Restriction map for
pFN21AE2490
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AccII AciI AclI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII BbeI BbrPI BcgI BclI
BcnI BfrI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DsaI EagI
Eam1105I EarI EclXI Eco52I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 474 GGTACC G^GTACC
AccIII 803 TCCGGA T^CCGGA
AlwNI 1264 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1109 CCTCAGG CC^T[ACGT]AGG
Aor51HI 55 AGCGCT AGC^GCT
Asp718I 474 GGTACC G^GTACC
AspHI 168 GAGCAC G[AT]GC[AT]^C
BamHI 505 GGATCC G^GATCC
BanI 474 GGTACC G^G[CT][GA]CC
BbsI 105 GTCTTC GAAGAC(2/6)
BbvCI 921 GCTGAGG CCTCAGC(-5/-2)
BciVI 40 GTATCC GTATCC(6/5)
BglI 1237 GCCTTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 449 AGATCT A^GATCT
BpmI 772 CTGGAG CTGGAG(16/14)
BpuAI 105 GTCTTC GAAGAC(2/6)
BsaI 304 GAGACC GGTCTC(1/5)
BsaWI 803 TCCGGA [AT]^CCGG[AT]
BseAI 803 TCCGGA T^CCGGA
BseRI 955 GAGGAG GAGGAG(10/8)
BsgI 202 GTGCAG GTGCAG(16/14)
BsiHKAI 168 GAGCAC G[AT]GC[AT]^C
BsmBI 1225 GAGACG CGTCTC(1/5)
BspEI 803 TCCGGA T^CCGGA
BstAPI 204 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 864 CCAGTATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1109 CCTCAGG CC^T[ACGT]AGG
Cac8I 133 GCCAGC GC[ACGT]^[ACGT]GC
DraI 1047 TTTAAA TTT^AAA
DraII 586 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1183 GACAGCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 55 AGCGCT AGC^GCT
Eco81I 1109 CCTCAGG CC^T[ACGT]AGG
EcoNI 1155 CCTGTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 586 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 182 GAATTC G^AATTC
HapII 804 CCGG C^CGG
HincII 1009 GTTGAC GT[CT]^[GA]AC
HindII 1009 GTTGAC GT[CT]^[GA]AC
HpaII 804 CCGG C^CGG
KpnI 474 GGTACC GGTAC^C
MaeII 622 ACGT A^CGT
MroI 803 TCCGGA T^CCGGA
MspA1I 556 CAGCTG C[AC]G^C[GT]G
MspI 804 CCGG C^CGG
PflMI 5 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 337 GACTC GAGTC(4/5)
PpuMI 586 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 556 CAGCTG CAG^CTG
SfiI 1236 GGCCTTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 180 TCGA T^CGA
TfiI 250 GATTC G^A[AT]TC
TthHB8I 180 TCGA T^CGA
Van91I 5 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 231 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 182 GAATTC [GA]^AATT[CT]
540 GAATTT
AfaI 475 GTAC GT^AC
931 GTAC
ApoI 182 GAATTC [GA]^AATT[CT]
540 GAATTT
AvrII 597 CCTAGG C^CTAGG
661 CCTAGG
BlnI 597 CCTAGG C^CTAGG
661 CCTAGG
BsmFI 586 GGGAC GGGAC(10/14)
784 GGGAC
BstF5I 501 GGATG GGATG(2/0)
1164 GGATG
CfoI 56 GCGC GCG^C
131 GCGC
FokI 501 GGATG GGATG(9/13)
1164 GGATG
HaeII 55 AGCGCT [GA]GCGC^[CT]
130 AGCGCC
HhaI 56 GCGC GCG^C
131 GCGC
HinP1I 56 GCGC G^CGC
131 GCGC
HinfI 250 GATTC G^A[ACGT]TC
337 GACTC
RsaI 475 GTAC GT^AC
931 GTAC
Tsp45I 353 GTCAC ^GT[GC]AC
462 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AluI AlwI AvaII BalI BanII
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI BstYI Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI HaeIII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI Tru9I TseI Tsp509I
TspRI XhoII