Restriction map for
pFN21AE1934
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfaI BlnI BsaAI BsaBI
BsaI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 659 GTCGAC GT^[AC][GT]AC
AccIII 782 TCCGGA T^CCGGA
AcyI 1270 GACGCC G[GA]^CG[CT]C
AflII 1114 CTTAAG C^TTAAG
AocI 1086 CCTCAGG CC^T[ACGT]AGG
AspHI 1279 GAGCAC G[AT]GC[AT]^C
BamHI 10 GGATCC G^GATCC
BanI 322 GGCACC G^G[CT][GA]CC
BcgI 933 CGATGACGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1114 CTTAAG C^TTAAG
BglI 1391 GCCTCACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1110 AGATCT A^GATCT
BlpI 907 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 907 GCTGAGC GC^T[ACGT]AGC
BsaHI 1270 GACGCC G[GA]^CG[CT]C
BseAI 782 TCCGGA T^CCGGA
BseRI 49 GAGGAG GAGGAG(10/8)
BsgI 196 GTGCAG GTGCAG(16/14)
BsiHKAI 1279 GAGCAC G[AT]GC[AT]^C
BspEI 782 TCCGGA T^CCGGA
BstBI 360 TTCGAA TT^CGAA
Bsu36I 1086 CCTCAGG CC^T[ACGT]AGG
CelII 907 GCTGAGC GC^T[ACGT]AGC
CfoI 1389 GCGC GCG^C
DrdI 708 GACTGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1086 CCTCAGG CC^T[ACGT]AGG
EcoRV 646 GATATC GAT^ATC
EcoT14I 922 CCTTGG C^C[AT][AT]GG
HaeII 1388 AGCGCC [GA]GCGC^[CT]
HgaI 1270 GACGC GACGC(5/10)
HhaI 1389 GCGC GCG^C
Hin1I 1270 GACGCC G[GA]^CG[CT]C
HinP1I 1389 GCGC G^CGC
HincII 659 GTCGAC GT[CT]^[GA]AC
HindII 659 GTCGAC GT[CT]^[GA]AC
HphI 305 TCACC GGTGA(8/7)
MroI 782 TCCGGA T^CCGGA
MspA1I 905 CAGCTG C[AC]G^C[GT]G
NspI 738 GCATGT [GA]CATG^[CT]
NspV 360 TTCGAA TT^CGAA
PflMI 917 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 227 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 905 CAGCTG CAG^CTG
SalI 659 GTCGAC G^TCGAC
SexAI 1028 ACCAGGT A^CC[AT]GGT
SfcI 1129 CTACAG C^T[GA][CT]AG
SfuI 360 TTCGAA TT^CGAA
StuI 610 AGGCCT AGG^CCT
StyI 922 CCTTGG C^C[AT][AT]GG
Van91I 917 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 913 CCATCCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 182 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
1089 CAGGTTCTG
BalI 1139 TGGCCA TGG^CCA
1355 TGGCCA
BbsI 88 GAAGAC GAAGAC(2/6)
1267 GAAGAC
BbvI 265 GCTGC GCAGC(8/12)
1350 GCTGC
BcnI 14 CCGGG CC^[GC]GG
732 CCCGG
BpuAI 88 GAAGAC GAAGAC(2/6)
1267 GAAGAC
BstYI 10 GGATCC [GA]^GATC[CT]
1110 AGATCT
DraII 227 AGGACCC [GA]G^G[ACGT]CC[CT]
481 AGGGCCT
EaeI 1139 TGGCCA [CT]^GGCC[GA]
1355 TGGCCA
EarI 712 GAAGAG CTCTTC(1/4)
1299 GAAGAG
EcoNI 485 CCTTTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
613 CCTGTGCCAGG
EcoO109I 227 AGGACCC [GA]G^G[ACGT]CC[CT]
481 AGGGCCT
EcoRI 502 GAATTC G^AATTC
1069 GAATTC
Fnu4HI 265 GCTGC GC^[ACGT]GC
1350 GCTGC
ItaI 265 GCTGC GC^[ACGT]GC
1350 GCTGC
Ksp632I 712 GAAGAG CTCTTC(1/4)
1299 GAAGAG
MaeII 663 ACGT A^CGT
938 ACGT
MflI 10 GGATCC [GA]^GATC[CT]
1110 AGATCT
MluNI 1139 TGGCCA TGG^CCA
1355 TGGCCA
MscI 1139 TGGCCA TGG^CCA
1355 TGGCCA
NciI 14 CCGGG CC^[GC]GG
732 CCCGG
SfaNI 105 GATGC GCATC(5/9)
1404 GATGC
SmlI 5 CTCAAG C^T[CT][GA]AG
1114 CTTAAG
TseI 265 GCTGC G^C[AT]GC
1350 GCTGC
XhoII 10 GGATCC [GA]^GATC[CT]
1110 AGATCT
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BmyI BpmI
BsaJI BsaWI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FauI FokI HaeIII HapII HinfI
HpaII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI TthHB8I