(Linear) (Six-base) MAPSORT of: KIEE3990.seq  Check: 7442  from: 1  to: 624



KIEE3990.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     509     624 
   Size:        509     115
 
AccI GT'mk_AC

Cuts at:      0     155     624 
   Size:        155     469
 
AfeI AGC'GCT

Cuts at:      0     280     624 
   Size:        280     344
 
AflIII A'CryG_T

Cuts at:      0     618     624 
   Size:        618       6
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     184     624 
   Size:        184     440
 
AlwNI CAG_nnn'CTG

Cuts at:      0     545     624 
   Size:        545      79
 
ApaI G_GGCC'C

Cuts at:      0     243     624 
   Size:        243     381
 
BanI G'GyrC_C

Cuts at:      0     361     624 
   Size:        361     263
 
BanII G_rGCy'C

Cuts at:      0     243     624 
   Size:        243     381
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     148     451     624 
   Size:        148     303     173

  Fragments arranged by size: 

                 303     173     148
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     214     248     624 
   Size:        214      34     376

  Fragments arranged by size: 

                 376     214      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     167     624 
   Size:        167     457
 
BglII A'GATC_T

Cuts at:      0     179     624 
   Size:        179     445
 
Bme1580I G_kGCm'C

Cuts at:      0     243     624 
   Size:        243     381
 
BmgBI CAC'GTC

Cuts at:      0     315     624 
   Size:        315     309
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     117     381     624 
   Size:        117     264     243

  Fragments arranged by size: 

                 264     243     117
 
BsaBI GATnn'nnATC

Cuts at:      0     178     624 
   Size:        178     446
 
BsaHI Gr'CG_yC

Cuts at:      0     469     624 
   Size:        469     155
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      93     519     624 
   Size:         93     426     105

  Fragments arranged by size: 

                 426     105      93
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572     624 
   Size:        572      52
 
Bsp1286I G_dGCh'C

Cuts at:      0     243     624 
   Size:        243     381
 
BspHI T'CATG_A

Cuts at:      0     296     624 
   Size:        296     328
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      73     509     624 
   Size:         73     436     115

  Fragments arranged by size: 

                 436     115      73
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      65     624 
   Size:         65     559
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     437     624 
   Size:        437     187
 
BstYI r'GATC_y

Cuts at:      0     179     478     624 
   Size:        179     299     146

  Fragments arranged by size: 

                 299     179     146
 
BtsI GCAGTG_nn'

Cuts at:      0     188     340     624 
   Size:        188     152     284

  Fragments arranged by size: 

                 284     188     152
 
EarI CTCTTCn'nnn_

Cuts at:      0     521     624 
   Size:        521     103
 
HaeII r_GCGC'y

Cuts at:      0     282     624 
   Size:        282     342
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     322     354     624 
   Size:        322      32     270

  Fragments arranged by size: 

                 322     270      32
 
HincII GTy'rAC

Cuts at:      0     129     624 
   Size:        129     495
 
HindIII A'AGCT_T

Cuts at:      0     418     624 
   Size:        418     206
 
MluI A'CGCG_T

Cuts at:      0     618     624 
   Size:        618       6
 
MslI CAynn'nnrTG

Cuts at:      0     166     624 
   Size:        166     458
 
MspA1I CmG'CkG

Cuts at:      0     545     624 
   Size:        545      79
 
PasI CC'CwG_GG

Cuts at:      0     243     624 
   Size:        243     381
 
PfoI T'CCnGG_A

Cuts at:      0     412     624 
   Size:        412     212
 
PspOMI G'GGCC_C

Cuts at:      0     239     624 
   Size:        239     385
 
PstI C_TGCA'G

Cuts at:      0     195     624 
   Size:        195     429
 
PvuII CAG'CTG

Cuts at:      0     545     624 
   Size:        545      79
 
ScaI AGT'ACT

Cuts at:      0     229     624 
   Size:        229     395
 
SfcI C'TryA_G

Cuts at:      0     191     624 
   Size:        191     433
 
TatI w'GTAC_w

Cuts at:      0     227     624 
   Size:        227     397
 

 Enzymes that do cut:

     AarI     AccI     AfeI   AflIII     AlfI    AlwNI     ApaI     BanI
    BanII     BbsI     BcgI    BciVI    BglII Bme1580I    BmgBI     BplI
    BsaBI    BsaHI    BseRI     BsgI Bsp1286I    BspHI    BspMI   BstAPI
    BstXI    BstYI     BtsI     EarI    HaeII    Hin4I   HincII  HindIII
     MluI     MslI   MspA1I     PasI     PfoI   PspOMI     PstI    PvuII
     ScaI     SfcI     TatI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI     AleI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BclI    BfrBI     BglI     BlpI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MfeI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI