################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BclI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HpaI KasI KpnI KspI MaeI MfeI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 154 GTAGAC GT^[AC][GT]AC AccII 619 CGCG CG^CG AcyI 468 GGCGTC G[GA]^CG[CT]C AflIII 618 ACGCGT A^C[GA][CT]GT AlwI 479 GATCC GGATC(4/5) AlwNI 540 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 278 AGCGCT AGC^GCT ApaI 239 GGGCCC GGGCC^C BanI 361 GGTGCC G^G[CT][GA]CC BanII 239 GGGCCC G[GA]GC[CT]^C BcgI 225 GCAGTACTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 173 GGATAC GTATCC(6/5) BglII 179 AGATCT A^GATCT BmyI 239 GGGCCC G[AGT]GC[ACT]^C BsaBI 174 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 468 GGCGTC G[GA]^CG[CT]C BsgI 587 CTGCAC GTGCAG(16/14) Bsp1286I 239 GGGCCC G[AGT]GC[ACT]^C BspHI 296 TCATGA T^CATGA BstAPI 59 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 619 CGCG CG^CG BstXI 430 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 279 GCGC GCG^C EarI 526 GAAGAG CTCTTC(1/4) Eco47III 278 AGCGCT AGC^GCT FauI 237 GCGGG CCCGC(4/6) HaeII 278 AGCGCT [GA]GCGC^[CT] HhaI 279 GCGC GCG^C Hin1I 468 GGCGTC G[GA]^CG[CT]C HinP1I 279 GCGC G^CGC HincII 127 GTTGAC GT[CT]^[GA]AC HindII 127 GTTGAC GT[CT]^[GA]AC HindIII 418 AAGCTT A^AGCTT Ksp632I 526 GAAGAG CTCTTC(1/4) MaeII 314 ACGT A^CGT MaeIII 311 GTGAC ^GT[ACGT]AC MamI 174 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 618 ACGCGT A^CGCGT MslI 162 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 543 CAGCTG C[AC]G^C[GT]G MvnI 619 CGCG CG^CG PstI 191 CTGCAG CTGCA^G PvuII 543 CAGCTG CAG^CTG RcaI 296 TCATGA T^CATGA ScaI 227 AGTACT AGT^ACT SfcI 191 CTGCAG C^T[GA][CT]AG Tsp45I 311 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AluI 419 AGCT AG^CT 544 AGCT BbsI 141 GAAGAC GAAGAC(2/6) 444 GAAGAC BpuAI 141 GAAGAC GAAGAC(2/6) 444 GAAGAC BseRI 102 CTCCTC GAGGAG(10/8) 504 GAGGAG BsiYI 346 CCGGGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 365 CCTCACCGGGG BslI 346 CCGGGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 365 CCTCACCGGGG BsmFI 44 GGGAC GGGAC(10/14) 185 GGGAC BspMI 64 ACCTGC ACCTGC(4/8) 518 GCAGGT BstF5I 318 GGATG GGATG(2/0) 440 GGATG BstYI 179 AGATCT [GA]^GATC[CT] 478 AGATCC FokI 318 GGATG GGATG(9/13) 440 GGATG HaeIII 240 GGCC GG^CC 389 GGCC HgaI 469 GCGTC GACGC(5/10) 620 GCGTC HinfI 46 GACTC G^A[ACGT]TC 609 GAGTC HphI 83 TCACC GGTGA(8/7) 367 TCACC MflI 179 AGATCT [GA]^GATC[CT] 478 AGATCC MseI 134 TTAA T^TAA 604 TTAA NlaIII 297 CATG CATG^ 563 CATG NlaIV 239 GGGCCC GG[ACGT]^[ACGT]CC 361 GGTGCC PleI 46 GACTC GAGTC(4/5) 609 GAGTC SfaNI 331 GATGC GCATC(5/9) 161 GCATC Tru9I 134 TTAA T^TAA 604 TTAA XhoII 179 AGATCT [GA]^GATC[CT] 478 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AfaI BbvI BcnI BsaJI BssKI BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI HapII HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII RsaI Sau3AI Sau96I ScrFI TseI TspRI