(Circular) (Six-base) MAPSORT of: pFN21AE2490.seq Check: 3691 from: 1 to: 5989 pFN21AE2490 5989 bp With 182 enzymes: SgfI * November 15, 2013 16:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3799 278 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3332 1937 Size: 1395 4594 Acc65I G'GTAC_C Cuts at: 2479 3310 2479 Size: 831 5158 AclI AA'CG_TT Cuts at: 1563 4116 4489 1563 Size: 2553 373 3063 Fragments arranged by size: 3063 2553 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2443 4052 5436 5704 1632 Size: 811 1609 1384 268 1917 Fragments arranged by size: 1917 1609 1384 811 268 AfeI AGC'GCT Cuts at: 2062 2062 Size: 5989 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5792 AflIII A'CryG_T Cuts at: 1203 4888 5865 1203 Size: 3685 977 1327 Fragments arranged by size: 3685 1327 977 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 2204 1166 Size: 1038 4951 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5989 AlwNI CAG_nnn'CTG Cuts at: 3274 3732 5304 3274 Size: 458 1572 3959 Fragments arranged by size: 3959 1572 458 ApaLI G'TGCA_C Cuts at: 4046 5202 4046 Size: 1156 4833 ApoI r'AATT_y Cuts at: 1493 2187 2545 3298 3524 3676 5785 1493 Size: 694 358 753 226 152 2109 1697 Fragments arranged by size: 2109 1697 753 694 358 226 152 AseI AT'TA_AT Cuts at: 160 1032 4543 160 Size: 872 3511 1606 Fragments arranged by size: 3511 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 3314 1870 Size: 73 1371 4545 Fragments arranged by size: 4545 1371 73 AvrII C'CTAG_G Cuts at: 2602 2666 2602 Size: 64 5925 BamHI G'GATC_C Cuts at: 2510 3319 3686 2510 Size: 809 367 4813 Fragments arranged by size: 4813 809 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2479 3310 4765 618 Size: 325 217 627 692 831 1455 1842 Fragments arranged by size: 1842 1455 831 692 627 325 217 BanII G_rGCy'C Cuts at: 1391 2325 2776 2910 3308 1391 Size: 934 451 134 398 4072 Fragments arranged by size: 4072 934 451 398 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2103 928 Size: 971 204 4814 Fragments arranged by size: 4814 971 204 BbvCI CC'TCA_GC Cuts at: 2927 2927 Size: 5989 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4180 4214 1903 Size: 34 2243 34 3678 Fragments arranged by size: 3678 2243 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2056 3881 5091 2056 Size: 1825 1210 2954 Fragments arranged by size: 2954 1825 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3248 4600 136 Size: 107 122 71 631 605 1576 1352 1525 Fragments arranged by size: 1576 1525 1352 631 605 122 107 71 BglII A'GATC_T Cuts at: 2454 5984 2454 Size: 3530 2459 BlpI GC'TnA_GC Cuts at: 3406 3406 Size: 5989 Bme1580I G_kGCm'C Cuts at: 1790 2024 2123 2802 4050 5206 1790 Size: 234 99 679 1248 1156 2573 Fragments arranged by size: 2573 1248 1156 679 234 99 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5989 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2369 2632 3720 Size: 416 53 83 399 24 952 263 1088 Cuts at: 3720 4678 442 Size: 958 1753 Fragments arranged by size: 1753 1088 958 952 416 399 263 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2798 4649 1239 Size: 1559 1851 2579 Fragments arranged by size: 2579 1851 1559 Bpu10I CC'TnA_GC Cuts at: 2295 2927 2295 Size: 632 5357 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4114 4979 5277 4114 Size: 865 298 4826 Fragments arranged by size: 4826 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2303 4652 882 Size: 617 804 2349 2219 Fragments arranged by size: 2349 2219 804 617 BsaAI yAC'GTr Cuts at: 493 1204 3780 493 Size: 711 2576 2702 Fragments arranged by size: 2702 2576 711 BsaBI GATnn'nnATC Cuts at: 5983 5983 Size: 5989 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3796 4178 4813 275 Size: 53 83 186 936 2263 382 635 1451 Fragments arranged by size: 2263 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2808 4422 4820 5094 5241 5652 2808 Size: 1614 398 274 147 411 3145 Fragments arranged by size: 3145 1614 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2803 2833 1178 Size: 30 165 30 1400 30 4334 Fragments arranged by size: 4334 1400 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2975 1186 Size: 1789 4200 BseYI C'CCAG_C Cuts at: 3237 5192 5688 3237 Size: 1955 496 3538 Fragments arranged by size: 3538 1955 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 1163 Size: 573 492 4924 Fragments arranged by size: 4924 573 492 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3439 4200 5228 1540 Size: 275 186 1438 761 1028 2301 Fragments arranged by size: 2301 1438 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2177 4050 4135 5206 2177 Size: 1873 85 1071 2960 Fragments arranged by size: 2960 1873 1071 85 BsmI GAATG_Cn' Cuts at: 3500 3593 3500 Size: 93 5896 BsmBI CGTCTCn'nnnn_ Cuts at: 3224 3224 Size: 5989 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2123 2177 2325 2776 2802 2910 Size: 399 234 99 54 148 451 26 108 Cuts at: 2910 3308 4050 4135 5206 1391 Size: 398 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 451 399 398 234 148 108 99 85 54 26 BspEI T'CCGG_A Cuts at: 2808 5652 2808 Size: 2844 3145 BspHI T'CATG_A Cuts at: 3878 5608 3878 Size: 1730 4259 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3344 844 Size: 2500 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3876 1394 Size: 429 2053 3507 Fragments arranged by size: 3507 2053 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4484 4658 65 Size: 1183 3236 174 1396 Fragments arranged by size: 3236 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4633 4820 1700 Size: 110 2823 187 2869 Fragments arranged by size: 2869 2823 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5989 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5989 BssSI C'ACGA_G Cuts at: 4049 5061 4049 Size: 1012 4977 BstAPI GCAn_nnn'nTGC Cuts at: 794 2215 794 Size: 1421 4568 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5989 BstXI CCAn_nnnn'nTGG Cuts at: 2876 2876 Size: 5989 BstYI r'GATC_y Cuts at: 1972 2454 2510 2847 3139 3319 3686 4071 4088 Size: 482 56 337 292 180 367 385 17 Cuts at: 4088 5540 5626 5638 5984 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 482 385 367 346 337 292 180 86 56 17 12 Bsu36I CC'TnA_GG Cuts at: 3115 3115 Size: 5989 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4498 Fragments arranged by size: 4498 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5250 Fragments arranged by size: 5250 683 56 BtsI GCAGTG_nn' Cuts at: 2497 3505 4299 4319 2497 Size: 1008 794 20 4167 Fragments arranged by size: 4167 1008 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5989 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5954 DraI TTT'AAA Cuts at: 3054 3293 3054 Size: 239 5750 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3194 4996 809 Size: 507 1878 1802 1802 Fragments arranged by size: 1878 1802 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2334 2569 Size: 54 1425 50 275 18 177 327 235 Cuts at: 2569 3436 4325 5954 8 Size: 867 889 1629 43 Fragments arranged by size: 1629 1425 889 867 327 275 235 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3436 1537 Size: 275 1624 4090 Fragments arranged by size: 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3919 1353 Size: 2566 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4576 4950 5096 387 Size: 1064 138 2987 374 146 1280 Fragments arranged by size: 2987 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2443 2798 4052 4649 5436 5704 1239 Size: 393 811 355 1254 597 787 268 1524 Fragments arranged by size: 1524 1254 811 787 597 393 355 268 EcoNI CCTnn'n_nnAGG Cuts at: 3164 3164 Size: 5989 EcoO109I rG'GnC_Cy Cuts at: 2592 2592 Size: 5989 EcoRI G'AATT_C Cuts at: 2187 3298 3676 2187 Size: 1111 378 4500 Fragments arranged by size: 4500 1111 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2473 3190 2473 Size: 717 5272 FspI TGC'GCA Cuts at: 4495 4495 Size: 5989 HaeII r_GCGC'y Cuts at: 1678 2064 2139 5136 1678 Size: 386 75 2997 2531 Fragments arranged by size: 2997 2531 386 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4630 4662 4704 4736 1373 Size: 32 135 32 3058 32 42 32 2626 Fragments arranged by size: 3058 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3016 3333 3579 669 Size: 988 281 1078 317 246 3079 Fragments arranged by size: 3079 1078 988 317 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5989 HpaI GTT'AAC Cuts at: 3579 3579 Size: 5989 KpnI G_GTAC'C Cuts at: 2483 3314 2483 Size: 831 5158 MfeI C'AATT_G Cuts at: 3588 3588 Size: 5989 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2538 2814 5103 5287 1305 Size: 1233 276 2289 184 2007 Fragments arranged by size: 2289 2007 1233 276 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2336 2571 10 Size: 54 1425 343 177 327 235 3428 Fragments arranged by size: 3428 1425 343 327 235 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2204 3023 3947 4306 4465 518 Size: 648 873 165 819 924 359 159 2042 Fragments arranged by size: 2042 924 873 819 648 359 165 159 MspA1I CmG'CkG Cuts at: 1606 2563 3405 4082 5230 5475 1606 Size: 957 842 677 1148 245 2120 Fragments arranged by size: 2120 1148 957 842 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4498 Fragments arranged by size: 4498 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5989 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5989 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 3347 4892 1132 Size: 97 2118 1545 2229 Fragments arranged by size: 2229 2118 1545 97 PasI CC'CwG_GG Cuts at: 1348 2767 1348 Size: 1419 4570 PciI A'CATG_T Cuts at: 4888 4888 Size: 5989 PflMI CCAn_nnn'nTGG Cuts at: 1108 2016 1108 Size: 908 5081 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4058 4090 5571 5603 4058 Size: 32 1481 32 4444 Fragments arranged by size: 4444 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2592 2592 Size: 5989 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5989 PsiI TTA'TAA Cuts at: 2709 3559 2709 Size: 850 5139 PstI C_TGCA'G Cuts at: 830 3341 830 Size: 2511 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 PvuII CAG'CTG Cuts at: 2563 2563 Size: 5989 SalI G'TCGA_C Cuts at: 1936 3331 1936 Size: 1395 4594 SbfI CC_TGCA'GG Cuts at: 3341 3341 Size: 5989 ScaI AGT'ACT Cuts at: 4237 5949 4237 Size: 1712 4277 SfcI C'TryA_G Cuts at: 826 1045 3337 4472 5153 5344 826 Size: 219 2292 1135 681 191 1471 Fragments arranged by size: 2292 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3248 3248 Size: 5989 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 3316 1872 Size: 1444 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4093 4994 5256 820 Size: 197 926 2150 901 262 1553 Fragments arranged by size: 2150 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5989 SphI G_CATG'C Cuts at: 1132 3347 1132 Size: 2215 3774 SspI AAT'ATT Cuts at: 5 52 3913 5 Size: 47 3861 2081 Fragments arranged by size: 3861 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5989 StyI C'CwwG_G Cuts at: 513 1065 2004 2427 2595 2602 2666 3243 3428 Size: 552 939 423 168 7 64 577 185 Cuts at: 3428 513 Size: 3074 Fragments arranged by size: 3074 939 577 552 423 185 168 64 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2633 3021 4016 4033 4186 4371 4816 Size: 1255 1053 388 995 17 153 185 445 Cuts at: 4816 325 Size: 1498 Fragments arranged by size: 1498 1255 1053 995 445 388 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4235 5947 96 Size: 274 80 33 51 1443 2258 1712 138 Fragments arranged by size: 2258 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2828 2860 5949 5981 2828 Size: 32 3089 32 2836 Fragments arranged by size: 3089 2836 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5989 XbaI T'CTAG_A Cuts at: 2236 3325 2236 Size: 1089 4900 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 3314 1870 Size: 1444 4545 XmnI GAAnn'nnTTC Cuts at: 4118 4118 Size: 5989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3797 276 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI