################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BcgI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI KspI MaeII MamI MfeI MflI MluI MroI MslI MunI MvnI NaeI NcoI NdeI NgoMIV NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Alw44I 682 GTGCAC G^TGCAC Aor51HI 575 AGCGCT AGC^GCT ApaI 878 GGGCCC GGGCC^C ApaLI 682 GTGCAC G^TGCAC AvaI 191 CCCGAG C^[CT]CG[GA]G BbeI 136 GGCGCC GGCGC^C BciVI 255 GGATAC GTATCC(6/5) BcnI 662 CCGGG CC^[GC]GG BlpI 898 GCTGAGC GC^T[ACGT]AGC Bpu1102I 898 GCTGAGC GC^T[ACGT]AGC BsaWI 938 TCCGGT [AT]^CCGG[AT] BsmBI 160 GAGACG CGTCTC(1/5) BsmFI 15 GGGAC GGGAC(10/14) BsoBI 191 CCCGAG C^[CT]CG[GA]G BspMI 467 ACCTGC ACCTGC(4/8) BsrBI 699 CCGCTC CCGCTC(-3/-3) BssSI 632 CTCGTG CACGAG(-5/-1) BstF5I 59 CATCC GGATG(2/0) CelII 898 GCTGAGC GC^T[ACGT]AGC DrdI 861 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 748 CTCTTC CTCTTC(1/4) Eco47III 575 AGCGCT AGC^GCT EcoNI 807 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 376 GCGGG CCCGC(4/6) FokI 59 CATCC GGATG(9/13) HgaI 692 GCGTC GACGC(5/10) HincII 436 GTCAAC GT[CT]^[GA]AC HindII 436 GTCAAC GT[CT]^[GA]AC KasI 136 GGCGCC G^GCGCC Ksp632I 748 CTCTTC CTCTTC(1/4) MseI 512 TTAA T^TAA NarI 136 GGCGCC GG^CGCC NciI 662 CCGGG CC^[GC]GG NheI 178 GCTAGC G^CTAGC PflMI 270 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 859 AGGACCT [GA]G^G[AT]CC[CT] PstI 207 CTGCAG CTGCA^G SacI 172 GAGCTC GAGCT^C SapI 747 GCTCTTC GCTCTTC(1/4) ScaI 839 AGTACT AGT^ACT SfoI 136 GGCGCC GGC^GCC Tru9I 512 TTAA T^TAA Tsp509I 287 AATT ^AATT Van91I 270 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 136 GGCGCC G[GA]^CG[CT]C 691 GGCGTC AfaI 217 GTAC GT^AC 840 GTAC AlwNI 271 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 846 CAGTGACTG AspHI 172 GAGCTC G[AT]GC[AT]^C 682 GTGCAC BalI 614 TGGCCA TGG^CCA 641 TGGCCA BanI 136 GGCGCC G^G[CT][GA]CC 658 GGCACC BbvCI 325 GCTGAGG CCTCAGC(-5/-2) 811 GCTGAGG BclI 128 TGATCA T^GATCA 365 TGATCA BfaI 179 CTAG C^TAG 931 CTAG BsaHI 136 GGCGCC G[GA]^CG[CT]C 691 GGCGTC BsiHKAI 172 GAGCTC G[AT]GC[AT]^C 682 GTGCAC BsmAI 160 GAGAC GTCTC(1/5) 870 GTCTC BsrI 133 ACTGG ACTGG(1/-1) 239 CCAGT BstEII 6 GGTCACC G^GT[ACGT]ACC 666 GGTCACC BstPI 6 GGTCACC G^GT[ACGT]ACC 666 GGTCACC DpnII 129 GATC ^GATC 366 GATC EcoO65I 6 GGTCACC G^GT[ACGT]ACC 666 GGTCACC FbaI 128 TGATCA T^GATCA 365 TGATCA Hin1I 136 GGCGCC G[GA]^CG[CT]C 691 GGCGTC MaeI 179 CTAG C^TAG 931 CTAG MboI 129 GATC ^GATC 366 GATC MboII 32 TCTTC GAAGA(8/7) 749 TCTTC MluNI 614 TGGCCA TGG^CCA 641 TGGCCA MscI 614 TGGCCA TGG^CCA 641 TGGCCA NdeII 129 GATC ^GATC 366 GATC RsaI 217 GTAC GT^AC 840 GTAC Sau3AI 129 GATC ^GATC 366 GATC SfcI 207 CTGCAG C^T[GA][CT]AG 579 CTACAG TfiI 546 GATTC G^A[AT]TC 935 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MnlI MspA1I MspI MvaI MwoI NlaIV PleI PvuII Sau96I ScrFI StyI TseI Tsp45I TspRI