(Circular) (Six-base) MAPSORT of: pFN21AE6633.seq Check: 7796 from: 1 to: 5653 pFN21AE6633 5653 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 1937 Size: 1059 4594 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2246 2833 3716 5100 5368 1632 Size: 614 587 883 1384 268 1917 Fragments arranged by size: 1917 1384 883 614 587 268 AfeI AGC'GCT Cuts at: 2582 2582 Size: 5653 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 2281 2856 3396 4968 2281 Size: 575 540 1572 2966 Fragments arranged by size: 2966 1572 575 540 ApaI G_GGCC'C Cuts at: 2887 2887 Size: 5653 ApaLI G'TGCA_C Cuts at: 2687 3710 4866 2687 Size: 1023 1156 3474 Fragments arranged by size: 3474 1156 1023 ApoI r'AATT_y Cuts at: 1493 2962 3188 3340 5449 1493 Size: 1469 226 152 2109 1697 Fragments arranged by size: 2109 1697 1469 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2196 2978 1870 Size: 73 253 782 4545 Fragments arranged by size: 4545 782 253 73 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 2663 2974 4429 618 Size: 325 217 627 354 522 311 1455 1842 Fragments arranged by size: 1842 1455 627 522 354 325 311 217 BanII G_rGCy'C Cuts at: 1391 2181 2197 2832 2887 2972 1391 Size: 790 16 635 55 85 4072 Fragments arranged by size: 4072 790 635 85 55 16 BbeI G_GCGC'C Cuts at: 2145 2145 Size: 5653 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4682 BbvCI CC'TCA_GC Cuts at: 2331 2817 2331 Size: 486 5167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3844 3878 1903 Size: 34 50 34 1823 34 3678 Fragments arranged by size: 3678 1823 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2254 3545 4755 2254 Size: 1291 1210 3152 Fragments arranged by size: 3152 1291 1210 BclI T'GATC_A Cuts at: 1548 2133 2370 1548 Size: 585 237 4831 Fragments arranged by size: 4831 585 237 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4264 136 Size: 107 122 71 631 605 2592 1525 Fragments arranged by size: 2592 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 2904 3070 2904 Size: 166 5487 Bme1580I G_kGCm'C Cuts at: 1790 2691 2887 3714 4870 1790 Size: 901 196 827 1156 2573 Fragments arranged by size: 2573 1156 901 827 196 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3384 4342 442 Size: 416 53 83 399 24 1967 958 1753 Fragments arranged by size: 1967 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2187 1056 Size: 1131 4522 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2552 2846 4313 1239 Size: 1262 51 294 1467 2579 Fragments arranged by size: 2579 1467 1262 294 51 Bpu10I CC'TnA_GC Cuts at: 2331 2817 2331 Size: 486 5167 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3444 493 Size: 711 2240 2702 Fragments arranged by size: 2702 2240 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 2697 3460 3842 Size: 53 83 186 936 609 555 763 382 Cuts at: 3842 4477 275 Size: 635 1451 Fragments arranged by size: 1451 936 763 635 609 555 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2943 4086 4484 4758 4905 5316 2943 Size: 1143 398 274 147 411 3280 Fragments arranged by size: 3280 1143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2184 2214 1178 Size: 30 165 30 781 30 4617 Fragments arranged by size: 4617 781 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2205 2453 2716 1186 Size: 1019 248 263 4123 Fragments arranged by size: 4123 1019 263 248 BseYI C'CCAG_C Cuts at: 4856 5352 4856 Size: 496 5157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2129 2490 2786 1163 Size: 573 393 361 296 4030 Fragments arranged by size: 4030 573 393 361 296 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 2691 3714 3799 4870 2181 Size: 510 1023 85 1071 2964 Fragments arranged by size: 2964 1071 1023 510 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 BsmBI CGTCTCn'nnnn_ Cuts at: 2159 2159 Size: 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2197 2691 2832 2887 2972 3714 Size: 399 391 16 494 141 55 85 742 Cuts at: 3714 3799 4870 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 494 399 391 141 85 85 55 16 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2481 3008 844 Size: 1637 527 3489 Fragments arranged by size: 3489 1637 527 BsrBI CCG'CTC Cuts at: 1394 1823 2706 3540 1394 Size: 429 883 834 3507 Fragments arranged by size: 3507 883 834 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 4322 65 Size: 1183 2900 174 1396 Fragments arranged by size: 2900 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 2637 3713 4725 2637 Size: 1076 1012 3565 Fragments arranged by size: 3565 1076 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 2011 2671 1184 Size: 827 660 4166 Fragments arranged by size: 4166 827 660 BstYI r'GATC_y Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648 Size: 1011 367 385 17 1452 86 12 346 Cuts at: 5648 1972 Size: 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 2691 2803 3169 3963 3983 2691 Size: 112 366 794 20 4361 Fragments arranged by size: 4361 794 366 112 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2872 4660 809 Size: 507 1556 1788 1802 Fragments arranged by size: 1802 1788 1556 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2169 2619 2646 Size: 54 1425 50 275 18 339 450 27 Cuts at: 2646 3100 3989 5618 8 Size: 454 889 1629 43 Fragments arranged by size: 1629 1425 889 454 450 339 275 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2759 3583 1353 Size: 1406 824 3423 Fragments arranged by size: 3423 1406 824 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2179 2179 Size: 5653 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2501 2552 2833 2846 3716 4313 Size: 393 614 255 51 281 13 870 597 Cuts at: 4313 5100 5368 1239 Size: 787 268 1524 Fragments arranged by size: 1524 870 787 614 597 393 281 268 255 51 13 EcoNI CCTnn'n_nnAGG Cuts at: 2816 2816 Size: 5653 EcoO109I rG'GnC_Cy Cuts at: 2821 2865 2883 2821 Size: 44 18 5591 Fragments arranged by size: 5591 44 18 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2572 2572 Size: 5653 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 2145 2584 2911 4800 1678 Size: 467 439 327 1889 2531 Fragments arranged by size: 2531 1889 467 439 327 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2184 2216 2297 2329 4294 Size: 32 135 32 612 32 81 32 1965 Cuts at: 4294 4326 4368 4400 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1965 612 135 81 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2443 2997 3243 669 Size: 988 281 505 554 246 3079 Fragments arranged by size: 3079 988 554 505 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KasI G'GCGC_C Cuts at: 2141 2141 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2328 4767 4951 1305 Size: 1023 2439 184 2007 Fragments arranged by size: 2439 2007 1023 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2621 2648 10 Size: 54 1425 343 789 27 3015 Fragments arranged by size: 3015 1425 789 343 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 3611 3970 4129 518 Size: 648 2445 359 159 2042 Fragments arranged by size: 2445 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2414 2903 2925 3069 3746 4894 5139 1606 Size: 808 489 22 144 677 1148 245 2120 Fragments arranged by size: 2120 1148 808 677 489 245 144 22 NarI GG'CG_CC Cuts at: 2142 2142 Size: 5653 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5101 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 2183 1052 Size: 1131 4522 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5653 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 2281 1108 Size: 1173 4480 PfoI T'CCnGG_A Cuts at: 2478 2478 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2865 2865 Size: 5653 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PspOMI G'GGCC_C Cuts at: 2883 2883 Size: 5653 PstI C_TGCA'G Cuts at: 830 2216 3005 830 Size: 1386 789 3478 Fragments arranged by size: 3478 1386 789 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2414 2903 2925 2414 Size: 489 22 5142 Fragments arranged by size: 5142 489 22 SacI G_AGCT'C Cuts at: 2181 2181 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SapI GCTCTTCn'nnn_ Cuts at: 2759 2759 Size: 5653 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 2846 3901 5613 2846 Size: 1055 1712 2886 Fragments arranged by size: 2886 1712 1055 SfcI C'TryA_G Cuts at: 826 1045 2212 2584 3001 4136 4817 5008 826 Size: 219 1167 372 417 1135 681 191 1471 Fragments arranged by size: 1471 1167 1135 681 417 372 219 191 SfoI GGC'GCC Cuts at: 2143 2143 Size: 5653 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3757 4658 4920 820 Size: 197 926 1814 901 262 1553 Fragments arranged by size: 1814 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2016 2659 2879 3092 513 Size: 552 951 643 220 213 3074 Fragments arranged by size: 3074 951 643 552 220 213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2706 2860 3680 3697 3850 4035 4480 Size: 1255 1126 154 820 17 153 185 445 Cuts at: 4480 325 Size: 1498 Fragments arranged by size: 1498 1255 1126 820 445 185 154 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2221 2844 3899 Size: 274 80 33 51 1443 244 623 1055 Cuts at: 3899 5611 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1055 623 274 244 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5613 5645 5613 Size: 32 5621 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI