments arranged by size: 671 306 58 48 Tth111I GACn'n_nGTC Cuts at: 0 969 1083 Size: 969 114 XmaI C'CCGG_G Cuts at: 0 172 241 530 1083 Size: 172 69 289 553 Fragments arranged by size: 553 289 172 69 XmnI GAAnn'nnTTC Cuts at: 0 496 1083 Size: 496 587 ZraI GAC'GTC Cuts at: 0 522 1083 Size: 522 561 Enzymes that do cut: AatII AcuI AfeI AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI BciVI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI BssHII BstXI BstYI BtgI BtsI DraI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PfoI PspOMI PvuI PvuII SacI SacII SfoI SmaI SmlI StyI Tth111I XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AflIII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BclI BfrBI BglII BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmBI BspEI BspHI BspMI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI RsrII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI XbaI XcmI XhoI