(Circular) (Six-base) MAPSORT of: pFN21AE6355.seq Check: 870 from: 1 to: 5752 pFN21AE6355 5752 bp With 182 enzymes: SgfI * July 14, 2018 22:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2384 2384 Size: 5752 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3095 1937 Size: 1158 4594 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 2311 2474 2853 3003 3815 5199 5467 Size: 647 32 163 379 150 812 1384 268 Cuts at: 5467 1632 Size: 1917 Fragments arranged by size: 1917 1384 812 647 379 268 163 150 32 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 2019 4651 5628 1203 Size: 816 2632 977 1327 Fragments arranged by size: 2632 1327 977 816 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 2762 4481 2762 Size: 1719 4033 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AlwNI CAG_nnn'CTG Cuts at: 3495 5067 3495 Size: 1572 4180 ApaLI G'TGCA_C Cuts at: 3809 4965 3809 Size: 1156 4596 ApoI r'AATT_y Cuts at: 1493 2930 3061 3287 3439 5548 1493 Size: 1437 131 226 152 2109 1697 Fragments arranged by size: 2109 1697 1437 226 152 131 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 3077 1870 Size: 73 1134 4545 Fragments arranged by size: 4545 1134 73 BamHI G'GATC_C Cuts at: 3082 3449 3082 Size: 367 5385 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2464 3014 3073 4528 618 Size: 325 217 627 677 550 59 1455 1842 Fragments arranged by size: 1842 1455 677 627 550 325 217 59 BanII G_rGCy'C Cuts at: 1391 3004 3071 1391 Size: 1613 67 4072 Fragments arranged by size: 4072 1613 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2242 2317 928 Size: 971 343 75 4363 Fragments arranged by size: 4363 971 343 75 BbvCI CC'TCA_GC Cuts at: 2357 2357 Size: 5752 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3943 3977 1903 Size: 34 2006 34 3678 Fragments arranged by size: 3678 2006 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 2580 1548 Size: 1032 4720 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2182 2201 Size: 107 122 71 631 605 337 173 19 Cuts at: 2201 4363 136 Size: 2162 1525 Fragments arranged by size: 2162 1525 631 605 337 173 122 107 71 19 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 2395 2469 3813 4969 1790 Size: 605 74 1344 1156 2573 Fragments arranged by size: 2573 1344 1156 605 74 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3483 4441 442 Size: 416 53 83 399 24 2066 958 1753 Fragments arranged by size: 2066 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3052 3052 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2325 2413 4412 1239 Size: 1086 88 1999 2579 Fragments arranged by size: 2579 1999 1086 88 Bpu10I CC'TnA_GC Cuts at: 2357 2357 Size: 5752 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3877 4742 5040 3877 Size: 865 298 4589 Fragments arranged by size: 4589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4415 882 Size: 617 2916 2219 Fragments arranged by size: 2916 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3543 493 Size: 711 2339 2702 Fragments arranged by size: 2702 2339 711 BsaBI GATnn'nnATC Cuts at: 2582 5746 2582 Size: 3164 2588 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3559 3941 4576 275 Size: 53 83 186 936 2026 382 635 1451 Fragments arranged by size: 2026 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4185 4583 4857 5004 5415 4185 Size: 398 274 147 411 4522 Fragments arranged by size: 4522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2411 2441 1178 Size: 30 165 30 1008 30 4489 Fragments arranged by size: 4489 1008 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2402 2671 2855 2891 1186 Size: 1216 269 184 36 4047 Fragments arranged by size: 4047 1216 269 184 36 BseYI C'CCAG_C Cuts at: 2128 2239 2710 4955 5451 2128 Size: 111 471 2245 496 2429 Fragments arranged by size: 2429 2245 496 471 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2119 2617 2773 1163 Size: 573 383 498 156 4142 Fragments arranged by size: 4142 573 498 383 156 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3202 3963 4991 1540 Size: 275 186 1201 761 1028 2301 Fragments arranged by size: 2301 1201 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3004 3813 3898 4969 3004 Size: 809 85 1071 3787 Fragments arranged by size: 3787 1071 809 85 BsmI GAATG_Cn' Cuts at: 3263 3356 3263 Size: 93 5659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2395 2469 3004 3071 3813 3898 4969 Size: 399 605 74 535 67 742 85 1071 Cuts at: 4969 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 605 535 399 85 74 67 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 3641 5371 3641 Size: 1730 4022 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2384 3107 844 Size: 1540 723 3489 Fragments arranged by size: 3489 1540 723 BsrBI CCG'CTC Cuts at: 1394 1823 3639 1394 Size: 429 1816 3507 Fragments arranged by size: 3507 1816 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2904 4247 4421 65 Size: 1183 1656 1343 174 1396 Fragments arranged by size: 1656 1396 1343 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4396 4583 1700 Size: 110 2586 187 2869 Fragments arranged by size: 2869 2586 187 110 BsrGI T'GTAC_A Cuts at: 96 2418 96 Size: 2322 3430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 3812 4824 3812 Size: 1012 4740 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5752 BstBI TT'CG_AA Cuts at: 4556 4556 Size: 5752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2197 2632 2077 Size: 120 435 5197 Fragments arranged by size: 5197 435 120 BstYI r'GATC_y Cuts at: 1972 2436 3082 3449 3834 3851 5303 5389 5401 Size: 464 646 367 385 17 1452 86 12 Cuts at: 5401 5747 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 646 464 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2139 513 Size: 552 939 135 4126 Fragments arranged by size: 4126 939 552 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2176 487 Size: 683 56 950 4063 Fragments arranged by size: 4063 950 683 56 BtsI GCAGTG_nn' Cuts at: 2605 3268 4062 4082 2605 Size: 663 794 20 4275 Fragments arranged by size: 4275 794 663 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5717 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2277 2334 Size: 54 1425 50 275 18 357 90 57 Cuts at: 2334 2543 2604 3199 4088 5717 8 Size: 209 61 595 889 1629 43 Fragments arranged by size: 1629 1425 889 595 357 275 209 90 61 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 1537 Size: 275 1387 4090 Fragments arranged by size: 4090 1387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2633 2738 3682 1353 Size: 1280 105 944 3423 Fragments arranged by size: 3423 1280 944 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3002 3002 Size: 5752 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2311 2325 2413 2474 2853 3003 Size: 393 647 32 14 88 61 379 150 Cuts at: 3003 3815 4412 5199 5467 1239 Size: 812 597 787 268 1524 Fragments arranged by size: 1524 812 787 647 597 393 379 268 150 88 61 32 14 EcoO109I rG'GnC_Cy Cuts at: 2449 2449 Size: 5752 EcoRI G'AATT_C Cuts at: 3061 3439 3061 Size: 378 5374 EcoRV GAT'ATC Cuts at: 2487 2487 Size: 5752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2182 2182 Size: 5752 FspI TGC'GCA Cuts at: 4258 4258 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 2068 4899 1678 Size: 390 2831 2531 Fragments arranged by size: 2831 2531 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2146 2178 2565 2597 2748 Size: 32 135 32 574 32 387 32 151 Cuts at: 2748 2780 4393 4425 4467 4499 1373 Size: 32 1613 32 42 32 2626 Fragments arranged by size: 2626 1613 574 387 151 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3096 3342 669 Size: 988 281 1158 246 3079 Fragments arranged by size: 3079 1158 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5752 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 3351 3351 Size: 5752 MluI A'CGCG_T Cuts at: 2019 5628 2019 Size: 3609 2143 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2818 4866 5050 1305 Size: 1513 2048 184 2007 Fragments arranged by size: 2048 2007 1513 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2189 2279 2336 10 Size: 54 1425 343 357 90 57 3426 Fragments arranged by size: 3426 1425 357 343 90 57 54 MslI CAynn'nnrTG Cuts at: 518 1166 2096 2366 2630 3710 4069 4228 518 Size: 648 930 270 264 1080 359 159 2042 Fragments arranged by size: 2042 1080 930 648 359 270 264 159 MspA1I CmG'CkG Cuts at: 1606 2972 3168 3845 4993 5238 1606 Size: 1366 196 677 1148 245 2120 Fragments arranged by size: 2120 1366 1148 677 245 196 NcoI C'CATG_G Cuts at: 513 1065 2004 2139 513 Size: 552 939 135 4126 Fragments arranged by size: 4126 939 552 135 NdeI CA'TA_TG Cuts at: 387 387 Size: 5752 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 2557 3110 4655 1132 Size: 97 1328 553 1545 2229 Fragments arranged by size: 2229 1545 1328 553 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5752 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 2916 1108 Size: 1808 3944 PfoI T'CCnGG_A Cuts at: 2995 2995 Size: 5752 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2449 2449 Size: 5752 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5752 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PstI C_TGCA'G Cuts at: 830 3104 830 Size: 2274 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 PvuII CAG'CTG Cuts at: 2972 2972 Size: 5752 SacI G_AGCT'C Cuts at: 3004 3004 Size: 5752 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SapI GCTCTTCn'nnn_ Cuts at: 2633 2633 Size: 5752 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SfcI C'TryA_G Cuts at: 826 1045 3100 4235 4916 5107 826 Size: 219 2055 1135 681 191 1471 Fragments arranged by size: 2055 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 3079 1872 Size: 1207 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3856 4757 5019 820 Size: 197 926 1913 901 262 1553 Fragments arranged by size: 1913 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 3110 1132 Size: 1978 3774 SspI AAT'ATT Cuts at: 5 52 3676 5 Size: 47 3624 2081 Fragments arranged by size: 3624 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5752 StyI C'CwwG_G Cuts at: 513 1065 2004 2139 2395 2943 3191 513 Size: 552 939 135 256 548 248 3074 Fragments arranged by size: 3074 939 552 548 256 248 135 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2065 3779 3796 3949 4134 4579 325 Size: 1255 485 1714 17 153 185 445 1498 Fragments arranged by size: 1714 1498 1255 485 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 3998 5710 Size: 274 80 33 51 1443 441 1580 1712 Cuts at: 5710 96 Size: 138 Fragments arranged by size: 1712 1580 1443 441 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5712 5744 5712 Size: 32 5720 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5752 XbaI T'CTAG_A Cuts at: 3088 3088 Size: 5752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 2815 2197 Size: 618 5134 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 3077 1870 Size: 1207 4545 XmnI GAAnn'nnTTC Cuts at: 2895 2991 3881 2895 Size: 96 890 4766 Fragments arranged by size: 4766 890 96 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI