Restriction map for
pFN21AE6355
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AciI
AclI AcyI AflII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MfeI
MroI MseI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 15 CGCG CG^CG
AcsI 925 AAATTC [GA]^AATT[CT]
AfaI 414 GTAC GT^AC
AflIII 14 ACGCGT A^C[GA][CT]GT
AhdI 752 GACGCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 925 AAATTC [GA]^AATT[CT]
AspEI 752 GACGCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 995 GAGCTC G[AT]GC[AT]^C
BanII 995 GAGCTC G[GA]GC[CT]^C
BbvCI 351 CCTCAGC CCTCAGC(-5/-2)
BclI 575 TGATCA T^GATCA
BsaBI 573 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 995 GAGCTC G[AT]GC[AT]^C
BsmAI 769 GAGAC GTCTC(1/5)
Bsp1407I 413 TGTACA T^GTACA
BspMI 370 ACCTGC ACCTGC(4/8)
BsrDI 900 CATTGC GCAATG(2/0)
BsrGI 413 TGTACA T^GTACA
BstUI 15 CGCG CG^CG
BstYI 431 GGATCT [GA]^GATC[CT]
DraII 443 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 134 CCATGG C^C[GA][CT]GG
Eam1105I 752 GACGCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 443 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 480 GATATC GAT^ATC
FbaI 575 TGATCA T^GATCA
HaeII 59 AGCGCC [GA]GCGC^[CT]
HapII 249 CCGG C^CGG
HinfI 933 GACTC G^A[ACGT]TC
HpaII 249 CCGG C^CGG
MaeIII 604 GTCAC ^GT[ACGT]AC
MamI 573 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 431 GGATCT [GA]^GATC[CT]
MluI 14 ACGCGT A^CGCGT
MspA1I 965 CAGCTG C[AC]G^C[GT]G
MspI 249 CCGG C^CGG
MvnI 15 CGCG CG^CG
NcoI 134 CCATGG C^CATGG
NspI 548 ACATGC [GA]CATG^[CT]
PflMI 905 CCATATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 933 GACTC GAGTC(4/5)
PpuMI 443 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 965 CAGCTG CAG^CTG
RsaI 414 GTAC GT^AC
SacI 995 GAGCTC GAGCT^C
SapI 633 GAAGAGC GCTCTTC(1/4)
SspBI 413 TGTACA T^GTACA
Tsp45I 604 GTCAC ^GT[GC]AC
Van91I 905 CCATATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 431 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 431 GGATC GGATC(4/5)
518 GGATC
Asp700I 886 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
982 GAAACAATTC
BanI 459 GGTGCC G^G[CT][GA]CC
1009 GGCACC
BbsI 244 GTCTTC GAAGAC(2/6)
319 GTCTTC
BglI 171 GCCTAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
190 GCCCTGGTGGC
BpmI 423 CTCCAG CTGGAG(16/14)
299 CTGGAG
BpuAI 244 GTCTTC GAAGAC(2/6)
319 GTCTTC
BsmFI 341 GGGAC GGGAC(10/14)
443 GGGAC
Cac8I 56 GCAAGC GC[ACGT]^[ACGT]GC
180 GCTGGC
CfoI 60 GCGC GCG^C
552 GCGC
DdeI 79 CTGAG C^T[ACGT]AG
352 CTCAG
EarI 727 CTCTTC CTCTTC(1/4)
633 GAAGAG
HhaI 60 GCGC GCG^C
552 GCGC
HinP1I 60 GCGC G^CGC
552 GCGC
HphI 35 TCACC GGTGA(8/7)
295 TCACC
Ksp632I 727 CTCTTC CTCTTC(1/4)
633 GAAGAG
Tsp509I 926 AATT ^AATT
987 AATT
XcmI 185 CCATCGCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
803 CCATCCTCAATGTGG
XmnI 886 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
982 GAAACAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AluI AvaII BalI BbvI BmyI
BsaJI BseRI BsgI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cfr13I DpnII EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HgaI
ItaI MboI MboII MluNI MnlI
MscI MslI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI