Restriction map for
pFN21AE6355
(Linear) (Six-base) MAPSORT of: KIEE6355.seq Check: 1993 from: 1 to: 1047
KIEE6355.orf
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 379 1047
Size: 379 668
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 306 469 848 998 1047
Size: 274 32 163 379 150 49
Fragments arranged by size:
379 274 163 150 49 32
AflIII A'CryG_T
Cuts at: 0 14 1047
Size: 14 1033
AhdI GACnn_n'nnGTC
Cuts at: 0 757 1047
Size: 757 290
ApoI r'AATT_y
Cuts at: 0 925 1047
Size: 925 122
BanI G'GyrC_C
Cuts at: 0 459 1009 1047
Size: 459 550 38
Fragments arranged by size:
550 459 38
BanII G_rGCy'C
Cuts at: 0 999 1047
Size: 999 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 237 312 1047
Size: 237 75 735
Fragments arranged by size:
735 237 75
BbvCI CC'TCA_GC
Cuts at: 0 352 1047
Size: 352 695
BclI T'GATC_A
Cuts at: 0 575 1047
Size: 575 472
BglI GCCn_nnn'nGGC
Cuts at: 0 177 196 1047
Size: 177 19 851
Fragments arranged by size:
851 177 19
Bme1580I G_kGCm'C
Cuts at: 0 390 464 1047
Size: 390 74 583
Fragments arranged by size:
583 390 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1047
Size: 1047
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 408 1047
Size: 320 88 639
Fragments arranged by size:
639 320 88
Bpu10I CC'TnA_GC
Cuts at: 0 352 1047
Size: 352 695
BsaBI GATnn'nnATC
Cuts at: 0 577 1047
Size: 577 470
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 406 436 1047
Size: 406 30 611
Fragments arranged by size:
611 406 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 397 666 850 886 1047
Size: 397 269 184 36 161
Fragments arranged by size:
397 269 184 161 36
BseYI C'CCAG_C
Cuts at: 0 123 234 705 1047
Size: 123 111 471 342
Fragments arranged by size:
471 342 123 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 612 768 1047
Size: 114 498 156 279
Fragments arranged by size:
498 279 156 114
BsiHKAI G_wGCw'C
Cuts at: 0 999 1047
Size: 999 48
Bsp1286I G_dGCh'C
Cuts at: 0 390 464 999 1047
Size: 390 74 535 48
Fragments arranged by size:
535 390 74 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 379 1047
Size: 379 668
BsrDI GCAATG_nn'
Cuts at: 0 899 1047
Size: 899 148
BsrGI T'GTAC_A
Cuts at: 0 413 1047
Size: 413 634
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 192 627 1047
Size: 72 120 435 420
Fragments arranged by size:
435 420 120 72
BstYI r'GATC_y
Cuts at: 0 431 1047
Size: 431 616
BtgI C'CryG_G
Cuts at: 0 134 1047
Size: 134 913
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 171 1047
Size: 171 876
BtsI GCAGTG_nn'
Cuts at: 0 600 1047
Size: 600 447
EaeI y'GGCC_r
Cuts at: 0 182 272 329 538 599 1047
Size: 182 90 57 209 61 448
Fragments arranged by size:
448 209 182 90 61 57
EarI CTCTTCn'nnn_
Cuts at: 0 628 733 1047
Size: 628 105 314
Fragments arranged by size:
628 314 105
EcoICRI GAG'CTC
Cuts at: 0 997 1047
Size: 997 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 306 320 408 469 848 998 1047
Size: 274 32 14 88 61 379 150 49
Fragments arranged by size:
379 274 150 88 61 49 32 14
EcoO109I rG'GnC_Cy
Cuts at: 0 444 1047
Size: 444 603
EcoRV GAT'ATC
Cuts at: 0 482 1047
Size: 482 565
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 177 1047
Size: 177 870
HaeII r_GCGC'y
Cuts at: 0 63 1047
Size: 63 984
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 560 592 743 775 1047
Size: 141 32 387 32 151 32 272
Fragments arranged by size:
387 272 151 141 32 32 32
MluI A'CGCG_T
Cuts at: 0 14 1047
Size: 14 1033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 1047
Size: 813 234
MscI TGG'CCA
Cuts at: 0 184 274 331 1047
Size: 184 90 57 716
Fragments arranged by size:
716 184 90 57
MslI CAynn'nnrTG
Cuts at: 0 91 361 625 1047
Size: 91 270 264 422
Fragments arranged by size:
422 270 264 91
MspA1I CmG'CkG
Cuts at: 0 967 1047
Size: 967 80
NcoI C'CATG_G
Cuts at: 0 134 1047
Size: 134 913
NspI r_CATG'y
Cuts at: 0 552 1047
Size: 552 495
PflMI CCAn_nnn'nTGG
Cuts at: 0 911 1047
Size: 911 136
PfoI T'CCnGG_A
Cuts at: 0 990 1047
Size: 990 57
PpuMI rG'GwC_Cy
Cuts at: 0 444 1047
Size: 444 603
PvuII CAG'CTG
Cuts at: 0 967 1047
Size: 967 80
SacI G_AGCT'C
Cuts at: 0 999 1047
Size: 999 48
SapI GCTCTTCn'nnn_
Cuts at: 0 628 1047
Size: 628 419
StyI C'CwwG_G
Cuts at: 0 134 390 938 1047
Size: 134 256 548 109
Fragments arranged by size:
548 256 134 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 60 1047
Size: 60 987
TatI w'GTAC_w
Cuts at: 0 413 1047
Size: 413 634
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 192 810 1047
Size: 192 618 237
Fragments arranged by size:
618 237 192
XmnI GAAnn'nnTTC
Cuts at: 0 890 986 1047
Size: 890 96 61
Fragments arranged by size:
890 96 61
Enzymes that do cut:
AarI AcuI AflIII AhdI ApoI BanI BanII BbsI
BbvCI BclI BglI Bme1580I BplI BpmI Bpu10I BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BsrGI BstXI BstYI BtgI BtgZI BtsI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I MluI
MmeI MscI MslI MspA1I NcoI NspI PflMI PfoI
PpuMI PvuII SacI SapI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI
BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI