################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccIII AciI AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvaI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 35 CGCG CG^CG AfaI 545 GTAC GT^AC AflIII 519 ACATGT A^C[GA][CT]GT Asp700I 22 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 74 GAGCAC G[AT]GC[AT]^C BcnI 533 CCCGG CC^[GC]GG BmyI 74 GAGCAC G[AGT]GC[ACT]^C BpmI 677 CTGGAG CTGGAG(16/14) BsaJI 532 CCCCGG C^C[ACGT][ACGT]GG BsiHKAI 74 GAGCAC G[AT]GC[AT]^C BsiYI 374 CCAGAGGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 374 CCAGAGGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 157 GAGAC GTCTC(1/5) Bsp1286I 74 GAGCAC G[AGT]GC[ACT]^C BspHI 53 TCATGA T^CATGA BspLU11I 519 ACATGT A^CATGT BssKI 533 CCCGG ^CC[ACGT]GG BstUI 35 CGCG CG^CG BstXI 416 CCAAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 693 GCCTGC GC[ACGT]^[ACGT]GC CfoI 268 GCGC GCG^C DdeI 647 CTGAG C^T[ACGT]AG DraI 66 TTTAAA TTT^AAA HapII 534 CCGG C^CGG HhaI 268 GCGC GCG^C HinP1I 268 GCGC G^CGC HpaII 534 CCGG C^CGG HphI 229 GGTGA GGTGA(8/7) MaeII 543 ACGT A^CGT MslI 331 CATTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 674 CAGCTG C[AC]G^C[GT]G MspI 534 CCGG C^CGG MvnI 35 CGCG CG^CG NciI 533 CCCGG CC^[GC]GG NlaIV 690 GGAGCC GG[ACGT]^[ACGT]CC NruI 34 TCGCGA TCG^CGA NspI 519 ACATGT [GA]CATG^[CT] PstI 451 CTGCAG CTGCA^G PvuII 674 CAGCTG CAG^CTG RcaI 53 TCATGA T^CATGA RsaI 545 GTAC GT^AC ScrFI 533 CCCGG CC^[ACGT]GG SfaNI 112 GATGC GCATC(5/9) Tsp45I 230 GTGAC ^GT[GC]AC TspRI 422 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 22 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 138 GATCC GGATC(4/5) 726 GGATC BbvI 673 GCAGC GCAGC(8/12) 193 GCTGC BfaI 205 CTAG C^TAG 442 CTAG BspMI 226 GCAGGT ACCTGC(4/8) 304 GCAGGT BstF5I 474 GGATG GGATG(2/0) 573 GGATG BstYI 137 AGATCC [GA]^GATC[CT] 726 GGATCT DpnII 138 GATC ^GATC 727 GATC Fnu4HI 193 GCTGC GC^[ACGT]GC 673 GCAGC FokI 474 GGATG GGATG(9/13) 573 GGATG HinfI 279 GATTC G^A[ACGT]TC 630 GATTC ItaI 193 GCTGC GC^[ACGT]GC 673 GCAGC MaeI 205 CTAG C^TAG 442 CTAG MboI 138 GATC ^GATC 727 GATC MflI 137 AGATCC [GA]^GATC[CT] 726 GGATCT NdeII 138 GATC ^GATC 727 GATC Sau3AI 138 GATC ^GATC 727 GATC TfiI 279 GATTC G^A[AT]TC 630 GATTC TseI 193 GCTGC G^C[AT]GC 673 GCAGC XhoII 137 AGATCC [GA]^GATC[CT] 726 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BsrI EarI Ksp632I MaeIII MboII MnlI MseI NlaIII SfcI TaqI Tru9I Tsp509I TthHB8I