Restriction map for
pFN21AE6264
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 780 ACATGT A^C[GA][CT]GT
AlwI 403 GATCC GGATC(4/5)
AocI 790 CCTGAGG CC^T[ACGT]AGG
Asp700I 190 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 286 GAGCAC G[AT]GC[AT]^C
AvaII 366 GGACC G^G[AT]CC
BalI 734 TGGCCA TGG^CCA
BanI 558 GGCACC G^G[CT][GA]CC
BbvCI 846 CCTCAGC CCTCAGC(-5/-2)
BcnI 752 CCGGG CC^[GC]GG
BfaI 422 CTAG C^TAG
BsaAI 55 TACGTG [CT]AC^GT[GA]
BsiHKAI 286 GAGCAC G[AT]GC[AT]^C
BsmAI 30 GTCTC GTCTC(1/5)
BsmFI 754 GGGAC GGGAC(10/14)
BsmI 390 GCATTC GAATGC(1/-1)
BspLU11I 780 ACATGT A^CATGT
BsrBI 607 GAGCGG CCGCTC(-3/-3)
BstAPI 559 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 790 CCTGAGG CC^T[ACGT]AGG
Cfr13I 366 GGACC G^G[ACGT]CC
DpnII 403 GATC ^GATC
Eco81I 790 CCTGAGG CC^T[ACGT]AGG
EcoT22I 592 ATGCAT ATGCA^T
MaeI 422 CTAG C^TAG
MaeII 56 ACGT A^CGT
MboI 403 GATC ^GATC
MboII 190 GAAGA GAAGA(8/7)
MluNI 734 TGGCCA TGG^CCA
MscI 734 TGGCCA TGG^CCA
NciI 752 CCGGG CC^[GC]GG
NcoI 665 CCATGG C^CATGG
NdeII 403 GATC ^GATC
NlaIV 558 GGCACC GG[ACGT]^[ACGT]CC
NsiI 592 ATGCAT ATGCA^T
PshAI 513 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 467 CTGCAG CTGCA^G
Sau3AI 403 GATC ^GATC
Sau96I 366 GGACC G^G[ACGT]CC
SfcI 467 CTGCAG C^T[GA][CT]AG
SmlI 199 CTTGAG C^T[CT][GA]AG
StuI 843 AGGCCT AGG^CCT
TaqI 615 TCGA T^CGA
TspRI 516 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 615 TCGA T^CGA
XcmI 443 CCACGCTGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 190 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 609 GCGG CCGC(-3/-1)
800 GCGG
AfaI 175 GTAC GT^AC
778 GTAC
BmyI 286 GAGCAC G[AGT]GC[ACT]^C
808 GGGCAC
Bsp1286I 286 GAGCAC G[AGT]GC[ACT]^C
808 GGGCAC
BstNI 855 CCTGG CC^[AT]GG
870 CCTGG
BstXI 440 CCACCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
725 CCACGGGAGTGG
EaeI 59 TGGCCG [CT]^GGCC[GA]
734 TGGCCA
EcoNI 708 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
786 CCTCCCTGAGG
EcoRII 855 CCTGG ^CC[AT]GG
870 CCTGG
EcoT14I 74 CCTTGG C^C[AT][AT]GG
665 CCATGG
HapII 62 CCGG C^CGG
752 CCGG
HpaII 62 CCGG C^CGG
752 CCGG
MseI 83 TTAA T^TAA
526 TTAA
MslI 277 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
726 CACGGGAGTG
MspI 62 CCGG C^CGG
752 CCGG
MvaI 855 CCTGG CC^[AT]GG
870 CCTGG
RsaI 175 GTAC GT^AC
778 GTAC
SphI 312 GCATGC GCATG^C
594 GCATGC
StyI 74 CCTTGG C^C[AT][AT]GG
665 CCATGG
Tru9I 83 TTAA T^TAA
526 TTAA
Tsp45I 112 GTGAC ^GT[GC]AC
670 GTGAC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsrI BssKI BstF5I Cac8I DdeI
DsaI Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MnlI MspA1I
MwoI NlaIII NspI PvuII ScrFI
SfaNI TfiI TseI Tsp509I