(Linear) (Six-base) MAPSORT of: KIEE3990.seq Check: 7442 from: 1 to: 624 KIEE3990.orf With 182 enzymes: SgfI * March 22, 2012 17:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 509 624 Size: 509 115 AccI GT'mk_AC Cuts at: 0 155 624 Size: 155 469 AfeI AGC'GCT Cuts at: 0 280 624 Size: 280 344 AflIII A'CryG_T Cuts at: 0 618 624 Size: 618 6 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 184 624 Size: 184 440 AlwNI CAG_nnn'CTG Cuts at: 0 545 624 Size: 545 79 ApaI G_GGCC'C Cuts at: 0 243 624 Size: 243 381 BanI G'GyrC_C Cuts at: 0 361 624 Size: 361 263 BanII G_rGCy'C Cuts at: 0 243 624 Size: 243 381 BbsI GAAGACnn'nnnn_ Cuts at: 0 148 451 624 Size: 148 303 173 Fragments arranged by size: 303 173 148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 214 248 624 Size: 214 34 376 Fragments arranged by size: 376 214 34 BciVI GTATCCnnnnn_n' Cuts at: 0 167 624 Size: 167 457 BglII A'GATC_T Cuts at: 0 179 624 Size: 179 445 Bme1580I G_kGCm'C Cuts at: 0 243 624 Size: 243 381 BmgBI CAC'GTC Cuts at: 0 315 624 Size: 315 309 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 117 381 624 Size: 117 264 243 Fragments arranged by size: 264 243 117 BsaBI GATnn'nnATC Cuts at: 0 178 624 Size: 178 446 BsaHI Gr'CG_yC Cuts at: 0 469 624 Size: 469 155 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 93 519 624 Size: 93 426 105 Fragments arranged by size: 426 105 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 572 624 Size: 572 52 Bsp1286I G_dGCh'C Cuts at: 0 243 624 Size: 243 381 BspHI T'CATG_A Cuts at: 0 296 624 Size: 296 328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 73 509 624 Size: 73 436 115 Fragments arranged by size: 436 115 73 BstAPI GCAn_nnn'nTGC Cuts at: 0 65 624 Size: 65 559 BstXI CCAn_nnnn'nTGG Cuts at: 0 437 624 Size: 437 187 BstYI r'GATC_y Cuts at: 0 179 478 624 Size: 179 299 146 Fragments arranged by size: 299 179 146 BtsI GCAGTG_nn' Cuts at: 0 188 340 624 Size: 188 152 284 Fragments arranged by size: 284 188 152 EarI CTCTTCn'nnn_ Cuts at: 0 521 624 Size: 521 103 HaeII r_GCGC'y Cuts at: 0 282 624 Size: 282 342 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 322 354 624 Size: 322 32 270 Fragments arranged by size: 322 270 32 HincII GTy'rAC Cuts at: 0 129 624 Size: 129 495 HindIII A'AGCT_T Cuts at: 0 418 624 Size: 418 206 MluI A'CGCG_T Cuts at: 0 618 624 Size: 618 6 MslI CAynn'nnrTG Cuts at: 0 166 624 Size: 166 458 MspA1I CmG'CkG Cuts at: 0 545 624 Size: 545 79 PasI CC'CwG_GG Cuts at: 0 243 624 Size: 243 381 PfoI T'CCnGG_A Cuts at: 0 412 624 Size: 412 212 PspOMI G'GGCC_C Cuts at: 0 239 624 Size: 239 385 PstI C_TGCA'G Cuts at: 0 195 624 Size: 195 429 PvuII CAG'CTG Cuts at: 0 545 624 Size: 545 79 ScaI AGT'ACT Cuts at: 0 229 624 Size: 229 395 SfcI C'TryA_G Cuts at: 0 191 624 Size: 191 433 TatI w'GTAC_w Cuts at: 0 227 624 Size: 227 397 Enzymes that do cut: AarI AccI AfeI AflIII AlfI AlwNI ApaI BanI BanII BbsI BcgI BciVI BglII Bme1580I BmgBI BplI BsaBI BsaHI BseRI BsgI Bsp1286I BspHI BspMI BstAPI BstXI BstYI BtsI EarI HaeII Hin4I HincII HindIII MluI MslI MspA1I PasI PfoI PspOMI PstI PvuII ScaI SfcI TatI Enzymes that do not cut: AatII Acc65I AclI AcuI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglI BlpI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI MfeI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI