Restriction map for
pFN21AE6264
(Circular) (Six-base) MAPSORT of: pFN21AE6264.seq Check: 3639 from: 1 to: 5626
pFN21AE6264 5626 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 2193 3689 5073 5341 1632
Size: 497 64 1496 1384 268 1917
Fragments arranged by size:
1917 1496 1384 497 268 64
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 2785 4525 5502 1203
Size: 1582 1740 977 1327
Fragments arranged by size:
1740 1582 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 2735 1166
Size: 1569 4057
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5626
AlwNI CAG_nnn'CTG
Cuts at: 3369 4941 3369
Size: 1572 4054
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2935 3161 3313 5422 1493
Size: 1442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 1870
Size: 73 1008 4545
Fragments arranged by size:
4545 1008 73
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2563 2947 4402 618
Size: 325 217 627 776 384 1455 1842
Fragments arranged by size:
1842 1455 776 627 384 325 217
BanII G_rGCy'C
Cuts at: 1391 2945 1391
Size: 1554 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4655
BbvCI CC'TCA_GC
Cuts at: 2852 2852
Size: 5626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3817 3851 1903
Size: 34 1880 34 3678
Fragments arranged by size:
3678 1880 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BfrBI ATG'CAT
Cuts at: 2599 2599
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4237 136
Size: 107 122 71 631 605 2565 1525
Fragments arranged by size:
2565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5621 5621
Size: 5626
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 2817 3687 4843 1790
Size: 1027 870 1156 2573
Fragments arranged by size:
2573 1156 1027 870
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4315 442
Size: 416 53 83 399 24 1940 958 1753
Fragments arranged by size:
1940 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4286 1239
Size: 3047 2579
Bpu10I CC'TnA_GC
Cuts at: 2852 2852
Size: 5626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 3751 4616 4914 2225
Size: 1526 865 298 2937
Fragments arranged by size:
2937 1526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 882
Size: 617 2790 2219
Fragments arranged by size:
2790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2062 3417 493
Size: 711 858 1355 2702
Fragments arranged by size:
2702 1355 858 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3433 3815 4450 275
Size: 53 83 186 936 1900 382 635 1451
Fragments arranged by size:
1900 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2775 2805 1178
Size: 30 165 30 1372 30 3999
Fragments arranged by size:
3999 1372 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5626
BseYI C'CCAG_C
Cuts at: 2452 4829 5325 2452
Size: 2377 496 2753
Fragments arranged by size:
2753 2377 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5053
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3076 3837 4865 1540
Size: 275 186 1075 761 1028 2301
Fragments arranged by size:
2301 1075 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2295 3687 3772 4843 2295
Size: 1392 85 1071 3078
Fragments arranged by size:
3078 1392 1071 85
BsmI GAATG_Cn'
Cuts at: 2395 3137 3230 2395
Size: 742 93 4791
Fragments arranged by size:
4791 742 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2295 2817 2945 3687 3772 4843 1391
Size: 399 505 522 128 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 522 505 399 128 85
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 3515 5245 3515
Size: 1730 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2981 844
Size: 2137 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2614 3513 1394
Size: 429 791 899 3507
Fragments arranged by size:
3507 899 791 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4121 4295 65
Size: 1183 2873 174 1396
Fragments arranged by size:
2873 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4270 4457 1700
Size: 110 2460 187 2869
Fragments arranged by size:
2869 2460 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2570 794
Size: 1776 3850
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstXI CCAn_nnnn'nTGG
Cuts at: 2452 2737 2452
Size: 285 5341
BstYI r'GATC_y
Cuts at: 1972 2956 3323 3708 3725 5177 5263 5275 5621
Size: 984 367 385 17 1452 86 12 346
Cuts at: 5621 1972
Size: 1977
Fragments arranged by size:
1977 1452 984 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2796 2796
Size: 5626
BtgI C'CryG_G
Cuts at: 513 1065 2004 2670 2730 2835 513
Size: 552 939 666 60 105 3304
Fragments arranged by size:
3304 939 666 552 105 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 3142 3936 3956 3142
Size: 794 20 4812
Fragments arranged by size:
4812 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2739 3073
Size: 54 1425 50 275 18 234 675 334
Cuts at: 3073 3962 5591 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 675 334 275 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3073 1537
Size: 275 1261 4090
Fragments arranged by size:
4090 1261 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3556 1353
Size: 2203 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2820 4213 4587 4733 387
Size: 1064 138 1231 1393 374 146 1280
Fragments arranged by size:
1393 1280 1231 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2193 3689 4286 5073 5341 1239
Size: 393 497 64 1496 597 787 268 1524
Fragments arranged by size:
1524 1496 787 597 497 393 268 64
EcoNI CCTnn'n_nnAGG
Cuts at: 2717 2795 2717
Size: 78 5548
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2219 2729 2219
Size: 510 5116
FspI TGC'GCA
Cuts at: 4132 4132
Size: 5626
HaeII r_GCGC'y
Cuts at: 1678 4773 1678
Size: 3095 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 4267 4299 4341
Size: 32 135 32 431 32 2232 32 42
Cuts at: 4341 4373 1373
Size: 32 2626
Fragments arranged by size:
2626 2232 431 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4740 4924 1305
Size: 3435 184 2007
Fragments arranged by size:
3435 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2741 10
Size: 54 1425 343 909 2895
Fragments arranged by size:
2895 1425 909 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2286 2735 2922 3584 3943 4102 518
Size: 648 1120 449 187 662 359 159 2042
Fragments arranged by size:
2042 1120 662 648 449 359 187 159
MspA1I CmG'CkG
Cuts at: 1606 2314 2477 2570 2856 3042 3719 4867 5112
Size: 708 163 93 286 186 677 1148 245
Cuts at: 5112 1606
Size: 2120
Fragments arranged by size:
2120 1148 708 677 286 245 186 163
93
NcoI C'CATG_G
Cuts at: 513 1065 2004 2670 513
Size: 552 939 666 3469
Fragments arranged by size:
3469 939 666 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NsiI A_TGCA'T
Cuts at: 2601 2601
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2186 2321 2603 2789 2927 2984 4529
Size: 97 957 135 282 186 138 57 1545
Cuts at: 4529 1132
Size: 2229
Fragments arranged by size:
2229 1545 957 282 186 138 135 97
57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5626
PciI A'CATG_T
Cuts at: 2785 4525 2785
Size: 1740 3886
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5626
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2522 1613
Size: 909 4717
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PstI C_TGCA'G
Cuts at: 830 2476 2978 830
Size: 1646 502 3478
Fragments arranged by size:
3478 1646 502
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
PvuII CAG'CTG
Cuts at: 2314 2477 2570 2856 2314
Size: 163 93 286 5084
Fragments arranged by size:
5084 286 163 93
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2472 2974 4109 4790 4981 826
Size: 219 1427 502 1135 681 191 1471
Fragments arranged by size:
1471 1427 1135 681 502 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2204 3730 4631 4893 820
Size: 197 926 261 1526 901 262 1553
Fragments arranged by size:
1553 1526 926 901 262 261 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2321 2603 2984 1132
Size: 1189 282 381 3774
Fragments arranged by size:
3774 1189 381 282
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 2850 1517
Size: 1333 4293
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 2670 3065 513
Size: 552 939 75 591 395 3074
Fragments arranged by size:
3074 939 591 552 395 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 3653 3670 3823 4008 4453 325
Size: 1255 537 1536 17 153 185 445 1498
Fragments arranged by size:
1536 1498 1255 537 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2179 2782 3872
Size: 274 80 33 51 1443 202 603 1090
Cuts at: 3872 5584 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1090 603 274 202 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2046 2078 5586 5618 2046
Size: 32 3508 32 2054
Fragments arranged by size:
3508 2054 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2455 2455
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 2199 3755 2199
Size: 1556 4070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI