Restriction map for
pFN21AE6261
(Circular) (Six-base) MAPSORT of: pFN21AE6261.seq Check: 3860 from: 1 to: 5446
pFN21AE6261 5446 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2222 2300 2222
Size: 78 5368
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3256 278
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2789 1937
Size: 852 4594
Acc65I G'GTAC_C
Cuts at: 2767 2767
Size: 5446
AclI AA'CG_TT
Cuts at: 1563 3573 3946 1563
Size: 2010 373 3063
Fragments arranged by size:
3063 2010 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2224 2235 2736 3509 4893 5161 1632
Size: 491 101 11 501 773 1384 268 1917
Fragments arranged by size:
1917 1384 773 501 491 268 101 11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5249
AflIII A'CryG_T
Cuts at: 1203 2524 4345 5322 1203
Size: 1321 1821 977 1327
Fragments arranged by size:
1821 1327 1321 977
AgeI A'CCGG_T
Cuts at: 4277 4277
Size: 5446
AhdI GACnn_n'nnGTC
Cuts at: 4175 4175
Size: 5446
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5446
AlwNI CAG_nnn'CTG
Cuts at: 3189 4761 3189
Size: 1572 3874
ApaLI G'TGCA_C
Cuts at: 3503 4659 3503
Size: 1156 4290
ApoI r'AATT_y
Cuts at: 1493 2755 2981 3133 5242 1493
Size: 1262 226 152 2109 1697
Fragments arranged by size:
2109 1697 1262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4000 160
Size: 872 2968 1606
Fragments arranged by size:
2968 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 1870
Size: 73 828 4545
Fragments arranged by size:
4545 828 73
BamHI G'GATC_C
Cuts at: 2776 3143 2776
Size: 367 5079
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2767 4222 618
Size: 325 217 627 980 1455 1842
Fragments arranged by size:
1842 1455 980 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2765 1391
Size: 1374 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3637 3671 1903
Size: 34 1700 34 3678
Fragments arranged by size:
3678 1700 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3338 4548 3338
Size: 1210 4236
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5446
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4057 136
Size: 107 122 71 631 605 2385 1525
Fragments arranged by size:
2385 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5441 5441
Size: 5446
BlpI GC'TnA_GC
Cuts at: 2863 2863
Size: 5446
Bme1580I G_kGCm'C
Cuts at: 1790 3507 4663 1790
Size: 1717 1156 2573
Fragments arranged by size:
2573 1717 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5446
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3177 4135 442
Size: 416 53 83 399 24 1760 958 1753
Fragments arranged by size:
1760 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2703 4106 1239
Size: 1464 1403 2579
Fragments arranged by size:
2579 1464 1403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3571 4436 4734 3571
Size: 865 298 4283
Fragments arranged by size:
4283 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4109 882
Size: 617 2610 2219
Fragments arranged by size:
2610 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3237 493
Size: 711 2033 2702
Fragments arranged by size:
2702 2033 711
BsaBI GATnn'nnATC
Cuts at: 5440 5440
Size: 5446
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3253 3635 4270 275
Size: 53 83 186 936 1720 382 635 1451
Fragments arranged by size:
1720 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3879 4277 4551 4698 5109 3879
Size: 398 274 147 411 4216
Fragments arranged by size:
4216 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5221
Fragments arranged by size:
5221 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5446
BseYI C'CCAG_C
Cuts at: 4649 5145 4649
Size: 496 4950
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4873
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2896 3657 4685 1540
Size: 275 186 895 761 1028 2301
Fragments arranged by size:
2301 1028 895 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2083 3507 3592 4663 2083
Size: 1424 85 1071 2866
Fragments arranged by size:
2866 1424 1071 85
BsmI GAATG_Cn'
Cuts at: 2957 3050 2957
Size: 93 5353
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2765 3507 3592 4663 1391
Size: 399 293 682 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 682 399 293 85
BspEI T'CCGG_A
Cuts at: 5109 5109
Size: 5446
BspHI T'CATG_A
Cuts at: 2058 3335 5065 2058
Size: 1277 1730 2439
Fragments arranged by size:
2439 1730 1277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2222 2300 2801 844
Size: 1378 78 501 3489
Fragments arranged by size:
3489 1378 501 78
BsrBI CCG'CTC
Cuts at: 1394 1823 3333 1394
Size: 429 1510 3507
Fragments arranged by size:
3507 1510 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3941 4115 65
Size: 1183 2693 174 1396
Fragments arranged by size:
2693 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4090 4277 1700
Size: 110 2280 187 2869
Fragments arranged by size:
2869 2280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5446
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5446
BssSI C'ACGA_G
Cuts at: 3506 4518 3506
Size: 1012 4434
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5446
BstBI TT'CG_AA
Cuts at: 4250 4250
Size: 5446
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5446
BstXI CCAn_nnnn'nTGG
Cuts at: 2428 2428
Size: 5446
BstYI r'GATC_y
Cuts at: 1972 2142 2731 2776 3143 3528 3545 4997 5083
Size: 170 589 45 367 385 17 1452 86
Cuts at: 5083 5095 5441 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 589 385 367 346 170 86
45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4894
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4707
Fragments arranged by size:
4707 683 56
BtsI GCAGTG_nn'
Cuts at: 2433 2962 3756 3776 2433
Size: 529 794 20 4103
Fragments arranged by size:
4103 794 529 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5446
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5411
DraI TTT'AAA
Cuts at: 2073 2750 2073
Size: 677 4769
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4453 809
Size: 507 3137 1802
Fragments arranged by size:
3137 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2893 3782 5411
Size: 54 1425 50 275 18 1063 889 1629
Cuts at: 5411 8
Size: 43
Fragments arranged by size:
1629 1425 1063 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2893 1537
Size: 275 1081 4090
Fragments arranged by size:
4090 1081 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2589 2712 3376 1353
Size: 780 456 123 664 3423
Fragments arranged by size:
3423 780 664 456 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4033 4407 4553 387
Size: 1064 138 2444 374 146 1280
Fragments arranged by size:
2444 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2224 2235 2703 2736 3509 4106
Size: 393 491 101 11 468 33 773 597
Cuts at: 4106 4893 5161 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 773 597 491 468 393 268
101 33 11
EcoRI G'AATT_C
Cuts at: 2755 3133 2755
Size: 378 5068
FspI TGC'GCA
Cuts at: 3952 3952
Size: 5446
HaeII r_GCGC'y
Cuts at: 1678 4593 1678
Size: 2915 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4087 4119 4161 4193 1373
Size: 32 135 32 2515 32 42 32 2626
Fragments arranged by size:
2626 2515 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2790 3036 669
Size: 988 281 852 246 3079
Fragments arranged by size:
3079 988 852 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5446
HpaI GTT'AAC
Cuts at: 3036 3036
Size: 5446
KpnI G_GTAC'C
Cuts at: 2771 2771
Size: 5446
MfeI C'AATT_G
Cuts at: 3045 3045
Size: 5446
MluI A'CGCG_T
Cuts at: 5322 5322
Size: 5446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2673 4560 4744 1305
Size: 1368 1887 184 2007
Fragments arranged by size:
2007 1887 1368 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3624
Fragments arranged by size:
3624 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2340 3404 3763 3922 518
Size: 648 1174 1064 359 159 2042
Fragments arranged by size:
2042 1174 1064 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2681 2862 3539 4687 4932 1606
Size: 1075 181 677 1148 245 2120
Fragments arranged by size:
2120 1148 1075 677 245 181
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4894
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5446
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5446
NotI GC'GGCC_GC
Cuts at: 2893 2893
Size: 5446
NruI TCG'CGA
Cuts at: 2041 2041
Size: 5446
NspI r_CATG'y
Cuts at: 1132 1229 2528 2804 4349 1132
Size: 97 1299 276 1545 2229
Fragments arranged by size:
2229 1545 1299 276 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5446
PciI A'CATG_T
Cuts at: 2524 4345 2524
Size: 1821 3625
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5446
PmeI GTTT'AAAC
Cuts at: 2750 2750
Size: 5446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 5028 5060 3515
Size: 32 1481 32 3901
Fragments arranged by size:
3901 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5446
PsiI TTA'TAA
Cuts at: 3016 3016
Size: 5446
PstI C_TGCA'G
Cuts at: 830 2460 2798 830
Size: 1630 338 3478
Fragments arranged by size:
3478 1630 338
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5446
PvuII CAG'CTG
Cuts at: 2681 2681
Size: 5446
SalI G'TCGA_C
Cuts at: 1936 2788 1936
Size: 852 4594
SbfI CC_TGCA'GG
Cuts at: 2798 2798
Size: 5446
ScaI AGT'ACT
Cuts at: 3694 5406 3694
Size: 1712 3734
SfcI C'TryA_G
Cuts at: 826 1045 2247 2456 2602 2794 3929 4610 4801
Size: 219 1202 209 146 192 1135 681 191
Cuts at: 4801 826
Size: 1471
Fragments arranged by size:
1471 1202 1135 681 219 209 192 191
146
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5446
SmaI CCC'GGG
Cuts at: 1872 2773 1872
Size: 901 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3550 4451 4713 820
Size: 197 926 1607 901 262 1553
Fragments arranged by size:
1607 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3237 493
Size: 2744 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5446
SphI G_CATG'C
Cuts at: 1132 2804 1132
Size: 1672 3774
SspI AAT'ATT
Cuts at: 5 52 3370 5
Size: 47 3318 2081
Fragments arranged by size:
3318 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5446
StyI C'CwwG_G
Cuts at: 513 1065 2885 513
Size: 552 1820 3074
Fragments arranged by size:
3074 1820 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2034 3473 3490 3643 3828 4273 325
Size: 1255 454 1439 17 153 185 445 1498
Fragments arranged by size:
1498 1439 1255 454 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3692 5404 96
Size: 274 80 33 51 1443 1715 1712 138
Fragments arranged by size:
1715 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5406 5438 5406
Size: 32 5414
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5446
XbaI T'CTAG_A
Cuts at: 2782 2782
Size: 5446
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5446
XmaI C'CCGG_G
Cuts at: 1870 2771 1870
Size: 901 4545
XmnI GAAnn'nnTTC
Cuts at: 2031 3575 2031
Size: 1544 3902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3254 276
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI